Literature DB >> 3923155

Identification of caprine arthritis-encephalitis retrovirus proteins in immunodiffusion precipitin lines.

D S Adams, R P Gogolewski, A F Barbet, W P Cheevers.   

Abstract

Precipitin lines formed between serum from a goat infected with caprine arthritis-encephalitis virus (CAEV) and radiolabelled viral proteins in polyethylene glycol-concentrated culture medium were excised from immunodiffusion (ID) plates and analysed by polyacrylamide gel electrophoresis. The two major precipitin lines contained the 135 000 mol. wt. glycoprotein (gp 135) and the internal 28 000 mol. wt. structural protein (p28). This method obviates the use of purified proteins or monospecific antisera to positively determine viral constituents in ID precipitin lines formed between a crude antigen preparation and antiserum against whole virus.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 3923155     DOI： 10.1099/0022-1317-66-5-1139

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  5 in total

1.  Detection of caprine arthritis-encephalitis- and maedi-visna viruses using the polymerase chain reaction.

Authors:  R Zanoni; U Pauli; E Peterhans
Journal:  Experientia       Date:  1990-03-15

2.  Detection of antibodies to caprine arthritis-encephalitis virus by protein G enzyme-linked immunosorbent assay and immunoblotting.

Authors:  R Zanoni; A Krieg; E Peterhans
Journal:  J Clin Microbiol       Date:  1989-03       Impact factor: 5.948

3.  Structure and genetic variability of envelope glycoproteins of two antigenic variants of caprine arthritis-encephalitis lentivirus.

Authors:  D P Knowles; W P Cheevers; T C McGuire; A L Brassfield; W G Harwood; T A Stem
Journal:  J Virol       Date:  1991-11       Impact factor: 5.103

4.  Expression in Escherichia coli and sequencing of the coding region for the capsid protein of Dutch maedi-visna virus strain ZZV 1050: application of recombinant protein in enzyme-linked immunosorbent assay for the detection of caprine and ovine lentiviruses.

Authors:  R G Zanoni; I M Nauta; U Pauli; E Peterhans
Journal:  J Clin Microbiol       Date:  1991-07       Impact factor: 5.948

5.  Transmembrane protein oligomers of caprine arthritis-encephalitis lentivirus are immunodominant in goats with progressive arthritis.

Authors:  T C McGuire; D P Knowles; W C Davis; A L Brassfield; T A Stem; W P Cheevers
Journal:  J Virol       Date:  1992-05       Impact factor: 5.103
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.