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Literature DB >> 1840584

Cloning, nucleotide sequence, and engineered expression of Thermus thermophilus DNA ligase, a homolog of Escherichia coli DNA ligase.

G Lauer¹, E A Rudd, D L McKay, A Ally, D Ally, K C Backman.

Abstract

We have cloned and sequenced the gene for DNA ligase from Thermus thermophilus. A comparison of this sequence and those of other ligases reveals significant homology only with that of Escherichia coli. The overall amino acid composition of the thermophilic ligase and the pattern of amino acid substitutions between the two proteins are consistent with compositional biases in other thermophilic enzymes. We have engineered the expression of the T. thermophilus gene in Escherichia coli, and we show that E. coli proteins may be substantially removed from the thermostable ligase by a simple heat precipitation step.

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Year: 1991 PMID： 1840584 PMCID： PMC208194 DOI： 10.1128/jb.173.16.5047-5053.1991

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

46 in total

1. Transcriptional mapping and nucleotide sequence of a vaccinia virus gene encoding a polypeptide with extensive homology to DNA ligases.

Authors: G L Smith; Y S Chan; S M Kerr
Journal: Nucleic Acids Res Date: 1989-11-25 Impact factor: 16.971

2. Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli.

Authors: Y Ishino; H Shinagawa; K Makino; S Tsunasawa; F Sakiyama; A Nakata
Journal: Mol Gen Genet Date: 1986-07

3. Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage.

Authors: B Weiss; C C Richardson
Journal: Proc Natl Acad Sci U S A Date: 1967-04 Impact factor: 11.205

4. Information content of binding sites on nucleotide sequences.

Authors: T D Schneider; G D Stormo; L Gold; A Ehrenfeucht
Journal: J Mol Biol Date: 1986-04-05 Impact factor: 5.469

5. Domain structure in yeast tRNA ligase.

Authors: Q Xu; D Teplow; T D Lee; J Abelson
Journal: Biochemistry Date: 1990-07-03 Impact factor: 3.162

6. Xylose (glucose) isomerase gene from the thermophile Thermus thermophilus: cloning, sequencing, and comparison with other thermostable xylose isomerases.

Authors: K Dekker; H Yamagata; K Sakaguchi; S Udaka
Journal: J Bacteriol Date: 1991-05 Impact factor: 3.490

7. The nucleotide sequence of the DNA ligase gene (CDC9) from Saccharomyces cerevisiae: a gene which is cell-cycle regulated and induced in response to DNA damage.

Authors: D G Barker; J H White; L H Johnston
Journal: Nucleic Acids Res Date: 1985-12-09 Impact factor: 16.971

8. An improved assay for DNA ligase reveals temperature-sensitive activity in cdc9 mutants of Saccharomyces cerevisiae.

Authors: D G Barker; A L Johnson; L H Johnston
Journal: Mol Gen Genet Date: 1985

9. Molecular characterisation of the DNA ligase gene, CDC17, from the fission yeast Schizosaccharomyces pombe.

Authors: D G Barker; J H White; L H Johnston
Journal: Eur J Biochem Date: 1987-02-02

10. Thermophilic HB8 DNA ligase: effects of polyethylene glycols and polyamines on blunt-end ligation of DNA.

Authors: M Takahashi; T Uchida
Journal: J Biochem Date: 1986-07 Impact factor: 3.387

15 in total

1. Transcriptional repressor of vasoactive intestinal peptide receptor mediates repression through interactions with TFIIB and TFIIEbeta.

Authors: L Pei
Journal: Biochem J Date: 2001-12-15 Impact factor: 3.857

9. Active site of the mRNA-capping enzyme guanylyltransferase from Saccharomyces cerevisiae: similarity to the nucleotidyl attachment motif of DNA and RNA ligases.

Authors: L D Fresco; S Buratowski
Journal: Proc Natl Acad Sci U S A Date: 1994-07-05 Impact factor: 11.205

10. Molecular characterisation of a DNA ligase gene of the extremely thermophilic archaeon Desulfurolobus ambivalens shows close phylogenetic relationship to eukaryotic ligases.

Authors: A Kletzin
Journal: Nucleic Acids Res Date: 1992-10-25 Impact factor: 16.971

Cloning, nucleotide sequence, and engineered expression of Thermus thermophilus DNA ligase, a homolog of Escherichia coli DNA ligase.

1. Transcriptional mapping and nucleotide sequence of a vaccinia virus gene encoding a polypeptide with extensive homology to DNA ligases.

2. Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli.

3. Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage.

4. Information content of binding sites on nucleotide sequences.

5. Domain structure in yeast tRNA ligase.

6. Xylose (glucose) isomerase gene from the thermophile Thermus thermophilus: cloning, sequencing, and comparison with other thermostable xylose isomerases.

7. The nucleotide sequence of the DNA ligase gene (CDC9) from Saccharomyces cerevisiae: a gene which is cell-cycle regulated and induced in response to DNA damage.

8. An improved assay for DNA ligase reveals temperature-sensitive activity in cdc9 mutants of Saccharomyces cerevisiae.

9. Molecular characterisation of the DNA ligase gene, CDC17, from the fission yeast Schizosaccharomyces pombe.

10. Thermophilic HB8 DNA ligase: effects of polyethylene glycols and polyamines on blunt-end ligation of DNA.

1. Transcriptional repressor of vasoactive intestinal peptide receptor mediates repression through interactions with TFIIB and TFIIEbeta.

2. Cloning and functional characterization of an NAD(+)-dependent DNA ligase from Staphylococcus aureus.

3. Identification of essential residues in Thermus thermophilus DNA ligase.

4. Control of 5',5'-dinucleoside triphosphate catabolism by APH1, a Saccharomyces cerevisiae analog of human FHIT.

5. A new protein motif found in DNA joining and DNA binding proteins.

6. Cloning of the large subunit of activator 1 (replication factor C) reveals homology with bacterial DNA ligases.

7. Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.

8. Properties of the lysyl-tRNA synthetase gene and product from the extreme thermophile Thermus thermophilus.

9. Active site of the mRNA-capping enzyme guanylyltransferase from Saccharomyces cerevisiae: similarity to the nucleotidyl attachment motif of DNA and RNA ligases.

10. Molecular characterisation of a DNA ligase gene of the extremely thermophilic archaeon Desulfurolobus ambivalens shows close phylogenetic relationship to eukaryotic ligases.