Moderate changes in linoleate intake do not influence the systemic production of E prostaglandins.

A pilot study was undertaken to determine if moderate changes in linoleate (18:2 omega 6) intake would modulate the prostaglandin E turnover concurrently with, or independently of, changes in the plasma prostaglandin (PG) precursor levels. Four adult male volunteers in good health were fed two controlled diets containing 35% of energy from fat, with either 10 (diet L) or 30 g (diet H) linoleate/day, 30 to 50 g saturated fatty acids/day, and the balance mainly monounsaturated fatty acids. All four subjects were consuming sufficient amounts of polyunsaturates before the study. Protein (13-14%) and carbohydrate (51-53%) contribution to total caloric intake was kept constant. The menu cycle was 7 days, and all diets were calculated to provide adequate amounts of nutrients known to be required by man when data were available. Plasma fatty acids were determined by gas-liquid chromatography, and the turnover of E prostaglandins was assessed by measuring the urinary output of the major metabolite of PGE1 + PGE2 (PGE-M). Whereas we found a clear correlation between 18:2 omega 6 intake and 18:2 omega 6 concentrations in the neutral lipid (P = 0.007) and phosphoglyceride (P = 0.012) fractions of plasma, arachidonate (20:4 omega 6) concentrations in those same plasma fractions did not respond significantly to changes in linoleate intake. Moreover, we could not detect an influence of moderate changes in dietary levels of 18:2 omega 6 on the systemic production of PGE as measured by the daily urinary output of PGE-M.