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Literature DB >> 2644643

DNA binding specificity of the Arc and Mnt repressors is determined by a short region of N-terminal residues.

Abstract

The Arc and Mnt repressors of phage P22 are related proteins that bind to different operator DNA sites. By creating a hybrid Arc-Mnt protein, we show that the binding specificity of Mnt can be switched to that of Arc by replacing six residues at the N terminus of Mnt with the corresponding nine residues from Arc.

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Year: 1989 PMID： 2644643 PMCID： PMC286564 DOI： 10.1073/pnas.86.3.797

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

14 in total

1. Role of antirepressor in the bipartite control of repression and immunity by bacteriophage P22.

Authors: K Botstein; K K Lew; V Jarvik; C A Swanson
Journal: J Mol Biol Date: 1975-02-05 Impact factor: 5.469

2. Isolation and analysis of arc repressor mutants: evidence for an unusual mechanism of DNA binding.

Authors: A K Vershon; J U Bowie; T M Karplus; R T Sauer
Journal: Proteins Date: 1986-12

3. Bacteriophage P22 Mnt repressor. DNA binding and effects on transcription in vitro.

Authors: A K Vershon; S M Liao; W R McClure; R T Sauer
Journal: J Mol Biol Date: 1987-05-20 Impact factor: 5.469

4. Interaction of the bacteriophage P22 Arc repressor with operator DNA.

Authors: A K Vershon; S M Liao; W R McClure; R T Sauer
Journal: J Mol Biol Date: 1987-05-20 Impact factor: 5.469

5. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.

Authors: M Fried; D M Crothers
Journal: Nucleic Acids Res Date: 1981-12-11 Impact factor: 16.971

6. Changing the binding specificity of a repressor by redesigning an alpha-helix.

Authors: R P Wharton; M Ptashne
Journal: Nature Date: 1985 Aug 15-21 Impact factor: 49.962

7. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.

Authors: E Amann; J Brosius; M Ptashne
Journal: Gene Date: 1983-11 Impact factor: 3.688

8. The Mnt repressor of bacteriophage P22: role of C-terminal residues in operator binding and tetramer formation.

Authors: K L Knight; R T Sauer
Journal: Biochemistry Date: 1988-03-22 Impact factor: 3.162

9. Changing the DNA-binding specificity of a repressor.

Authors: P Youderian; A Vershon; S Bouvier; R T Sauer; M M Susskind
Journal: Cell Date: 1983-12 Impact factor: 41.582

10. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.

Authors: M M Garner; A Revzin
Journal: Nucleic Acids Res Date: 1981-07-10 Impact factor: 16.971

20 in total

1. Non-independence of Mnt repressor-operator interaction determined by a new quantitative multiple fluorescence relative affinity (QuMFRA) assay.

Authors: T K Man; G D Stormo
Journal: Nucleic Acids Res Date: 2001-06-15 Impact factor: 16.971

2. AmrZ beta-sheet residues are essential for DNA binding and transcriptional control of Pseudomonas aeruginosa virulence genes.

Authors: Elizabeth A Waligora; Deborah M Ramsey; Edward E Pryor; Haiping Lu; Thomas Hollis; Gina P Sloan; Rajendar Deora; Daniel J Wozniak
Journal: J Bacteriol Date: 2010-08-13 Impact factor: 3.490

DNA binding specificity of the Arc and Mnt repressors is determined by a short region of N-terminal residues.

1. Role of antirepressor in the bipartite control of repression and immunity by bacteriophage P22.

2. Isolation and analysis of arc repressor mutants: evidence for an unusual mechanism of DNA binding.

3. Bacteriophage P22 Mnt repressor. DNA binding and effects on transcription in vitro.

4. Interaction of the bacteriophage P22 Arc repressor with operator DNA.

5. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.

6. Changing the binding specificity of a repressor by redesigning an alpha-helix.

7. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.

8. The Mnt repressor of bacteriophage P22: role of C-terminal residues in operator binding and tetramer formation.

9. Changing the DNA-binding specificity of a repressor.

10. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.

1. Non-independence of Mnt repressor-operator interaction determined by a new quantitative multiple fluorescence relative affinity (QuMFRA) assay.

2. AmrZ beta-sheet residues are essential for DNA binding and transcriptional control of Pseudomonas aeruginosa virulence genes.

3. Specificity of the Mnt protein determined by binding to randomized operators.

4. A case study of the dynamics of in vitro DNA evolution under constant selection pressure.

5. Mutagenic dissection of the sequence determinants of protein folding, recognition, and machine function.

6. Searching sequence space by definably random mutagenesis: improving the catalytic potency of an enzyme.

7. Extensive DNA-binding specificity divergence of a conserved transcription regulator.

8. Helicobacter pylori NikR protein exhibits distinct conformations when bound to different promoters.

9. Identifying determinants of folding and activity for a protein of unknown structure.

10. Functional domains of the penicillinase repressor of Bacillus licheniformis.