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Literature DB >> 6219268

Construction and physical mapping of plasmids containing the metJBLF gene cluster of E. coli K12.

M M Zakin, R C Greene, A Dautry-Varsat, N Duchange, P Ferrara, M C Py, D Margarita, G N Cohen.

Abstract

In vitro recombination techniques were used to clone the E. coli metJBLF gene cluster in a plasmid vector. Several chimeric plasmids were obtained, analyzed by restriction mapping and characterized genetically. The combined results establish that the met gene cluster is contained on an approximately 5.6 kilobase segment of bacterial DNA with metL between metB and metF. The origin of metL was localized precisely by its DNA sequence and its transcription direction was established.

Entities: Gene Species

Mesh：

Substances：

Year: 1982 PMID： 6219268 DOI： 10.1007/bf00384390

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

30 in total

1. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors: T Maniatis; A Jeffrey; H van deSande
Journal: Biochemistry Date: 1975-08-26 Impact factor: 3.162

2. Detection of the homology among proteins by immunochemical cross-reactivity between denatured antigens. Application to the threonine and methionine regulated aspartokinases-homoserine dehydrogenases from Escherichia coli K 12.

Authors: M M Zakin; J R Garel; A Dautry-Varsat; G N Cohen; G Boulot
Journal: Biochemistry Date: 1978-10-03 Impact factor: 3.162

3. Subunit structure of the methionine-repressible aspartokinase II--homoserine dehydrogenase II from Escherichia coli K12.

Authors: A Dautry-Varsat; L Sibilli-Weill; G N Cohen
Journal: Eur J Biochem Date: 1977-06-01

4. Large scale purification of a bacterial gene.

Authors: P Tiollais; A Rambach; H Buc
Journal: FEBS Lett Date: 1974-11-01 Impact factor: 4.124

5. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors: D B Clewell
Journal: J Bacteriol Date: 1972-05 Impact factor: 3.490

6. Construction and expression of a hybrid plasmid containing the Escherichia coli thrA and thrB genes.

Authors: P Cossart; M Katinka; M Yaniv; I Saint Girons; G N Cohen
Journal: Mol Gen Genet Date: 1979-08

Review 7. Linkage map of Escherichia coli K-12, edition 6.

Authors: B J Bachmann; K B Low
Journal: Microbiol Rev Date: 1980-03

8. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.

Authors: A C Peacock; C W Dingman
Journal: Biochemistry Date: 1967-06 Impact factor: 3.162

9. Regulation of methionine biosynthesis in Escherichia coli: mapping of the metJ locus and properties of a metJ plus-metJ minus diploid.

Authors: C H Su; R C Greene
Journal: Proc Natl Acad Sci U S A Date: 1971-02 Impact factor: 11.205

9. Nucleotide sequence of metF, the E. coli structural gene for 5-10 methylene tetrahydrofolate reductase and of its control region.

Authors: I Saint-Girons; N Duchange; M M Zakin; I Park; D Margarita; P Ferrara; G N Cohen
Journal: Nucleic Acids Res Date: 1983-10-11 Impact factor: 16.971

9 in total

Construction and physical mapping of plasmids containing the metJBLF gene cluster of E. coli K12.

1. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

2. Detection of the homology among proteins by immunochemical cross-reactivity between denatured antigens. Application to the threonine and methionine regulated aspartokinases-homoserine dehydrogenases from Escherichia coli K 12.

3. Subunit structure of the methionine-repressible aspartokinase II--homoserine dehydrogenase II from Escherichia coli K12.

4. Large scale purification of a bacterial gene.

5. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

6. Construction and expression of a hybrid plasmid containing the Escherichia coli thrA and thrB genes.

Review 7. Linkage map of Escherichia coli K-12, edition 6.

8. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.

9. Regulation of methionine biosynthesis in Escherichia coli: mapping of the metJ locus and properties of a metJ plus-metJ minus diploid.

10. The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA.

Review 1. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

2. Nucleotide sequence of katG, encoding catalase HPI of Escherichia coli.

3. Operator-constitutive mutations of the Escherichia coli metF gene.

4. Insertion mutagenesis of the metJBLF gene cluster of Escherichia coli K-12: evidence for an metBL operon.

Review 5. Linkage map of Escherichia coli K-12, edition 7.

6. Mutagenesis of the metJBLF gene cluster with transposon Tn5: localization of the metF transcription unit.

7. Physical organization of the metJB component of the Escherichia coli K-12 metJBLF gene cluster.

8. Purification and properties of NADH-dependent 5, 10-methylenetetrahydrofolate reductase (MetF) from Escherichia coli.

9. Nucleotide sequence of metF, the E. coli structural gene for 5-10 methylene tetrahydrofolate reductase and of its control region.