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Literature DB >> 3937726

Sequence analysis of non-expressed immunoglobulin heavy chain loci in clonally related, somatically mutated hybridoma cells.

Abstract

The non-expressed, rearranged JH loci of two hybridoma cells, thought to be derived from a single B cell precursor were cloned and partially sequenced. Identical DJ rearrangements including N sequences at the DJ border were found, proving the common origin of the cells. The non-expressed loci lacked a rearranged VH gene and exhibited a single point mutation in a stretch of 1108 bp. This contrasts with 11 somatic point mutations in the 702 bp of the expressed VDJ regions. We discuss the possibility that the mechanism of somatic hypermutation may require V gene rearrangement.

Entities: Chemical

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Year: 1985 PMID： 3937726 PMCID： PMC554681 DOI： 10.1002/j.1460-2075.1985.tb04101.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

17 in total

1. Continuous cultures of fused cells secreting antibody of predefined specificity.

Authors: G Köhler; C Milstein
Journal: Nature Date: 1975-08-07 Impact factor: 49.962

2. Packaging recombinant DNA molecules into bacteriophage particles in vitro.

Authors: B Hohn; K Murray
Journal: Proc Natl Acad Sci U S A Date: 1977-08 Impact factor: 11.205

3. Analysis of the repertoire of anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies in C 57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies.

Authors: M Reth; T Imanishi-Kari; K Rajewsky
Journal: Eur J Immunol Date: 1979-12 Impact factor: 5.532

4. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.

Authors: F Sanger; A R Coulson; B G Barrell; A J Smith; B A Roe
Journal: J Mol Biol Date: 1980-10-25 Impact factor: 5.469

5. Differences between germ-line and rearranged immunoglobulin V kappa coding sequences suggest a localized mutation mechanism.

Authors: M Pech; J Höchtl; H Schnell; H G Zachau
Journal: Nature Date: 1981-06-25 Impact factor: 49.962

6. Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions.

Authors: F W Alt; D Baltimore
Journal: Proc Natl Acad Sci U S A Date: 1982-07 Impact factor: 11.205

7. Sequences of the joining region genes for immunoglobulin heavy chains and their role in generation of antibody diversity.

Authors: N M Gough; O Bernard
Journal: Proc Natl Acad Sci U S A Date: 1981-01 Impact factor: 11.205

6. In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. III. The kinetics of V region mutation and selection in germinal center B cells.

Authors: J Jacob; J Przylepa; C Miller; G Kelsoe
Journal: J Exp Med Date: 1993-10-01 Impact factor: 14.307

6 in total

Sequence analysis of non-expressed immunoglobulin heavy chain loci in clonally related, somatically mutated hybridoma cells.

1. Continuous cultures of fused cells secreting antibody of predefined specificity.

2. Packaging recombinant DNA molecules into bacteriophage particles in vitro.

3. Analysis of the repertoire of anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies in C 57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies.

4. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.

5. Differences between germ-line and rearranged immunoglobulin V kappa coding sequences suggest a localized mutation mechanism.

6. Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions.

7. Sequences of the joining region genes for immunoglobulin heavy chains and their role in generation of antibody diversity.

8. Somatic mutations of immunoglobulin variable genes are restricted to the rearranged V gene.

9. Molecular basis of an isogeneic anti-idiotypic response.

10. Organization, structure, and assembly of immunoglobulin heavy chain diversity DNA segments.

1. Stepwise intraclonal maturation of antibody affinity through somatic hypermutation.

2. Patterns of somatic mutations in immunoglobulin variable genes.

3. Clonal recruitment and somatic mutation in the generation of immunological memory to the hapten NP.

4. Somatic hypermutation of an immunoglobulin mu heavy chain transgene.

5. Analysis of somatic mutations in kappa transgenes.

6. In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. III. The kinetics of V region mutation and selection in germinal center B cells.