Literature DB >> 3937490

Purification and characterization of an extracellular glucoamylase from the yeast Candida tsukubaensis CBS 6389.

R De Mot, E Van Oudendijck, H Verachtert.   

Abstract

The starch-degrading yeast Candida tsukubaensis CBS 6389 secreted amylase at high activity when grown in a medium containing soluble starch. The extracellular alpha-amylase activity was very low. The major amylase component was purified by DEAE-Sephadex A-50 chromatography and Ultrogel AcA 44 gel filtration and characterized as a glucoamylase. The enzyme proved to be a glycoprotein with a molecular weight of 56 000. The glucoamylase had a temperature optimum at 55 degrees C and displayed highest activity in a pH range of 2.4-4.8. Acarbose strongly inhibited the purified glucoamylase. Debranching activity was present as demonstrated by the release of glucose from pullulan.

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Year:  1985        PMID： 3937490     DOI： 10.1007/bf02439937

Source DB:  PubMed          Journal:  Antonie Van Leeuwenhoek        ISSN： 0003-6072            Impact factor:   2.271


  9 in total

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Authors:  P Manjunath; B C Shenoy; M R Raghavendra Rao
Journal:  J Appl Biochem       Date:  1983 Aug-Oct

9.  Isolation and characterization of Schwanniomyces alluvius amylolytic enzymes.

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Journal:  Appl Environ Microbiol       Date:  1982-08       Impact factor: 4.792
  9 in total
  5 in total

1.  Mycocin production in Pseudozyma tsukubaensis.

Authors:  Wladyslav I Golubev; Ilona Pfeiffer; Ellen W Golubeva
Journal:  Mycopathologia       Date:  2006-10       Impact factor: 2.574

2.  Purification and Characterization of Extracellular Amylolytic Enzymes from the Yeast Filobasidium capsuligenum.

Authors:  R De Mot; H Verachtert
Journal:  Appl Environ Microbiol       Date:  1985-12       Impact factor: 4.792

3.  Localization and Characterization of alpha-Glucosidase Activity in Brettanomyces lambicus.

Authors:  H M Kumara; S De Cort; H Verachtert
Journal:  Appl Environ Microbiol       Date:  1993-08       Impact factor: 4.792

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Authors:  P R Williamson; M A Huber; J E Bennett
Journal:  Biochem J       Date:  1993-05-01       Impact factor: 3.857

5.  Molecular cloning and characterization of a Candida tsukubaensis alpha-glucosidase gene in the yeast Saccharomyces cerevisiae.

Authors:  B T Kinsella; A Larkin; M Bolton; B A Cantwell
Journal:  Curr Genet       Date:  1991-07       Impact factor: 3.886
  5 in total

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