Literature DB >> 3995563

Isolation and partial characterization of two populations of secretory granules from rat parotid glands.

J M Iversen, D L Kauffman, P J Keller, M Robinovitch.   

Abstract

A method is described for the isolation of two populations of secretory granules from rat parotid glands utilizing differences in their sedimentation characteristics. The granule preparations were analyzed for homogeneity by electron microscopy and chemical analyses. The soluble contents of both types of granules were obtained by hypotonic lysis, and the proteins compared by SDS-PAGE and ion exchange-gel filtration chromatography. Both populations of secretory granules appear to have the same protein composition as that of the parotid saliva. The secretory granules with the smaller apparent buoyant density became labelled with radioactive leucine earlier than the heavier granules when a pulse of this amino acid was supplied to a gland slice system. The lighter granules appear to represent an earlier stage in maturation.

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Year:  1985        PMID： 3995563     DOI： 10.1007/bf00222357

Source DB:  PubMed          Journal:  Cell Tissue Res        ISSN： 0302-766X            Impact factor:   5.249


  29 in total

1.  A microspectrophotometric method for the determination of cytochrome oxidase.

Authors:  S J COOPERSTEIN; A LAZAROW
Journal:  J Biol Chem       Date:  1951-04       Impact factor: 5.157

2.  Quantitative study of secretory granules of the parotid gland acinar cell of the rat.

Authors:  T Itoh
Journal:  J Electron Microsc (Tokyo)       Date:  1977

3.  Purification of secretory granules on a urografin gradient.

Authors:  N Kirshner; D Wallach; Y Sharoni; M Schramm
Journal:  Anal Biochem       Date:  1973-04       Impact factor: 3.365

4.  Preservation of the secretory granules of rat parotid gland for electron microscopy.

Authors:  M R Robinovitch; L M Sreebny; E A Smuckler
Journal:  Exp Cell Res       Date:  1966-06       Impact factor: 3.905

5.  The protein composition of rat parotid saliva and secretory granules.

Authors:  P J Keller; M Robinovitch; J Iversen; D L Kauffman
Journal:  Biochim Biophys Acta       Date:  1975-02-27

6.  Isolation and characterization of the basic proline-rich proteins from rat parotid saliva.

Authors:  J M Iversen; D A Johnson; D L Kauffman; P J Keller; M R Robinovitch
Journal:  Arch Oral Biol       Date:  1982       Impact factor: 2.633

7.  Presence of sulfated proteoglycans in prolactin secretory granules isolated from the rat pituitary gland.

Authors:  G Giannattasio; A Zanini
Journal:  Biochim Biophys Acta       Date:  1976-08-09

8.  Secretion of old versus new exportable protein in rat parotid slics. Control by neurotransmitters.

Authors:  Y Sharoni; S Eimerl; M Schramm
Journal:  J Cell Biol       Date:  1976-10       Impact factor: 10.539

9.  Ionic interactions between bovine chymotrypsinogen A and chondroitin sulfate A.B.C.. A possible model for molecular aggregation in zymogen granules.

Authors:  H Reggio; J C Dagorn
Journal:  J Cell Biol       Date:  1978-09       Impact factor: 10.539

10.  Molecular organization of prolactin granules. III. Intracellular transport of sulfated glycosaminoglycans and glycoproteins of the bovine prolactin granule matrix.

Authors:  G Giannattasio; A Zanini; P Rosa; J Meldolesi; R K Margolis; R U margolis
Journal:  J Cell Biol       Date:  1980-07       Impact factor: 10.539
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  8 in total

1.  Evidence for G proteins in rat parotid plasma membranes and secretory granule membranes.

Authors:  E L Watson; D DiJulio; D Kauffman; J Iversen; M R Robinovitch; K T Izutsu
Journal:  Biochem J       Date:  1992-07-15       Impact factor: 3.857

2.  Maturation-related changes in mass and elemental contents of secretory granules as measured by electron-microprobe.

Authors:  K T Izutsu; M K Goddard; J M Iversen; M R Robinovitch; T K Oswald; M Cantino; D Johnson
Journal:  Cell Tissue Res       Date:  1991-03       Impact factor: 5.249

3.  Lectin-binding pattern in parotid acinar cells. The fracture-labelling method and post-embedding staining.

Authors:  K Jezernik; N Pipan
Journal:  Histochemistry       Date:  1986

4.  The presence of chondroitin sulfate in parotid secretory granules and saliva of the rat.

Authors:  J M Iversen; P J Keller; D L Kauffman; M R Robinovitch
Journal:  Cell Tissue Res       Date:  1987-10       Impact factor: 5.249

5.  Immunocytochemical localization of seminal proteins in salivary and lacrimal glands of the rat.

Authors:  G Aumüller; E A Arce; W Heyns; I Vercaeren; I Dammshäuser; J Seitz
Journal:  Cell Tissue Res       Date:  1995-04       Impact factor: 5.249

6.  Isolated secretion granules from parotid glands of chronically stimulated rats possess an alkaline internal pH and inward-directed H+ pump activity.

Authors:  P Arvan; J D Castle
Journal:  J Cell Biol       Date:  1986-10       Impact factor: 10.539

7.  Protein sorting among two distinct export pathways occurs from the content of maturing exocrine storage granules.

Authors:  M von Zastrow; J D Castle
Journal:  J Cell Biol       Date:  1987-12       Impact factor: 10.539

8.  β-adrenoceptor activation increased VAMP-2 and syntaxin-4 in secretory granules are involved in protein secretion of submandibular gland through the PKA/F-actin pathway.

Authors:  Chong Ding; Xin Cong; Yan Zhang; Sheng-Lin Li; Li-Ling Wu; Guang-Yan Yu
Journal:  Biosci Rep       Date:  2018-02-13       Impact factor: 3.840
  8 in total

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