Literature DB >> 3900045

Isolation of a dihydrofolate reductase-deficient mutant of Escherichia coli.

S Singer, R Ferone, L Walton, L Elwell.   

Abstract

A strain of Escherichia coli was isolated in which dihydrofolate reductase was not detected by an enzyme assay or by competition for antibody. This strain requires methionine, glycine, a purine, and thymidine for growth in addition to the auxotrophic requirements of the parent strain. It was found to be useful as a recipient of plasmids harboring dihydrofolate reductase genes.

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Year:  1985        PMID： 3900045      PMCID： PMC214268          DOI： 10.1128/jb.164.1.470-472.1985

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  12 in total

1.  Two distinct types of trimethoprim-resistant dihydrofolate reductase specified by R-plasmids of different compatibility groups.

Authors:  K H Pattishall; J Acar; J J Burchall; F W Goldstein; R J Harvey
Journal:  J Biol Chem       Date:  1977-04-10       Impact factor: 5.157

2.  Use of Brij lysis as a general method to prepare polyribosomes from Escherichia coli.

Authors:  G N Godson; R L Sinsheimer
Journal:  Biochim Biophys Acta       Date:  1967-12-19

3.  Cloning of the Escherichia coli K-12 dihydrofolate reductase gene following mu-mediated transposition.

Authors:  J I Rood; A J Laird; J W Williams
Journal:  Gene       Date:  1980-02       Impact factor: 3.688

4.  Protein expression in Escherichia coli minicells containing recombinant plasmids specifying trimethoprim-resistant dihydrofolate reductases.

Authors:  M E Fling; L P Elwell
Journal:  J Bacteriol       Date:  1980-02       Impact factor: 3.490

5.  Dihydrofolate reductase: thymidylate synthase, a bifunctional polypeptide from Crithidia fasciculata.

Authors:  R Ferone; S Roland
Journal:  Proc Natl Acad Sci U S A       Date:  1980-10       Impact factor: 11.205

6.  Escherichia coli dihydrofolate reductase: isolation and characterization of two isozymes.

Authors:  D P Baccanari; D Averett; C Briggs; J Burchall
Journal:  Biochemistry       Date:  1977-08-09       Impact factor: 3.162

7.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

8.  Growth and initiation of protein synthesis in Escherichia coli in the presence of trimethoprim.

Authors:  R J Harvey
Journal:  J Bacteriol       Date:  1973-04       Impact factor: 3.490

9.  Regulation of dihydrofolate reductase synthesis in Escherichia coli.

Authors:  D R Smith; J M Calvo
Journal:  Mol Gen Genet       Date:  1979-08

10.  Biopterin cofactor biosynthesis: independent regulation of GTP cyclohydrolase in adrenal medulla and cortex.

Authors:  O H Viveros; C L Lee; M M Abou-Donia; J C Nixon; C A Nichol
Journal:  Science       Date:  1981-07-17       Impact factor: 47.728
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  10 in total

1.  Isolation and nucleotide sequence of the hmp gene that encodes a haemoglobin-like protein in Escherichia coli K-12.

Authors:  S G Vasudevan; W L Armarego; D C Shaw; P E Lilley; N E Dixon; R K Poole
Journal:  Mol Gen Genet       Date:  1991-04

2.  Dihydropteridine reductase from Escherichia coli.

Authors:  S G Vasudevan; D C Shaw; W L Armarego
Journal:  Biochem J       Date:  1988-10-15       Impact factor: 3.857

3.  Construction of a fol mutant strain of Escherichia coli for use in dihydrofolate reductase mutagenesis experiments.

Authors:  P M Ahrweiler; C Frieden
Journal:  J Bacteriol       Date:  1988-07       Impact factor: 3.490

4.  Systems-level response to point mutations in a core metabolic enzyme modulates genotype-phenotype relationship.

Authors:  Shimon Bershtein; Jeong-Mo Choi; Sanchari Bhattacharyya; Bogdan Budnik; Eugene Shakhnovich
Journal:  Cell Rep       Date:  2015-04-16       Impact factor: 9.423

5.  The former annotated human pseudogene dihydrofolate reductase-like 1 (DHFRL1) is expressed and functional.

Authors:  Gráinne McEntee; Stefano Minguzzi; Kirsty O'Brien; Nadia Ben Larbi; Christine Loscher; Ciarán O'Fágáin; Anne Parle-McDermott
Journal:  Proc Natl Acad Sci U S A       Date:  2011-08-26       Impact factor: 11.205

6.  In Vivo Titration of Folate Pathway Enzymes.

Authors:  Deepika Nambiar; Timkhite-Kulu Berhane; Robert Shew; Bryan Schwarz; Michael R Duff; Elizabeth E Howell
Journal:  Appl Environ Microbiol       Date:  2018-09-17       Impact factor: 4.792

7.  6-pyruvoyltetrahydropterin synthase paralogs replace the folate synthesis enzyme dihydroneopterin aldolase in diverse bacteria.

Authors:  Anne Pribat; Linda Jeanguenin; Aurora Lara-Núñez; Michael J Ziemak; John E Hyde; Valérie de Crécy-Lagard; Andrew D Hanson
Journal:  J Bacteriol       Date:  2009-04-24       Impact factor: 3.490

8.  Construction of a dihydrofolate reductase-deficient mutant of Escherichia coli by gene replacement.

Authors:  E E Howell; P G Foster; L M Foster
Journal:  J Bacteriol       Date:  1988-07       Impact factor: 3.490

9.  Isolation and expression of the Pneumocystis carinii dihydrofolate reductase gene.

Authors:  J C Edman; U Edman; M Cao; B Lundgren; J A Kovacs; D V Santi
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

10.  Development of antibacterial compounds that constrain evolutionary pathways to resistance.

Authors:  Yanmin Zhang; Sourav Chowdhury; João V Rodrigues; Eugene Shakhnovich
Journal:  Elife       Date:  2021-07-19       Impact factor: 8.140
  10 in total

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