Literature DB >> 3947381

Molecular pharmacology of manoalide. Inactivation of bee venom phospholipase A2.

K B Glaser, R S Jacobs.   

Abstract

The marine natural product manoalide (MLD) was shown to directly inactivate bee venom phospholipase A2 (PLA2). Inactivation was pH dependent (maximum inactivation occurred at pH 8.0), time dependent and concentration dependent. The IC50 was estimated at 0.05 microM and virtually complete inactivation of the enzyme occurred at 4.0 microM. The time-dependent loss of PLA2 activity suggested that inactivation does not follow typical Michaelis-Menten kinetics. Reversibility was studied directly by dilution and dialysis; both methods were ineffective in dissociating the MLD-PLA2 complex. A kinetic plot of initial velocity (v) versus [PLA2] supported our hypothesis that MLD apparently inactivates bee venom PLA2 by an irreversible mechanism.

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Year:  1986        PMID： 3947381     DOI： 10.1016/0006-2952(86)90218-2

Source DB:  PubMed          Journal:  Biochem Pharmacol        ISSN： 0006-2952            Impact factor:   5.858


  14 in total

1.  The inhibition of release of endothelium-derived relaxant factor by manoalide, a potent inhibitor of phospholipase A2.

Authors:  C J Long; H M Sarau; B A Berkowitz
Journal:  Br J Pharmacol       Date:  1987-12       Impact factor: 8.739

Review 2.  Pharmacological inhibition of leukotriene actions.

Authors:  F Engels; F P Nijkamp
Journal:  Pharm World Sci       Date:  1998-04

Review 3.  Phospholipase A2 enzymes: physical structure, biological function, disease implication, chemical inhibition, and therapeutic intervention.

Authors:  Edward A Dennis; Jian Cao; Yuan-Hao Hsu; Victoria Magrioti; George Kokotos
Journal:  Chem Rev       Date:  2011-09-12       Impact factor: 60.622

4.  Variabines A and B: new β-carboline alkaloids from the marine sponge Luffariella variabilis.

Authors:  Eriko Sakai; Hikaru Kato; Henki Rotinsulu; Fitje Losung; Remy E P Mangindaan; Nicole J de Voogd; Hideyoshi Yokosawa; Sachiko Tsukamoto
Journal:  J Nat Med       Date:  2013-05-18       Impact factor: 2.343

5.  The role of polyphosphoinositides and their breakdown products in A23187-induced release of arachidonic acid from rabbit polymorphonuclear leucocytes.

Authors:  C J Meade; G A Turner; P E Bateman
Journal:  Biochem J       Date:  1986-09-01       Impact factor: 3.857

6.  Chemical modification and inactivation of phospholipases A2 by a manoalide analogue.

Authors:  S Fujii; Y Tahara; M Toyomoto; S Hada; H Nishimura; S Inoue; K Ikeda; Y Inagaki; S Katsumura; Y Samejima
Journal:  Biochem J       Date:  1995-05-15       Impact factor: 3.857

Review 7.  Bioactive sesterterpenes and triterpenes from marine sponges: occurrence and pharmacological significance.

Authors:  Sherif S Ebada; Wenhan Lin; Peter Proksch
Journal:  Mar Drugs       Date:  2010-02-23       Impact factor: 5.118

8.  The antiinflammatory activity of topically applied novel calcium-channel antagonists.

Authors:  G W De Vries; A McLaughlin; M B Wenzel; J Perez; D Harcourt; G Lee; M Garst; L A Wheeler
Journal:  Inflammation       Date:  1995-04       Impact factor: 4.092

9.  Structure and biosynthesis of novel conjugated polyene fatty acids from the marine green alga Anadyomene stellata.

Authors:  M V Mikhailova; D L Bemis; M L Wise; W H Gerwick; J N Norris; R S Jacobs
Journal:  Lipids       Date:  1995-07       Impact factor: 1.880

10.  Effect of manoalide on human 5-lipoxygenase activity.

Authors:  F Cabré; A Carabaza; N Suesa; A M García; E Rotllan; M Gómez; D Tost; D Mauleón; G Carganico
Journal:  Inflamm Res       Date:  1996-05       Impact factor: 4.575
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