Literature DB >> 6288017

Purification of rat kidney branched-chain oxo acid dehydrogenase complex with endogenous kinase activity.

R Odessey.   

Abstract

A method was devised to purify branched-chain oxo acid dehydrogenase (BCOAD) from rat kidney which retains endogenous kinase activity. Incorporation of 32P into purified enzyme parallels the time course of enzyme inhibition by ATP. Phosphorylation occurs on a serine residue(s) of the 46000-mol.wt. subunit of the enzyme complex. Endogenous phosphatase activity is not present after purification, and added pyruvate dehydrogenase phosphate phosphatase does not re-activate BCOAD or liberate 32P from previously labelled enzyme. These results demonstrate that BCOAD can be regulated by an endogenous protein kinase and that the phosphorylation-cycle enzymes regulating BCOAD appear to be distinct from those associated with pyruvate dehydrogenase complex.

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Year:  1982        PMID： 6288017      PMCID： PMC1158352          DOI： 10.1042/bj2040353

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  13 in total

1.  Multiple phosphorylation of rabbit skeletal muscle glycogen synthase. Comparison of the actions of different protein kinases capable of catalyzing phosphorylation in vitro.

Authors:  A A DePaoli-Roach; P J Roach; J Larner
Journal:  J Biol Chem       Date:  1979-12-10       Impact factor: 5.157

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Authors:  K P Huang; J C Robinson
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Activation of glycogen synthase in rat adipocytes by insulin and glucose involves increased glucose transport and phosphorylation.

Authors:  J C Lawrence; J Larner
Journal:  J Biol Chem       Date:  1978-04-10       Impact factor: 5.157

4.  Site of action of insulin in promoting leucine utilization in adipose tissue.

Authors:  H M Goodman
Journal:  Am J Physiol       Date:  1977-08

5.  Purification and characterization of branched chain alpha-keto acid dehydrogenase complex of bovine kidney.

Authors:  F H Pettit; S J Yeaman; L J Reed
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

6.  Active and inactive forms of branched-chain 2-oxoacid dehydrogenase complex in rat heart and skeletal muscle.

Authors:  P J Parker; P J Randle
Journal:  FEBS Lett       Date:  1980-04-07       Impact factor: 4.124

7.  The regulation of branched-chain 2-oxo acid dehydrogenase of liver, kidney and heart by phosphorylation.

Authors:  W A Hughes; A P Halestrap
Journal:  Biochem J       Date:  1981-05-15       Impact factor: 3.857

8.  Inactivation of rat heart branched-chain 2-oxoacid dehydrogenase complex by adenosine triphosphate.

Authors:  P J Parker; P J Randle
Journal:  FEBS Lett       Date:  1978-11-01       Impact factor: 4.124

9.  Oxidation of leucine by rat liver and kidney.

Authors:  A G Dawson; F J Hird; D J Morton
Journal:  Arch Biochem Biophys       Date:  1967-11       Impact factor: 4.013

10.  Purification and characterization of branched chain alpha-ketoacid dehydrogenase from bovine liver mitochondria.

Authors:  D J Danner; S K Lemmon; J C Besharse; L J Elsas
Journal:  J Biol Chem       Date:  1979-06-25       Impact factor: 5.157
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  25 in total

1.  Mechanism for basal expression of rat mitochondrial branched-chain-2-oxo-acid dehydrogenase kinase [corrected].

Authors:  Y S Huang; D T Chuang
Journal:  Biochem J       Date:  1998-09-15       Impact factor: 3.857

2.  Regulation of the branched-chain 2-oxo acid dehydrogenase complex in hepatocytes isolated from rats fed on a low-protein diet.

Authors:  R A Harris; R Paxton; G W Goodwin; S M Powell
Journal:  Biochem J       Date:  1986-03-01       Impact factor: 3.857

3.  Resolution of branched-chain oxo acid dehydrogenase complex of Pseudomonas aeruginosa PAO.

Authors:  V McCully; G Burns; J R Sokatch
Journal:  Biochem J       Date:  1986-02-01       Impact factor: 3.857

4.  A potent, heat-stable protein inhibitor of [branched-chain alpha-keto acid dehydrogenase]-phosphatase from bovine kidney mitochondria.

Authors:  Z Damuni; J S Humphreys; L J Reed
Journal:  Proc Natl Acad Sci U S A       Date:  1986-01       Impact factor: 11.205

5.  Preservation of the activity state of hepatic branched-chain 2-oxo acid dehydrogenase during the isolation of mitochondria.

Authors:  B Zhang; R Paxton; G W Goodwin; Y Shimomura; R A Harris
Journal:  Biochem J       Date:  1987-09-15       Impact factor: 3.857

6.  Phosphorylation of branched-chain 2-oxo acid dehydrogenase complex in isolated adipocytes. Effects of 2-oxo acids.

Authors:  S M Jones; S J Yeaman
Journal:  Biochem J       Date:  1986-05-15       Impact factor: 3.857

7.  The bkdR gene of Pseudomonas putida is required for expression of the bkd operon and encodes a protein related to Lrp of Escherichia coli.

Authors:  K T Madhusudhan; D Lorenz; J R Sokatch
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

8.  Maple syrup urine disease. Complete primary structure of the E1 beta subunit of human branched chain alpha-ketoacid dehydrogenase complex deduced from the nucleotide sequence and a gene analysis of patients with this disease.

Authors:  Y Nobukuni; H Mitsubuchi; F Endo; I Akaboshi; J Asaka; I Matsuda
Journal:  J Clin Invest       Date:  1990-07       Impact factor: 14.808

9.  Rat tissue concentrations of branched-chain 2-oxo acid dehydrogenase complex. Re-evaluation by immunoassay and bioassay.

Authors:  P A Patston; J Espinal; J M Shaw; P J Randle
Journal:  Biochem J       Date:  1986-04-15       Impact factor: 3.857

10.  A study of the metabolism of [U-14C]3-methyl-2-oxopentanoate by rat liver mitochondria using h.p.l.c. with continuous on-line monitoring of radioactive intact acyl-coenzyme A intermediates.

Authors:  A G Causey; B Middleton; K Bartlett
Journal:  Biochem J       Date:  1986-04-15       Impact factor: 3.857
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