Literature DB >> 3900639

An improved assay for DNA ligase reveals temperature-sensitive activity in cdc9 mutants of Saccharomyces cerevisiae.

D G Barker, A L Johnson, L H Johnston.   

Abstract

A sensitive and quantitative assay for DNA ligase has been developed which is suitable for the analysis of crude cell extracts of yeast. The assay is sufficiently sensitive to detect the low levels of DNA ligase activity remaining in cdc9 mutants of Saccharomyces cerevisiae. Indeed, we have been able to show that this residual activity is temperature-sensitive, thus establishing finally that CDC9 is the structural gene for DNA ligase.

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Year:  1985        PMID： 3900639     DOI： 10.1007/bf00425731

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  11 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Saccharomyces cerevisiae cell cycle mutant cdc9 is defective in DNA ligase.

Authors:  L H Johnston; K A Nasmyth
Journal:  Nature       Date:  1978-08-31       Impact factor: 49.962

3.  Linkage of polynucleotides through phosphodiester bonds by an enzyme from Escherichia coli.

Authors:  B M Olivera; I R Lehman
Journal:  Proc Natl Acad Sci U S A       Date:  1967-05       Impact factor: 11.205

4.  Genetic Control of the Cell Division Cycle in Yeast: V. Genetic Analysis of cdc Mutants.

Authors:  L H Hartwell; R K Mortimer; J Culotti; M Culotti
Journal:  Genetics       Date:  1973-06       Impact factor: 4.562

Review 5.  DNA ligases of eukaryotes.

Authors:  S Söderhäll; T Lindahl
Journal:  FEBS Lett       Date:  1976-08-01       Impact factor: 4.124

6.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

7.  Kinetics and effect of salts and polyamines on T4 polynucleotide ligase.

Authors:  A J Raae; R K Kleppe; K Kleppe
Journal:  Eur J Biochem       Date:  1975-12-15

8.  A system for shotgun DNA sequencing.

Authors:  J Messing; R Crea; P H Seeburg
Journal:  Nucleic Acids Res       Date:  1981-01-24       Impact factor: 16.971

9.  Cloning of influenza cDNA ino M13: the sequence of the RNA segment encoding the A/PR/8/34 matrix protein.

Authors:  G Winter; S Fields
Journal:  Nucleic Acids Res       Date:  1980-05-10       Impact factor: 16.971

10.  Saccharomyces cerevisiae cdc9, a structural gene for yeast DNA ligase which complements Schizosaccharomyces pombe cdc17.

Authors:  D G Barker; L H Johnston
Journal:  Eur J Biochem       Date:  1983-08-01
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  21 in total

1.  A DNA ligase from a hyperthermophilic archaeon with unique cofactor specificity.

Authors:  M Nakatani; S Ezaki; H Atomi; T Imanaka
Journal:  J Bacteriol       Date:  2000-11       Impact factor: 3.490

2.  Mitochondrial DNA ligase III function is independent of Xrcc1.

Authors:  U Lakshmipathy; C Campbell
Journal:  Nucleic Acids Res       Date:  2000-10-15       Impact factor: 16.971

Review 3.  To shape a cell: an inquiry into the causes of morphogenesis of microorganisms.

Authors:  F M Harold
Journal:  Microbiol Rev       Date:  1990-12

4.  A novel interaction between DNA ligase III and DNA polymerase gamma plays an essential role in mitochondrial DNA stability.

Authors:  Ananya De; Colin Campbell
Journal:  Biochem J       Date:  2007-02-15       Impact factor: 3.857

5.  Cloning and functional characterization of an NAD(+)-dependent DNA ligase from Staphylococcus aureus.

Authors:  F S Kaczmarek; R P Zaniewski; T D Gootz; D E Danley; M N Mansour; M Griffor; A V Kamath; M Cronan; J Mueller; D Sun; P K Martin; B Benton; L McDowell; D Biek; M B Schmid
Journal:  J Bacteriol       Date:  2001-05       Impact factor: 3.490

6.  Effects of DNA-binding drugs on T4 DNA ligase.

Authors:  A Montecucco; G Pedrali-Noy; S Spadari; M Lestingi; G Ciarrocchi
Journal:  Biochem J       Date:  1990-03-01       Impact factor: 3.857

7.  Identification of essential residues in Thermus thermophilus DNA ligase.

Authors:  J Luo; F Barany
Journal:  Nucleic Acids Res       Date:  1996-08-01       Impact factor: 16.971

Review 8.  Yeast chromosome replication and segregation.

Authors:  C S Newlon
Journal:  Microbiol Rev       Date:  1988-12

9.  NuA4 subunit Yng2 function in intra-S-phase DNA damage response.

Authors:  John S Choy; Stephen J Kron
Journal:  Mol Cell Biol       Date:  2002-12       Impact factor: 4.272

10.  Cloning, nucleotide sequence, and engineered expression of Thermus thermophilus DNA ligase, a homolog of Escherichia coli DNA ligase.

Authors:  G Lauer; E A Rudd; D L McKay; A Ally; D Ally; K C Backman
Journal:  J Bacteriol       Date:  1991-08       Impact factor: 3.490
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