Literature DB >> 4033758

Changing the binding specificity of a repressor by redesigning an alpha-helix.

R P Wharton, M Ptashne.   

Abstract

We replaced amino acids on the 'outside', or solvent-exposed, surface of the DNA recognition alpha-helix of 434 repressor with the corresponding amino acids from the recognition helix of P22 repressor. The binding specificity of the resulting hybrid protein, as measured in vivo and in vitro, was that of P22 repressor.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 4033758     DOI： 10.1038/316601a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  37 in total

1.  Identification and genetic mapping of a homeobox gene to the 4p16.1 region of human chromosome 4.

Authors:  H S Stadler; B J Padanilam; K Buetow; J C Murray; M Solursh
Journal:  Proc Natl Acad Sci U S A       Date:  1992-12-01       Impact factor: 11.205

Review 2.  Chromosomal insertion sites for phages and plasmids.

Authors:  A M Campbell
Journal:  J Bacteriol       Date:  1992-12       Impact factor: 3.490

3.  Inhibition of superinfection and the evolution of viral latency.

Authors:  Thomas W Berngruber; Franz J Weissing; Sylvain Gandon
Journal:  J Virol       Date:  2010-07-21       Impact factor: 5.103

4.  Cloning and characterization of the MboII restriction-modification system.

Authors:  H Bocklage; K Heeger; B Müller-Hill
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

5.  DNA binding specificity of the Arc and Mnt repressors is determined by a short region of N-terminal residues.

Authors:  K L Knight; R T Sauer
Journal:  Proc Natl Acad Sci U S A       Date:  1989-02       Impact factor: 11.205

6.  Purification and characterization of the repressor of the shiga toxin-encoding bacteriophage 933W: DNA binding, gene regulation, and autocleavage.

Authors:  Astrid P Koudelka; Lisa A Hufnagel; Gerald B Koudelka
Journal:  J Bacteriol       Date:  2004-11       Impact factor: 3.490

7.  Flavivirus enzyme-substrate interactions studied with chimeric proteinases: identification of an intragenic locus important for substrate recognition.

Authors:  F Preugschat; E M Lenches; J H Strauss
Journal:  J Virol       Date:  1991-09       Impact factor: 5.103

Review 8.  The chemistry of regulation of genes and other things.

Authors:  Mark Ptashne
Journal:  J Biol Chem       Date:  2014-01-02       Impact factor: 5.157

9.  The GCN4 bZIP targets noncognate gene regulatory sequences: quantitative investigation of binding at full and half sites.

Authors:  I-San Chan; Anna V Fedorova; Jumi A Shin
Journal:  Biochemistry       Date:  2007-02-13       Impact factor: 3.162

10.  DNA-mediated assembly of weakly interacting DNA-binding protein subunits: in vitro recruitment of phage 434 repressor and yeast GCN4 DNA-binding domains.

Authors:  Corrado Guarnaccia; Bakthisaran Raman; Sotir Zahariev; András Simoncsits; Sándor Pongor
Journal:  Nucleic Acids Res       Date:  2004-09-23       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.