Literature DB >> 7028745

Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.

J B Olmsted.   

Abstract

A method is described for the affinity purification of antibodies using protein samples that have been electrophoretically transferred to diazotized paper. Using differentiated neuroblastoma cells as the protein sample and a heterogeneous anti-microtubule protein serum, antibodies were isolated that specifically bound only to tubulin on blots and that stained microtubule networks in cells. The general utility of this method for various types of applications is discussed.

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Year:  1981        PMID： 7028745

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  260 in total

1.  A novel plant kinesin-related protein specifically associates with the phragmoplast organelles.

Authors:  Y R Lee; H M Giang; B Liu
Journal:  Plant Cell       Date:  2001-11       Impact factor: 11.277

2.  CHL1 is a nuclear protein with an essential ATP binding site that exhibits a size-dependent effect on chromosome segregation.

Authors:  S L Holloway
Journal:  Nucleic Acids Res       Date:  2000-08-15       Impact factor: 16.971

3.  Analysis of dynamin isoforms in mammalian brain: dynamin-1 expression is spatially and temporally regulated during postnatal development.

Authors:  K Faire; F Trent; J M Tepper; E M Bonder
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-01       Impact factor: 11.205

4.  Human neutrophil gelatinase is a component of specific granules.

Authors:  M S Hibbs; D F Bainton
Journal:  J Clin Invest       Date:  1989-11       Impact factor: 14.808

5.  Human vimentin autoantibodies preferentially interact with a peptide of 30kD mol. wt, located close to the amino-terminal of the molecule.

Authors:  A Alcover; C Hernández; J Avila
Journal:  Clin Exp Immunol       Date:  1985-07       Impact factor: 4.330

6.  Unique conformation of cancer autoantigen B23 in hepatoma: a mechanism for specificity in the autoimmune response.

Authors:  Danielle B Ulanet; Michael Torbenson; Chi V Dang; Livia Casciola-Rosen; Antony Rosen
Journal:  Proc Natl Acad Sci U S A       Date:  2003-09-30       Impact factor: 11.205

7.  Human anti-centromere sera recognise a 19.5 kD non-histone chromosomal protein from HeLa cells.

Authors:  H H Guldner; H J Lakomek; F A Bautz
Journal:  Clin Exp Immunol       Date:  1984-10       Impact factor: 4.330

8.  The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase.

Authors:  B T Roberts; K A Farr; M A Hoyt
Journal:  Mol Cell Biol       Date:  1994-12       Impact factor: 4.272

9.  Human autoantibodies against the 230-kD bullous pemphigoid antigen (BPAG1) bind only to the intracellular domain of the hemidesmosome, whereas those against the 180-kD bullous pemphigoid antigen (BPAG2) bind along the plasma membrane of the hemidesmosome in normal human and swine skin.

Authors:  A Ishiko; H Shimizu; A Kikuchi; T Ebihara; T Hashimoto; T Nishikawa
Journal:  J Clin Invest       Date:  1993-04       Impact factor: 14.808

10.  Microtubule-binding proteins from carrot : I. Initial characterization and microtubule bundling.

Authors:  R J Cyr; B A Palevitz
Journal:  Planta       Date:  1989-02       Impact factor: 4.116
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