Literature DB >> 3968212

Gas chromatographic identification of Clostridium difficile and detection of cytotoxin from a modified selective medium.

P N Levett, K D Phillips.   

Abstract

A modification of an existing selective medium for Clostridium difficile is described. Inclusion in the medium of DL nor-leucine and p-hydroxyphenylacetic acid enables identification of C difficile to be made directly from primary isolation plates by gas chromatographic detection of caproic acid and p-cresol. Plugs of agar withdrawn from the selective medium also allow the detection of cytotoxin production in vitro.

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Year:  1985        PMID: 3968212      PMCID: PMC499077          DOI: 10.1136/jcp.38.1.82

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN: 0021-9746            Impact factor:   3.411


  13 in total

1.  The amino acid-fermenting clostridia.

Authors:  G C Mead
Journal:  J Gen Microbiol       Date:  1971-07

2.  Simplified procedure for the routine isolation of Clostridium difficile from faeces.

Authors:  S P Borriello; P Honour
Journal:  J Clin Pathol       Date:  1981-10       Impact factor: 3.411

3.  The production of urinary phenols by gut bacteria and their possible role in the causation of large bowel cancer.

Authors:  E Bone; A Tamm; M Hill
Journal:  Am J Clin Nutr       Date:  1976-12       Impact factor: 7.045

4.  Immunological analysis of the EDTA-soluble antigens of Clostridium difficile and related species.

Authors:  I R Poxton; M D Byrne
Journal:  J Gen Microbiol       Date:  1981-01

5.  The end products of the metabolism of aromatic amino acids by Clostridia.

Authors:  S R Elsden; M G Hilton; J M Waller
Journal:  Arch Microbiol       Date:  1976-04-01       Impact factor: 2.552

6.  Selective and differential medium for isolation of Clostridium difficile.

Authors:  W L George; V L Sutter; D Citron; S M Finegold
Journal:  J Clin Microbiol       Date:  1979-02       Impact factor: 5.948

7.  Cultures for Clostridium difficile in stools containing a cytotoxin neutralized by Clostridium sordellii antitoxin.

Authors:  S H Willey; J G Bartlett
Journal:  J Clin Microbiol       Date:  1979-12       Impact factor: 5.948

8.  Rapid detection and presumptive identification of Clostridium difficile by p-cresol production on a selective medium.

Authors:  K D Phillips; P A Rogers
Journal:  J Clin Pathol       Date:  1981-06       Impact factor: 3.411

9.  Rapid identification of Clostridium difficile by toxin detection.

Authors:  T W Chang; S L Gorbach
Journal:  J Clin Microbiol       Date:  1982-03       Impact factor: 5.948

10.  Norleucine-tyrosine broth for rapid identification of Clostridium difficile by gas-liquid chromatography.

Authors:  O L Nunez-Montiel; F S Thompson; V R Dowell
Journal:  J Clin Microbiol       Date:  1983-02       Impact factor: 5.948

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  11 in total

1.  Rapid identification of Clostridium difficile by determination of leucine arylamidase activity.

Authors:  A Kudhair; M Delmée; G Wauters
Journal:  Eur J Clin Microbiol       Date:  1986-04       Impact factor: 3.267

Review 2.  Clostridium difficile: its disease and toxins.

Authors:  D M Lyerly; H C Krivan; T D Wilkins
Journal:  Clin Microbiol Rev       Date:  1988-01       Impact factor: 26.132

3.  Presumptive identification of Clostridium difficile by detection of p-cresol in prepared peptone yeast glucose broth supplemented with p-hydroxyphenylacetic acid.

Authors:  G Sivsammye; H V Sims
Journal:  J Clin Microbiol       Date:  1990-08       Impact factor: 5.948

4.  Identification of Clostridium difficile using the API ZYM system.

Authors:  P N Levett
Journal:  Eur J Clin Microbiol       Date:  1985-10       Impact factor: 3.267

5.  Analysis by gas liquid chromatography of production of volatile fatty acids by anaerobic bacteria grown on solid medium.

Authors:  R J Wiggins; M Wilks; S Tabaqchali
Journal:  J Clin Pathol       Date:  1985-08       Impact factor: 3.411

Review 6.  Clostridium difficile: clinical disease and diagnosis.

Authors:  F C Knoop; M Owens; I C Crocker
Journal:  Clin Microbiol Rev       Date:  1993-07       Impact factor: 26.132

7.  New rapid identification test for Clostridium difficile.

Authors:  S T Aspinall; S F Dealler
Journal:  J Clin Pathol       Date:  1992-11       Impact factor: 3.411

Review 8.  Laboratory diagnosis of Clostridium difficile-associated diarrhoea.

Authors:  R A Bowman; T V Riley
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1988-08       Impact factor: 3.267

9.  Expansion of urease- and uricase-containing, indole- and p-cresol-forming and contraction of short-chain fatty acid-producing intestinal microbiota in ESRD.

Authors:  Jakk Wong; Yvette M Piceno; Todd Z DeSantis; Madeleine Pahl; Gary L Andersen; Nosratola D Vaziri
Journal:  Am J Nephrol       Date:  2014-03-08       Impact factor: 3.754

10.  Faecal volatile biomarkers of Clostridium difficile infection.

Authors:  Mitesh Patel; Dawn Fowler; Jeremy Sizer; Christopher Walton
Journal:  PLoS One       Date:  2019-04-15       Impact factor: 3.240

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