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Literature DB >> 4065138

Identification of Clostridium difficile using the API ZYM system.

Abstract

The use of the API ZYM system for the identification of Clostridium difficile was investigated. The enzyme profiles generated by this system readily distinguished strains of Clostridium difficile from other clostridia commonly isolated from faeces. Enzyme activity of Clostridium difficile was influenced by the composition of the culture medium but appeared to be independent of the age of the culture. Given careful standardisation of techniques the API ZYM system is a suitable alternative to conventional techniques for identification of Clostridium difficile.

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Year: 1985 PMID： 4065138 DOI： 10.1007/BF02014434

Source DB: PubMed Journal: Eur J Clin Microbiol ISSN： 0722-2211 Impact factor: 3.267

11 in total

1. Evaluation of a test kit for identification of anaerobic bacteria.

Authors: A Dahlbäck; T Wadström
Journal: Med Microbiol Immunol Date: 1975-09-19 Impact factor: 3.402

2. API and Minitek systems in identification of clinical isolates of anaerobic gram-negative bacilli and Clostridium species.

Authors: C W Hanson; R Cassorla; W J Martin
Journal: J Clin Microbiol Date: 1979-07 Impact factor: 5.948

6. Comparison of API ZYM system with API AN-Ident, API 20A, Minitek Anaerobe II, and RapID-ANA systems for identification of Clostridium difficile.

Authors: C B Head; S Ratnam
Journal: J Clin Microbiol Date: 1988-01 Impact factor: 5.948

7. New rapid identification test for Clostridium difficile.

Authors: S T Aspinall; S F Dealler
Journal: J Clin Pathol Date: 1992-11 Impact factor: 3.411

7 in total

Identification of Clostridium difficile using the API ZYM system.

1. Evaluation of a test kit for identification of anaerobic bacteria.

2. API and Minitek systems in identification of clinical isolates of anaerobic gram-negative bacilli and Clostridium species.

3. The API ZYM system in the identification of Gram-negative anaerobes.

4. API ZYM: a simple rapid system for the detection of bacterial enzymes.

5. Rapid enzymatic characterization of clinically encountered anaerobic bacteria with the API ZYM system.

6. Simple method for isolation and presumptive identification of Clostridium difficile.

7. Selective and differential medium for isolation of Clostridium difficile.

8. Cultures for Clostridium difficile in stools containing a cytotoxin neutralized by Clostridium sordellii antitoxin.

9. Rapid detection and presumptive identification of Clostridium difficile by p-cresol production on a selective medium.

10. Enzymatic characterization of some oral and nonoral gram-negative bacteria with the API ZYM system.

Review 1. Clostridium difficile: its disease and toxins.

2. Clinical evaluation of the Vitek ANI card for identification of anaerobic bacteria.

3. Typing of Clostridium difficile causing diarrhoea in an orthopaedic ward.

4. Improvement of Clostridium difficile isolation by heat-shock and typing of the isolated strains by SDS-PAGE.

5. New selective medium for isolating Clostridium difficile from faeces.

6. Comparison of API ZYM system with API AN-Ident, API 20A, Minitek Anaerobe II, and RapID-ANA systems for identification of Clostridium difficile.

7. New rapid identification test for Clostridium difficile.