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Literature DB >> 388353

Structure of cloned ribosomal DNA cistrons from Bacillus thuringiensis.

Abstract

A library of B. thuringiensis DNA has been prepared by using the plasmid pBR322 as a cloning vehicle and E. coli as a host cell. By screening this collection with specific probes, 17 clones were identified whose hybrid plasmids contain rRNA genes of B. thuringiensis. Several of these plasmids have been mapped with restriction endonucleases and by DNA-RNA hybridization. By using maps of overlapping fragments, we have been able to establish an overall map of the ribosomal gene cluster.

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Year: 1979 PMID： 388353 PMCID： PMC342277 DOI： 10.1093/nar/7.4.997

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

22 in total

1. EQUILIBRIUM SEDIMENTATION OF MACROMOLECULES IN DENSITY GRADIENTS.

Authors: M Meselson; F W Stahl; J Vinograd
Journal: Proc Natl Acad Sci U S A Date: 1957-07-15 Impact factor: 11.205

2. Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli.

Authors: M L Palmer; M A Raker; P J Kennedy; J W Young; W M Barnes; R L Rodriguez; H F Noller
Journal: Mol Gen Genet Date: 1979-05-04

3. Rapid purification of plasmid DNAs by hydroxyapatite chromatography.

Authors: A Colman; M J Byers; S B Primrose; A Lyons
Journal: Eur J Biochem Date: 1978-11-02

4. Physical map of the seven ribosomal RNA genes of Escherichia coli.

Authors: I Boros; A Kiss; P Venetianer
Journal: Nucleic Acids Res Date: 1979 Impact factor: 16.971

5. Characterization of hybrid plasmids carrying individual ribosomal ribonucleic acid transcription units of Escherichia coli.

Authors: M E Kenerley; E A Morgan; L Post; L Lindahl; M Nomura
Journal: J Bacteriol Date: 1977-12 Impact factor: 3.490

6. Two-dimensional restriction analysis of the Bacillus subtilis genome: gene purification and ribosomal ribonucleic acid gene organization.

Authors: S S Potter; K F Bott; J E Newbold
Journal: J Bacteriol Date: 1977-01 Impact factor: 3.490

7. A method for the recovery of DNA from agarose gels.

Authors: H F Tabak; R A Flavell
Journal: Nucleic Acids Res Date: 1978-07 Impact factor: 16.971

8. A ribosomal RNA gene, rrnC, of Escherichia coli, mapped by specialized transducing lambdadilv and lambda drbs phages.

Authors: P Jørgensen; J Collins; N Fiil; K von Meyenbourg
Journal: Mol Gen Genet Date: 1978-07-11

9. Complementary sequences 1700 nucleotides apart form a ribonuclease III cleavage site in Escherichia coli ribosomal precursor RNA.

Authors: R A Young; J A Steitz
Journal: Proc Natl Acad Sci U S A Date: 1978-08 Impact factor: 11.205

10. Processing of rRNA by RNAase P: spacer tRNAs are linked to 16S rRNA in an RNAase P RNAase III mutant strain of E. coli.

Authors: P Gegenheimer; D Apirion
Journal: Cell Date: 1978-10 Impact factor: 41.582

6 in total

Structure of cloned ribosomal DNA cistrons from Bacillus thuringiensis.

1. EQUILIBRIUM SEDIMENTATION OF MACROMOLECULES IN DENSITY GRADIENTS.

2. Isolation and restriction mapping of plasmids containing ribosomal DNA sequences from the rrn B cistron of E. coli.

3. Rapid purification of plasmid DNAs by hydroxyapatite chromatography.

4. Physical map of the seven ribosomal RNA genes of Escherichia coli.

5. Characterization of hybrid plasmids carrying individual ribosomal ribonucleic acid transcription units of Escherichia coli.

6. Two-dimensional restriction analysis of the Bacillus subtilis genome: gene purification and ribosomal ribonucleic acid gene organization.

7. A method for the recovery of DNA from agarose gels.

8. A ribosomal RNA gene, rrnC, of Escherichia coli, mapped by specialized transducing lambdadilv and lambda drbs phages.

9. Complementary sequences 1700 nucleotides apart form a ribonuclease III cleavage site in Escherichia coli ribosomal precursor RNA.

10. Processing of rRNA by RNAase P: spacer tRNAs are linked to 16S rRNA in an RNAase P RNAase III mutant strain of E. coli.

1. Characterization of Vibrio fischeri rRNA operons and subcloning of a ribosomal DNA promoter.

2. Organisation of the ribosomal RNA genes in Streptomyces coelicolor A3(2).

Review 3. Has the endosymbiont hypothesis been proven?

4. Cloning and expression in Escherichia coli of the sucrase gene from Bacillus subtilis.

5. Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control.

6. Cloning and expression of the crystal protein genes from Bacillus thuringiensis strain berliner 1715.