Literature DB >> 392508

Detection of proteins like human gamma and beta globins in Escherichia coli carrying recombinant DNA plasmids.

L B Wilson, H E Grenett, D M Doran, F A Garver, J T Wilson.   

Abstract

Escherichia coli strain chi 1776 carrying recombinant DNA plasmids containing cDNA copies of human beta or gamma globin mRNAs has been shown by radioimmunoassay to synthesize polypeptides antigenically related to the beta and gamma chains of human hemoglobin. The gamma and beta polypeptides have been enriched from lysates on immunoabsorbent columns containing hemoglobin antibodies and shown to specifically inhibit the antigen-antibody binding between 125I-labeled hemoglobin and the homologous antibody but not other hemoglobin-antihemoglobin reactions. Clone JW151, which is known to contain a complete copy of the coding portion of the gamma globin mRNA, has been shown to produce a protein that reacts specifically with antibody to the chain of fetal hemoglobin, hemoglobin Kenya, and hemoglobin Bart's.

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Year:  1979        PMID： 392508      PMCID： PMC411703          DOI： 10.1073/pnas.76.11.5631

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

1.  A bacterial clone synthesizing proinsulin.

Authors:  L Villa-Komaroff; A Efstratiadis; S Broome; P Lomedico; R Tizard; S P Naber; W L Chick; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

2.  Functional expression of cloned yeast DNA in Escherichia coli: specific complementation of argininosuccinate lyase (argH) mutations.

Authors:  L Clarke; J Carbon
Journal:  J Mol Biol       Date:  1978-04-25       Impact factor: 5.469

3.  Expression in Escherichia coli of chemically synthesized genes for human insulin.

Authors:  D V Goeddel; D G Kleid; F Bolivar; H L Heyneker; D G Yansura; R Crea; T Hirose; A Kraszewski; K Itakura; A D Riggs
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

4.  Radioimmunochemical characterization of hemoglobins Lepore and Kenya: unique antigenic determinants located on hybrid hemoglobins.

Authors:  F A Garver; G Altay; M M Baker; M Gravely; T H Huisman
Journal:  J Immunol       Date:  1978-10       Impact factor: 5.422

5.  Amplification and characterization of a beta-globin gene synthesized in vitro.

Authors:  T Maniatis; S G Kee; A Efstratiadis; F C Kafatos
Journal:  Cell       Date:  1976-06       Impact factor: 41.582

6.  Insertion of synthetic copies of human globin genes into bacterial plasmids.

Authors:  J T Wilson; L B Wilson; J K deRiel; L Villa-komaroff; A Efstratiadis; B G Forget; S M Weissman
Journal:  Nucleic Acids Res       Date:  1978-02       Impact factor: 16.971

7.  Protein expression in E. coli minicells by recombinant plasmids.

Authors:  R B Meagher; R C Tait; M Betlach; H W Boyer
Journal:  Cell       Date:  1977-03       Impact factor: 41.582

8.  Synthesis of an ovalbumin-like protein by Escherichia coli K12 harbouring a recombinant plasmid.

Authors:  O Mercereau-Puijalon; A Royal; B Cami; A Garapin; A Krust; F Gannon; P Kourilsky
Journal:  Nature       Date:  1978-10-12       Impact factor: 49.962

9.  Translation of Drosophila melanogaster sequences in Escherichia coli.

Authors:  A Rambach; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

10.  Human globin messenger RNA: importance of cloning for structural analysis.

Authors:  J T Wilson; B G Forget; L B Wilson; S M Weissman
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728
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  2 in total

1.  Efficient cell proliferation and predominant accumulation of epsilon-globin mRNA in human leukemic K562 cells which produce mostly Hb Gower 1.

Authors:  R Gambari; F Amelotti; R Piva
Journal:  Experientia       Date:  1985-05-15

2.  Restriction endonuclease mapping of gamma-delta-beta-globin region in G gamma (beta)+ HPFH and a Chinese A gamma HPFH variant.

Authors:  M Farquhar; R Gelinas; B Tatsis; J Murray; M Yagi; R Mueller; G Stamatoyannopoulos
Journal:  Am J Hum Genet       Date:  1983-07       Impact factor: 11.025
  2 in total

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