Literature DB >> 3899155

Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry.

J Cordell, T C Richardson, K A Pulford, A K Ghosh, K C Gatter, E Heyderman, D Y Mason.   

Abstract

Two monoclonal murine antibodies have been raised against a delipidated extract of human cream. These antibodies were detected by immunohistological screening of hybridoma culture supernatants on sections of human breast tissue. One of those antibodies (E29) was subsequently screened against a range of normal and neoplastic human tissues and shown to react with a wide variety of human epithelia and with mesothelial cells. Antibody E29 was unreactive with other cell types, with the exception of occasional plasma cells. Antibody E29 is suitable for use on paraffin embedded tissue and represents a valuable reagent for the identification of tumours of epithelial origin.

Entities:  

Mesh:

Substances:

Year:  1985        PMID: 3899155      PMCID: PMC1977193          DOI: 10.1038/bjc.1985.200

Source DB:  PubMed          Journal:  Br J Cancer        ISSN: 0007-0920            Impact factor:   7.640


  23 in total

1.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

2.  A new antigen on the epithelial membrane: its immunoperoxidase localisation in normal and neoplastic tissue.

Authors:  E Heyderman; K Steele; M G Ormerod
Journal:  J Clin Pathol       Date:  1979-01       Impact factor: 3.411

3.  Surface differentiation antigens of human mammary epithelial cells carried on the human milk fat globule.

Authors:  R L Ceriani; K Thompson; J A Peterson; S Abraham
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Monoclonal antibodies to the human mammary gland. I. Distribution of determinants in non-neoplastic mammary and extra mammary tissues.

Authors:  C S Foster; P A Edwards; E A Dinsdale; A M Neville
Journal:  Virchows Arch A Pathol Anat Histol       Date:  1982

6.  An immunocytochemical study of the distribution of epithelial membrane antigen in normal and disordered squamous epithelium.

Authors:  J P Sloane; M G Ormerod; R L Carter; B A Gusterson; C S Foster
Journal:  Diagn Histopathol       Date:  1982 Jan-Mar

7.  Distribution of epithelial membrane antigen in normal and neoplastic tissues and it value in diagnostic tumor pathology.

Authors:  J P Sloane; M G Ormerod
Journal:  Cancer       Date:  1981-04-01       Impact factor: 6.860

8.  Differentiation antigens expressed by epithelial cells in the lactating breast are also detectable in breast cancers.

Authors:  J Arklie; J Taylor-Papadimitrious; W Bodmer; M Egan; R Millis
Journal:  Int J Cancer       Date:  1981-07-15       Impact factor: 7.396

9.  Use of antisera to epithelial membrane antigen for the cytodiagnosis of malignancy in serous effusions.

Authors:  A To; D V Coleman; D P Dearnaley; M G Ormerod; K Steele; A M Neville
Journal:  J Clin Pathol       Date:  1981-12       Impact factor: 3.411

10.  The use of antisera to epithelial membrane antigen in detecting micrometastases in histological sections.

Authors:  J P Sloane; M G Ormerod; S F Imrie; R C Coombes
Journal:  Br J Cancer       Date:  1980-09       Impact factor: 7.640

View more
  35 in total

1.  Coexpression of epithelial membrane antigen (EMA), Ki-1, and interleukin-2 receptor by anaplastic large cell lymphomas. Diagnostic value in so-called malignant histiocytosis.

Authors:  G Delsol; T Al Saati; K C Gatter; J Gerdes; R Schwarting; P Caveriviere; F Rigal-Huguet; A Robert; H Stein; D Y Mason
Journal:  Am J Pathol       Date:  1988-01       Impact factor: 4.307

2.  Expression of epithelial membrane antigen by carcinoid tumours.

Authors:  A Gledhill; C Sparham; M F Dixon
Journal:  J Clin Pathol       Date:  1990-08       Impact factor: 3.411

3.  Epithelial membrane antigen and cytokeratin expression by meningiomas: an immunohistological study.

Authors:  J M Theaker; K C Gatter; M M Esiri; K A Fleming
Journal:  J Clin Pathol       Date:  1986-04       Impact factor: 3.411

4.  Effect of decalcification agents on immunoreactivity of cellular antigens.

Authors:  N A Athanasou; J Quinn; A Heryet; C G Woods; J O McGee
Journal:  J Clin Pathol       Date:  1987-08       Impact factor: 3.411

5.  Micrometastatic cancer cells in bone marrow: in vitro detection with anti-cytokeratin and in vivo labeling with anti-17-1A monoclonal antibodies.

Authors:  G Schlimok; I Funke; B Holzmann; G Göttlinger; G Schmidt; H Häuser; S Swierkot; H H Warnecke; B Schneider; H Koprowski; G Riethmüller
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

6.  Immunohistochemical observations of keratins, involucrin, and epithelial membrane antigen in urinary bladder carcinomas from patients infected with Schistosoma haematobium.

Authors:  S Fukushima; N Ito; M N el-Bolkainy; H N Tawfik; Y Tatemoto; M Mori
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1987

7.  Use of immunocytochemical staining to identify cells in peritoneal fluid and washings at laparoscopy and laparotomy.

Authors:  A K Ghosh; A I Spriggs; M Charnock
Journal:  J Clin Pathol       Date:  1986-12       Impact factor: 3.411

8.  Intraepithelial lymphocytes and macrophages in the normal breast.

Authors:  D J Ferguson
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1985

9.  MUC1 (EMA) is preferentially expressed by ALK positive anaplastic large cell lymphoma, in the normally glycosylated or only partly hypoglycosylated form.

Authors:  R L ten Berge; F G Snijdewint; S von Mensdorff-Pouilly; R J Poort-Keesom; J J Oudejans; J W Meijer; R Willemze; J Hilgers; C J Meijer
Journal:  J Clin Pathol       Date:  2001-12       Impact factor: 3.411

10.  KL-6 is another useful marker in assessing a micropapillary pattern in carcinomas of the breast and urinary bladder, but not the colon.

Authors:  Yuji Ohtsuki; Naoto Kuroda; Tatsuo Umeoka; Ryohei Watanabe; Kenji Ochi; Yuhei Okada; Gang-Hong Lee; Mutsuo Furihata
Journal:  Med Mol Morphol       Date:  2009-06-18       Impact factor: 2.309

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.