Literature DB >> 2155815

Detection of caprine arthritis-encephalitis- and maedi-visna viruses using the polymerase chain reaction.

R Zanoni1, U Pauli, E Peterhans.   

Abstract

The polymerase chain reaction (PCR) was used to demonstrate proviral DNA of lentiviruses of small ruminants in cultured cells. Primers for the Taq polymerase were selected in the GAG gene of Icelandic maedi-visna virus and POL gene of caprine arthritis-encephalitis (CAE) virus. Using PCR, proviral DNA of CAE virus was detected at 1 day post infection, 4 days before viral protein could be demonstrated using a sensitive immunoblotting protocol and 6 days before the appearance of syncytia. Primers derived from the published sequence of CAE virus successfully primed for the synthesis of homologous virus and Icelandic maedi-visna virus but not for maedi-visna virus isolated in The Netherlands. In contrast, primers derived from the GAG region of Icelandic maedi-visna virus allowed the amplification of DNA of homologous virus, maedi-visna virus isolated in The Netherlands as well as CAE virus.

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Year:  1990        PMID： 2155815     DOI： 10.1007/BF01951776

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  21 in total

1.  Nucleotide sequence of the visna lentivirus: relationship to the AIDS virus.

Authors:  P Sonigo; M Alizon; K Staskus; D Klatzmann; S Cole; O Danos; E Retzel; P Tiollais; A Haase; S Wain-Hobson
Journal:  Cell       Date:  1985-08       Impact factor: 41.582

2.  Identification of HIV-infected seronegative individuals by a direct diagnostic test based on hybridisation to amplified viral DNA.

Authors:  M Loche; B Mach
Journal:  Lancet       Date:  1988-08-20       Impact factor: 79.321

3.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Sequence homology between cloned caprine arthritis encephalitis virus and visna virus, two neurotropic lentiviruses.

Authors:  J M Pyper; J E Clements; M A Gonda; O Narayan
Journal:  J Virol       Date:  1986-05       Impact factor: 5.103

6.  An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to maedi-visna virus.

Authors:  D J Houwers; A L Gielkens; J Schaake
Journal:  Vet Microbiol       Date:  1982-07       Impact factor: 3.293

7.  Identification of caprine arthritis-encephalitis retrovirus proteins in immunodiffusion precipitin lines.

Authors:  D S Adams; R P Gogolewski; A F Barbet; W P Cheevers
Journal:  J Gen Virol       Date:  1985-05       Impact factor: 3.891

8.  Rates and dates of divergence between AIDS virus nucleotide sequences.

Authors:  W H Li; M Tanimura; P M Sharp
Journal:  Mol Biol Evol       Date:  1988-07       Impact factor: 16.240

9.  Molecular cloning of integrated caprine arthritis-encephalitis virus.

Authors:  A Yaniv; J E Dahlberg; S R Tronick; I M Chiu; S A Aaronson
Journal:  Virology       Date:  1985-09       Impact factor: 3.616

10.  Nucleotide sequence evidence for relationship of AIDS retrovirus to lentiviruses.

Authors:  I M Chiu; A Yaniv; J E Dahlberg; A Gazit; S F Skuntz; S R Tronick; S A Aaronson
Journal:  Nature       Date:  1985 Sep 26-Oct 2       Impact factor: 49.962
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  7 in total

1.  Host-virus interaction as defined by amplification of viral DNA and serology in lentivirus-infected sheep.

Authors:  S J Brodie; L D Pearson; G D Snowder; J C DeMartini
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

2.  Expression in Escherichia coli and sequencing of the coding region for the capsid protein of Dutch maedi-visna virus strain ZZV 1050: application of recombinant protein in enzyme-linked immunosorbent assay for the detection of caprine and ovine lentiviruses.

Authors:  R G Zanoni; I M Nauta; U Pauli; E Peterhans
Journal:  J Clin Microbiol       Date:  1991-07       Impact factor: 5.948

3.  Rapid and biologically safe diagnosis of African swine fever virus infection by using polymerase chain reaction.

Authors:  Y Steiger; M Ackermann; C Mettraux; U Kihm
Journal:  J Clin Microbiol       Date:  1992-01       Impact factor: 5.948

4.  Evaluation of Serological Methods and a New Real-Time Nested PCR for Small Ruminant Lentiviruses.

Authors:  Jessica Schaer; Zeljko Cvetnic; Tomislav Sukalic; Sven Dörig; Martin Grisiger; Carmen Iscaro; Francesco Feliziani; Folke Pfeifer; Francesco Origgi; Reto Giacomo Zanoni; Carlos Eduardo Abril
Journal:  Pathogens       Date:  2022-01-21

Review 5.  Small ruminant lentiviruses: genetic variability, tropism and diagnosis.

Authors:  Hugo Ramírez; Ramsés Reina; Beatriz Amorena; Damián de Andrés; Humberto A Martínez
Journal:  Viruses       Date:  2013-04-23       Impact factor: 5.048

Review 6.  Expanding possibilities for intervention against small ruminant lentiviruses through genetic marker-assisted selective breeding.

Authors:  Stephen N White; Donald P Knowles
Journal:  Viruses       Date:  2013-06-14       Impact factor: 5.048

Review 7.  Serological, Molecular and Culture-Based Diagnosis of Lentiviral Infections in Small Ruminants.

Authors:  Aphrodite I Kalogianni; Ioannis Stavropoulos; Serafeim C Chaintoutis; Ioannis Bossis; Athanasios I Gelasakis
Journal:  Viruses       Date:  2021-08-27       Impact factor: 5.048
  7 in total

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