Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Quantitative endotoxin determination in blood with a chromogenic substrate.

Literature DB >> 7318174

Quantitative endotoxin determination in blood with a chromogenic substrate.

L L Thomas, A Sturk, L H Kahlé, J W ten Cate.

Abstract

A method is described for the quantitative determination of endotoxins in blood. The method is based upon the endotoxin-dependent activation of a proenzyme present in Limulus amebocyte lysate. This activated enzyme is measured by using the chromogenic substrate S 2422. Inhibitors and activated coagulation factors possibly interfering in the assay are removed by dilution and boiling. The method has been proven to be fast (2.5 h), sensitive and reproducible with a detection limit of 10 ng/l. Preliminary results comparing the results of blood cultures with the endotoxin assay indicate a good correlation.

Mesh：

Substances：

Year: 1981 PMID： 7318174 DOI： 10.1016/0009-8981(81)90169-8

Source DB: PubMed Journal: Clin Chim Acta ISSN： 0009-8981 Impact factor: 3.786

Keyword Cloud
Cited

13 in total

Review 1. [Endotoxins. Pathogenetic meaning of sepsis].

Authors: H Rensing
Journal: Anaesthesist Date: 2003-12 Impact factor: 1.041

2. Use of two-dimensional gas chromatography with electron-capture detection for the measurement of lipopolysaccharides in peritoneal fluid and plasma from rats with induced peritonitis.

Authors: A Sonesson; L Larsson; R Andersson; N Adner; K G Tranberg
Journal: J Clin Microbiol Date: 1990-06 Impact factor: 5.948

10. Role of endotoxemia in cardiovascular dysfunction and lethality: virulent and nonvirulent Escherichia coli challenges in a canine model of septic shock.

Authors: W D Hoffman; R L Danner; Z M Quezado; S M Banks; R J Elin; J M Hosseini; C Natanson
Journal: Infect Immun Date: 1996-02 Impact factor: 3.441

Quantitative endotoxin determination in blood with a chromogenic substrate.

Review 1. [Endotoxins. Pathogenetic meaning of sepsis].

2. Use of two-dimensional gas chromatography with electron-capture detection for the measurement of lipopolysaccharides in peritoneal fluid and plasma from rats with induced peritonitis.

3. A micromethod for endotoxin assay in human plasma using Limulus amoebocyte lysate and a chromogenic substrate.

4. Measurement of endotoxaemia by the Limulus test.

5. Endotoxin testing revisited.

6. The Limulus Amoebocyte Lysate test: detection of endotoxin in plasma of swine and cattle. I. In vitro investigations.

7. The Limulus Amoebocyte Lysate test: detection of endotoxin in plasma of swine and cattle. II. In vivo investigations.

8. Chromogenic endotoxin determination in blood--clinical relevance.

9. Turbidimetric method for quantifying serum inhibition of Limulus amoebocyte lysate.

10. Role of endotoxemia in cardiovascular dysfunction and lethality: virulent and nonvirulent Escherichia coli challenges in a canine model of septic shock.