Literature DB >> 3977856

Resolution and reconstitution of bovine kidney branched-chain 2-oxo acid dehydrogenase complex.

K G Cook, A P Bradford, S J Yeaman.   

Abstract

Branched-chain 2-oxo acid dehydrogenase complex was resolved into component E1 and E2-kinase subcomplex by gel filtration in the presence of 1 M-NaC1. Essentially all the original activity of the complex can be regained after reconstitution of the component enzymes, reassociation being a rapid process. The specific activities of E1 and E2 were 25.1 and 19.0 units/mg respectively. Non-phosphorylated active E1 has an approx. 6-fold higher affinity for E2 than does phosphorylated E1. The components of the branched-chain 2-oxo acid dehydrogenase complex do not crossreact with the respective components from the pyruvate dehydrogenase complex. The significance of these results and of the tight association of the kinase with E2 are discussed.

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Year:  1985        PMID： 3977856      PMCID： PMC1144650          DOI： 10.1042/bj2250731

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  14 in total

1.  -Keto acid dehydrogenase complexes. XVI. Studies on the subunit structure of the pyruvate dehydrogenase complexes from bovine kidney and heart.

Authors:  C R Barrera; G Namihira; L Hamilton; P Munk; M H Eley; T C Linn; L J Reed
Journal:  Arch Biochem Biophys       Date:  1972-02       Impact factor: 4.013

2.  -Keto acid dehydrogenase complexes. XV. Purification and properties of the component enzymes of the pyruvate dehydrogenase complexes from bovine kidney and heart.

Authors:  T C Linn; J W Pelley; F H Pettit; F Hucho; D D Randall; L J Reed
Journal:  Arch Biochem Biophys       Date:  1972-02       Impact factor: 4.013

3.  Enhanced dissociation of pyruvate dehydrogenase from the pyruvate dehydrogenase complex following phosphorylation and regulatory implications.

Authors:  M L Pratt; T E Roche; D W Dyer; R L Cate
Journal:  Biochem Biophys Res Commun       Date:  1979-11-14       Impact factor: 3.575

4.  Purification and characterization of branched chain alpha-keto acid dehydrogenase complex of bovine kidney.

Authors:  F H Pettit; S J Yeaman; L J Reed
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

5.  Purification of rat kidney branched-chain oxo acid dehydrogenase complex with endogenous kinase activity.

Authors:  R Odessey
Journal:  Biochem J       Date:  1982-04-15       Impact factor: 3.857

6.  Subunit binding in the pyruvate dehydrogenase complex from bovine kidney and heart.

Authors:  T L Wu; L J Reed
Journal:  Biochemistry       Date:  1984-01-17       Impact factor: 3.162

7.  Rapid intersite transfer of acetyl groups and movement of pyruvate dehydrogenase component in the kidney pyruvate dehydrogenase complex.

Authors:  R L Cate; T E Roche; L C Davis
Journal:  J Biol Chem       Date:  1980-08-25       Impact factor: 5.157

8.  Rapid purification of bovine kidney branched-chain 2-oxoacid dehydrogenase complex containing endogenous kinase activity.

Authors:  R Lawson; K G Cook; S J Yeaman
Journal:  FEBS Lett       Date:  1983-06-27       Impact factor: 4.124

9.  Maturation of the head of bacteriophage T4. I. DNA packaging events.

Authors:  U K Laemmli; M Favre
Journal:  J Mol Biol       Date:  1973-11-15       Impact factor: 5.469

10.  Inactivation of purified ox kidney branched-chain 2-oxoacid dehydrogenase complex by phosphorylation.

Authors:  H R Fatania; K S Lau; P J Randle
Journal:  FEBS Lett       Date:  1981-09-28       Impact factor: 4.124
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  13 in total

1.  Production of recombinant E1 component of branched-chain alpha-keto acid dehydrogenase complex.

Authors:  J W Hawes; Y Zhao; K M Popov; Y Shimomura; R A Harris
Journal:  Methods Enzymol       Date:  2000       Impact factor: 1.600

Review 2.  The 2-oxo acid dehydrogenase complexes: recent advances.

Authors:  S J Yeaman
Journal:  Biochem J       Date:  1989-02-01       Impact factor: 3.857

3.  Impaired growth and neurological abnormalities in branched-chain alpha-keto acid dehydrogenase kinase-deficient mice.

Authors:  Mandar A Joshi; Nam Ho Jeoung; Mariko Obayashi; Eyas M Hattab; Eric G Brocken; Edward A Liechty; Michael J Kubek; Krishna M Vattem; Ronald C Wek; Robert A Harris
Journal:  Biochem J       Date:  2006-11-15       Impact factor: 3.857

4.  Resolution of branched-chain oxo acid dehydrogenase complex of Pseudomonas aeruginosa PAO.

Authors:  V McCully; G Burns; J R Sokatch
Journal:  Biochem J       Date:  1986-02-01       Impact factor: 3.857

5.  Cloning and cDNA sequence of the dihydrolipoamide dehydrogenase component human alpha-ketoacid dehydrogenase complexes.

Authors:  G Pons; C Raefsky-Estrin; D J Carothers; R A Pepin; A A Javed; B W Jesse; M K Ganapathi; D Samols; M S Patel
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205

6.  T cell responses to the putative dominant autoepitope in primary biliary cirrhosis (PBC).

Authors:  J M Palmer; A G Diamond; S J Yeaman; M F Bassendine; D E Jones
Journal:  Clin Exp Immunol       Date:  1999-04       Impact factor: 4.330

7.  Maple syrup urine disease (MSUD): screening for known mutations in Italian patients.

Authors:  T Parrella; S Surrey; A Iolascon; M Sartore; R Heidenreich; G Diamond; A Ponzone; O Guardamagna; A B Burlina; R Cerone
Journal:  J Inherit Metab Dis       Date:  1994       Impact factor: 4.982

8.  Maple syrup urine disease. Complete primary structure of the E1 beta subunit of human branched chain alpha-ketoacid dehydrogenase complex deduced from the nucleotide sequence and a gene analysis of patients with this disease.

Authors:  Y Nobukuni; H Mitsubuchi; F Endo; I Akaboshi; J Asaka; I Matsuda
Journal:  J Clin Invest       Date:  1990-07       Impact factor: 14.808

9.  Rat tissue concentrations of branched-chain 2-oxo acid dehydrogenase complex. Re-evaluation by immunoassay and bioassay.

Authors:  P A Patston; J Espinal; J M Shaw; P J Randle
Journal:  Biochem J       Date:  1986-04-15       Impact factor: 3.857

10.  Identification and analysis of the major M2 autoantigens in primary biliary cirrhosis.

Authors:  S P Fussey; J R Guest; O F James; M F Bassendine; S J Yeaman
Journal:  Proc Natl Acad Sci U S A       Date:  1988-11       Impact factor: 11.205
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