Literature DB >> 3060113

Dihydropteridine reductase from Escherichia coli.

S G Vasudevan1, D C Shaw, W L Armarego.   

Abstract

A dihydropteridine reductase from Escherichia coli was purified to apparent homogeneity. It is a dimeric enzyme with identical subunits (Mr 27000) and a free N-terminal group. It can use NADH (Vmax./Km 3.36 s-1) and NADPH (Vmax./Km 1.07 s-1) when 6-methyldihydro-(6H)-pterin is the second substrate, as well as quinonoid dihydro-(6H)-biopterin (Vmax./Km 0.69 s-1), dihydro-(6H)-neopterin (Vmax./Km 0.58 s-1), dihydro-(6H)-monapterin 0.66 s-1), 6-methyldihydro-(6H)-pterin and cis-6,7-dimethyldihydro-(6H)-pterin (Vmax./Km 0.66 s-1) when NADH is the second substrate. The pure reductase has a yellow colour and contains bound FAD. The enzyme also has pterin-independent NADH and NADPH oxidoreductase activities when potassium ferricyanide is the electron acceptor.

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Year:  1988        PMID： 3060113      PMCID： PMC1135267     

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  23 in total

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Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

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Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

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Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Isolation and characterization of dihydropteridine reductase from Pseudomonas species.

Authors:  C D Williams; G Dickens; C H Letendre; G Guroff; C Haines; T Shiota
Journal:  J Bacteriol       Date:  1976-09       Impact factor: 3.490

5.  Tetrahydropteridine-dependent cleavage enzyme for O-alkyl lipids: substrate specificity.

Authors:  F Snyder; B Malone; C Piantadosi
Journal:  Biochim Biophys Acta       Date:  1973-08-23

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Authors:  H Rembold; K Buff
Journal:  Eur J Biochem       Date:  1972-08-04

7.  A naphthoquinone adsorbent for affinity chromatography of human dihydropteridine reductase.

Authors:  R G Cotton; I Jennings
Journal:  Eur J Biochem       Date:  1978-02-01

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Authors:  G Guroff; C A Rhoads
Journal:  J Biol Chem       Date:  1969-01-10       Impact factor: 5.157

9.  The purification of rat and sheep liver dihydropteridine reductases by affinity chromatography on methotrexate-sepharose.

Authors:  S Webber; T L Deits; W R Snyder; J M Whiteley
Journal:  Anal Biochem       Date:  1978-02       Impact factor: 3.365

10.  Purification of dihydropterin reductase using immobilized Cibacron Blue.

Authors:  M M Chauvin; K K Korri; A Tirpak; R C Simpson; K G Scrimgeour
Journal:  Can J Biochem       Date:  1979-02
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  9 in total

1.  Isolation and nucleotide sequence of the hmp gene that encodes a haemoglobin-like protein in Escherichia coli K-12.

Authors:  S G Vasudevan; W L Armarego; D C Shaw; P E Lilley; N E Dixon; R K Poole
Journal:  Mol Gen Genet       Date:  1991-04

2.  High-level expression of human dihydropteridine reductase (EC 1.6.99.7), without N-terminal amino acid protection, in Escherichia coli.

Authors:  W L Armarego; R G Cotton; H H Dahl; N E Dixon
Journal:  Biochem J       Date:  1989-07-01       Impact factor: 3.857

3.  PhhB, a Pseudomonas aeruginosa homolog of mammalian pterin 4a-carbinolamine dehydratase/DCoH, does not regulate expression of phenylalanine hydroxylase at the transcriptional level.

Authors:  J Song; T Xia; R A Jensen
Journal:  J Bacteriol       Date:  1999-05       Impact factor: 3.490

Review 4.  Biodegradation of aromatic compounds by Escherichia coli.

Authors:  E Díaz; A Ferrández; M A Prieto; J L García
Journal:  Microbiol Mol Biol Rev       Date:  2001-12       Impact factor: 11.056

Review 5.  Functions of the gene products of Escherichia coli.

Authors:  M Riley
Journal:  Microbiol Rev       Date:  1993-12

6.  FolX and FolM are essential for tetrahydromonapterin synthesis in Escherichia coli and Pseudomonas aeruginosa.

Authors:  Anne Pribat; Ian K Blaby; Aurora Lara-Núñez; Jesse F Gregory; Valérie de Crécy-Lagard; Andrew D Hanson
Journal:  J Bacteriol       Date:  2009-11-06       Impact factor: 3.490

7.  Dihydropteridine reductase as an alternative to dihydrofolate reductase for synthesis of tetrahydrofolate in Thermus thermophilus.

Authors:  Valérie Wilquet; Mark Van de Casteele; Daniel Gigot; Christianne Legrain; Nicolas Glansdorff
Journal:  J Bacteriol       Date:  2004-01       Impact factor: 3.490

8.  Comparative genomics of bacterial and plant folate synthesis and salvage: predictions and validations.

Authors:  Valérie de Crécy-Lagard; Basma El Yacoubi; Rocío Díaz de la Garza; Alexandre Noiriel; Andrew D Hanson
Journal:  BMC Genomics       Date:  2007-07-23       Impact factor: 3.969

9.  Enhanced synthesis of 5-hydroxy-l-tryptophan through tetrahydropterin regeneration.

Authors:  Ryotaro Hara; Kuniki Kino
Journal:  AMB Express       Date:  2013-12-09       Impact factor: 3.298
  9 in total

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