Literature DB >> 3987679

A micromethod for endotoxin assay in human plasma using Limulus amoebocyte lysate and a chromogenic substrate.

B I Piotrowicz, I Watt, S Edlin, A C McCartney.   

Abstract

A modified micromethod for endotoxin assay is reported which involved incubation of plasma extract with Limulus amoebocyte lysate for 30 min followed by incubation with chromogenic substrate S-2423 for another 8 min. Absorbance is measured in microtitre plates. The sensitivity is less than 5 pg/ml (0.058 Endotoxin Units/ml).

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Year:  1985        PMID： 3987679     DOI： 10.1007/bf02148661

Source DB:  PubMed          Journal:  Eur J Clin Microbiol        ISSN： 0722-2211            Impact factor:   3.267


  9 in total

1.  A sensitive substrate for the clotting enzyme in horseshoe crab hemocytes.

Authors:  S Nakamura; T Morita; S Iwanaga; M Niwa; K Takahashi
Journal:  J Biochem       Date:  1977-05       Impact factor: 3.387

2.  THE ROLE OF ENDOTOXIN IN THE EXTRACELLULAR COAGULATION OF LIMULUS BLOOD.

Authors:  J LEVIN; F B BANG
Journal:  Bull Johns Hopkins Hosp       Date:  1964-09

3.  Distribution and clearance of circulating endotoxin.

Authors:  W B HERRING; J C HERION; R I WALKER; J G PALMER
Journal:  J Clin Invest       Date:  1963-01       Impact factor: 14.808

4.  Clearance of endotoxin by platelets: role in increasing the accuracy of the Limulus gelation test and in combating experimental endotoxemia.

Authors:  J Das; A A Schwartz; J Folkman
Journal:  Surgery       Date:  1973-08       Impact factor: 3.982

5.  An improved chromogenic substrate endotoxin assay for clinical use.

Authors:  R I Harris; P C Stone; J Stuart
Journal:  J Clin Pathol       Date:  1983-10       Impact factor: 3.411

6.  Quantitative determination of endotoxin in plasma by means of a new chromogenic peptide substrate. A methodological study.

Authors:  S Aune; A O Aasen; P Kierulf; J H Strømme
Journal:  Acta Chir Scand Suppl       Date:  1982

7.  Quantitative measurement of endotoxin in human blood using synthetic chromogenic substrate for horseshoe crab clotting enzyme: a comparison of methods of blood sampling and treatment.

Authors:  K Takagi; A Moriya; H Tamura; C Nakahara; S Tanaka; V Fujita; T Kawai
Journal:  Thromb Res       Date:  1981 Jul 1-15       Impact factor: 3.944

8.  Quantitative endotoxin determination in blood with a chromogenic substrate.

Authors:  L L Thomas; A Sturk; L H Kahlé; J W ten Cate
Journal:  Clin Chim Acta       Date:  1981-10-08       Impact factor: 3.786

9.  Endotoxaemia in septic shock: clinical and post mortem correlations.

Authors:  A C McCartney; J G Banks; G B Clements; J D Sleigh; M Tehrani; I M Ledingham
Journal:  Intensive Care Med       Date:  1983       Impact factor: 17.440
  9 in total
  1 in total

1.  Endotoxin testing revisited.

Authors:  A Sturk; J W ten Cate
Journal:  Eur J Clin Microbiol       Date:  1985-08       Impact factor: 3.267
  1 in total

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