Literature DB >> 401500

Two-dimensional restriction analysis of the Bacillus subtilis genome: gene purification and ribosomal ribonucleic acid gene organization.

S S Potter, K F Bott, J E Newbold.   

Abstract

With two-dimensional restriction enzyme analysis we have been able to cleave the Bacillus subtilis genome and resolve the resulting deoxyribonucleic acid (DNA) segments into discrete bands on agarose gels. A general procedure for gene purification has been developed by coupling multidimensional restriction analysis with a biological assay for gene detection. The organization of ribosomal ribonucleic acid (rRNA) genes was studied by hybridizing 16S and 23S rRNA probes to the two-dimensional DNA banding patterns.

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Year:  1977        PMID： 401500      PMCID： PMC234948          DOI： 10.1128/jb.129.1.492-500.1977

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  22 in total

1.  Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.

Authors:  M Thomas; R W Davis
Journal:  J Mol Biol       Date:  1975-01-25       Impact factor: 5.469

2.  A freeze-squeeze method for recovering long DNA from agarose gels.

Authors:  R W Thuring; J P Sanders; P Borst
Journal:  Anal Biochem       Date:  1975-05-26       Impact factor: 3.365

3.  Multidimensional restriction enzyme analysis of complex genomes.

Authors:  S S Potter; J E Newbold
Journal:  Anal Biochem       Date:  1976-04       Impact factor: 3.365

4.  Specificity in bacterial protein synthesis: role of initiation factors and ribosomal subunits.

Authors:  H F Lodish
Journal:  Nature       Date:  1970-05-23       Impact factor: 49.962

5.  Chromosomal location of DNA base sequences complementary to transfer RNA and to 5 s, 16 s and 23 s ribosomal RNA in Bacillus subtilis.

Authors:  I Smith; D Dubnau; P Morrell; J Marmur
Journal:  J Mol Biol       Date:  1968-04-14       Impact factor: 5.469

6.  Visualization of prokaryotic DNA in a regularly condensed chromatin-like fiber.

Authors:  J D Griffith
Journal:  Proc Natl Acad Sci U S A       Date:  1976-02       Impact factor: 11.205

7.  Electrophoretic separation of Bacillus subtilis genes.

Authors:  R M Harris-Warrick; Y Elkana; S D Ehrlich; J Lederberg
Journal:  Proc Natl Acad Sci U S A       Date:  1975-06       Impact factor: 11.205

8.  Determinant of cistron specificity in bacterial ribosomes.

Authors:  J Shine; L Dalgarno
Journal:  Nature       Date:  1975-03-06       Impact factor: 49.962

9.  Change in the template specificity of RNA polymerase during sporulation of Bacillus subtilis.

Authors:  R Losick; A L Sonenshein
Journal:  Nature       Date:  1969-10-04       Impact factor: 49.962

10.  Plasmid deoxyribonucleic acid in Bacillus subtilis and Bacillus pumilus.

Authors:  P S Lovett; M G Bramucci
Journal:  J Bacteriol       Date:  1975-10       Impact factor: 3.490
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  11 in total

1.  Hybridization analysis of restriction endonuclease DNA fragments of Bacillus cereus transcribed during spore outgrowth.

Authors:  Z Silberstein; A Cohen
Journal:  J Bacteriol       Date:  1978-06       Impact factor: 3.490

2.  Tn5-induced mutants of Azotobacter vinelandii affected in nitrogen fixation under Mo-deficient and Mo-sufficient conditions.

Authors:  R D Joerger; R Premakumar; P E Bishop
Journal:  J Bacteriol       Date:  1986-11       Impact factor: 3.490

3.  Heterogeneity of the genes coding for 5 S RNA in three related strains of the genus Bacillus.

Authors:  H A Raué; A Rosner; R J Planta
Journal:  Mol Gen Genet       Date:  1977-11-14

4.  A restriction map of the bacteriophage T4 genome.

Authors:  P H O'Farrell; E Kutter; M Nakanishi
Journal:  Mol Gen Genet       Date:  1980

5.  tRNA genes are found between 16S and 23S rRNA genes in Bacillus subtilis.

Authors:  K Loughney; E Lund; J E Dahlberg
Journal:  Nucleic Acids Res       Date:  1982-03-11       Impact factor: 16.971

6.  Construction of a colony bank of E. coli containing hybrid plasmids representative of the Bacillus subtilis 168 genome. Expression of functions harbored by the recombinant plasmids in B. subtilis.

Authors:  G Rapoport; A Klier; A Billault; F Fargette; R Dedonder
Journal:  Mol Gen Genet       Date:  1979-10-03

7.  Restriction enzyme analysis of Bacillus subtilis ribosomal ribonucleic acid genes.

Authors:  C P Moran; K F Bott
Journal:  J Bacteriol       Date:  1979-10       Impact factor: 3.490

8.  Different levels of DNA modification at 5'CCGG in murine erythroleukemia cells and the tissues of normal mouse spleen.

Authors:  S S Smith; J C Yu; C W Chen
Journal:  Nucleic Acids Res       Date:  1982-07-24       Impact factor: 16.971

9.  Structure of cloned ribosomal DNA cistrons from Bacillus thuringiensis.

Authors:  A F Klier; F Kunst; G Rapoport
Journal:  Nucleic Acids Res       Date:  1979-10-25       Impact factor: 16.971

10.  Molecular cloning of nif DNA from Azotobacter vinelandii.

Authors:  P E Bishop; T M Rizzo; K F Bott
Journal:  J Bacteriol       Date:  1985-04       Impact factor: 3.490
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