Literature DB >> 3963359

Purification of small oligonucleotides by polyacrylamide gel electrophoresis and transfer to diethylaminoethyl paper.

A V Vorndam, J Kerschner.   

Abstract

The application of polyacrylamide gel electrophoresis with subsequent electroelution onto DEAE paper for the purification of small oligonucleotides is described. We demonstrate that synthetic DNAs and hydrolyzed RNAs as small as three nucleotides in length can be purified by this technique. The product is undegraded and homogeneous in length.

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Year:  1986        PMID: 3963359     DOI: 10.1016/0003-2697(86)90401-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  6 in total

1.  Kunitz trypsin inhibitor genes are differentially expressed during the soybean life cycle and in transformed tobacco plants.

Authors:  K D Jofuku; R B Goldberg
Journal:  Plant Cell       Date:  1989-11       Impact factor: 11.277

2.  Organization of multiple regulatory elements in the control region of the adenovirus type 2-specific VARNA1 gene: fine mapping with linker-scanning mutants.

Authors:  J F Railey; G J Wu
Journal:  Mol Cell Biol       Date:  1988-03       Impact factor: 4.272

3.  A rapid and cost effective method in purifying small RNA.

Authors:  Marimuthu Citartan; Soo-Choon Tan; Thean-Hock Tang
Journal:  World J Microbiol Biotechnol       Date:  2011-06-03       Impact factor: 3.312

4.  RecJ 5' Exonuclease Digestion of Oligonucleotide Failure Strands: A "Green" Method of Trityl-On Purification.

Authors:  Michael Jensen; Ronald Davis
Journal:  Biochemistry       Date:  2017-05-01       Impact factor: 3.162

Review 5.  Principles and methods for the analysis and purification of synthetic deoxyribonucleotides by high-performance liquid chromatography.

Authors:  W J Warren; G Vella
Journal:  Mol Biotechnol       Date:  1995-10       Impact factor: 2.695

6.  Functional analysis of coordinated cleavage in V(D)J recombination.

Authors:  D R Kim; M A Oettinger
Journal:  Mol Cell Biol       Date:  1998-08       Impact factor: 4.272

  6 in total

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