Literature DB >> 3921826

Independent immunoglobulin class-switch events occurring in a single myeloma cell line.

L A Eckhardt, B K Birshtein.   

Abstract

Five gamma 2a-producing cell lines, all derived from the gamma 2b-producing mouse myeloma MPC11, were analyzed for changes in gene structure. Also examined was a cell line (ICR9.7.1) that acts as an intermediate to some of these class switches. All six of the MPC11 variants have undergone DNA rearrangement. Rearrangement sites in the gamma 2a-producing cells are different in each case and generally do not involve tandem repeat sequences. An enhancer for heavy chain gene transcription was deleted in at least one of these cell lines as a result of its class-switch rearrangement. DNA sequence analysis of cloned genes from two of the MPC11 variants revealed shared sequences at their rearrangement breakpoints. The same sequences are found at the breakpoints of two additional immunoglobulin gene rearrangements in MPC11.

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Year:  1985        PMID： 3921826      PMCID： PMC366791          DOI： 10.1128/mcb.5.4.856-868.1985

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  42 in total

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Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  Mutations in immunoglobulin-producing mouse myeloma cells.

Authors:  R Baumal; B K Birshtein; P Coffino; M D Scharff
Journal:  Science       Date:  1973-10-12       Impact factor: 47.728

4.  Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.

Authors:  F R Blattner; B G Williams; A E Blechl; K Denniston-Thompson; H E Faber; L Furlong; D J Grunwald; D O Kiefer; D D Moore; J W Schumm; E L Sheldon; O Smithies
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

5.  Primary and secondary variants in immunoglobulin heavy chain production.

Authors:  S Koskimies; B K Birshtein
Journal:  Nature       Date:  1976-12-02       Impact factor: 49.962

6.  Variants of a mouse myeloma cell line that synthesize immunoglobulin heavy chains having an altered serotype.

Authors:  J L Preud'Homme; B K Birshtein; M D Scharff
Journal:  Proc Natl Acad Sci U S A       Date:  1975-04       Impact factor: 11.205

7.  In vitro packaging of a lambda Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1.

Authors:  N Sternberg; D Tiemeier; L Enquist
Journal:  Gene       Date:  1977-05       Impact factor: 3.688

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  Expression and regulation of immunoglobulin heavy chain gene transfected into lymphoid cells.

Authors:  M S Neuberger
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

10.  Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits.

Authors:  R Laskov; M D Scharff
Journal:  J Exp Med       Date:  1970-03-01       Impact factor: 14.307
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  32 in total

1.  Transcription of germ line V alpha segments correlates with ongoing T-cell receptor alpha-chain rearrangement.

Authors:  J D Fondell; K B Marcu
Journal:  Mol Cell Biol       Date:  1992-04       Impact factor: 4.272

2.  The importance of the 3'-enhancer region in immunoglobulin kappa gene expression.

Authors:  K B Meyer; M J Sharpe; M A Surani; M S Neuberger
Journal:  Nucleic Acids Res       Date:  1990-10-11       Impact factor: 16.971

3.  Spontaneous deletions in Ig heavy chain genes: flanking sequences influence splice site selection.

Authors:  S B Ward; S L Morrison
Journal:  Nucleic Acids Res       Date:  1991-12-11       Impact factor: 16.971

4.  Immunoglobulin heavy-chain enhancer is required to maintain transfected gamma 2A gene expression in a pre-B-cell line.

Authors:  B Porton; D M Zaller; R Lieberson; L A Eckhardt
Journal:  Mol Cell Biol       Date:  1990-03       Impact factor: 4.272

5.  Extinction of expression of immunoglobulin genes in myeloma X fibroblast somatic cell hybrids.

Authors:  A Greenberg; R Ber; Z Kra-Oz; R Laskov
Journal:  Mol Cell Biol       Date:  1987-02       Impact factor: 4.272

6.  A B-cell-specific nuclear protein that binds to DNA sites 5' to immunoglobulin S alpha tandem repeats is regulated during differentiation.

Authors:  S H Waters; K U Saikh; J Stavnezer
Journal:  Mol Cell Biol       Date:  1989-12       Impact factor: 4.272

7.  Recombination and transcription of the endogenous Ig heavy chain locus is effected by the Ig heavy chain intronic enhancer core region in the absence of the matrix attachment regions.

Authors:  E Sakai; A Bottaro; L Davidson; B P Sleckman; F W Alt
Journal:  Proc Natl Acad Sci U S A       Date:  1999-02-16       Impact factor: 11.205

8.  Transcription of a productively rearranged Ig VDJC alpha does not require the presence of HS4 in the IgH 3' regulatory region.

Authors:  Buyi Zhang; Adrienne Alaie-Petrillo; Maria Kon; Fubin Li; Laurel A Eckhardt
Journal:  J Immunol       Date:  2007-05-15       Impact factor: 5.422

9.  NF-HB (BSAP) is a repressor of the murine immunoglobulin heavy-chain 3' alpha enhancer at early stages of B-cell differentiation.

Authors:  M Singh; B K Birshtein
Journal:  Mol Cell Biol       Date:  1993-06       Impact factor: 4.272

10.  Nonhomologous recombination at sites within the mouse JH-C delta locus accompanies C mu deletion and switch to immunoglobulin D secretion.

Authors:  J D Owens; F D Finkelman; J D Mountz; J F Mushinski
Journal:  Mol Cell Biol       Date:  1991-11       Impact factor: 4.272
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