Literature DB >> 3888112

Cloning and expression in Escherichia coli of pectinase genes of Erwinia carotovora subsp. carotovora.

R T Zink, A K Chatterjee.   

Abstract

Genes coding for an endo-pectate lyase, an exo-pectate lyase, and an endopolygalacturonase of Erwinia carotovora subsp. carotovora Ecc71 were cloned in Escherichia coli HB101, using the cosmid pHC79. The products of the cloned pectinase genes paralleled their counterparts in strain Ecc71 in isoelectric mobility, mode of substrate degradation, and ability to macerate potato tuber tissue.

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Year:  1985        PMID: 3888112      PMCID: PMC373578          DOI: 10.1128/aem.49.3.714-717.1985

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  13 in total

1.  Escherichia coli plasmids packageable in vitro in lambda bacteriophage particles.

Authors:  J Collins
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

Review 2.  Pectinases and pectic polysaccharides.

Authors:  W M Fogarty; O P Ward
Journal:  Prog Ind Microbiol       Date:  1974

Review 3.  Genetics of Erwinia species.

Authors:  A K Chatterjee; M P Starr
Journal:  Annu Rev Microbiol       Date:  1980       Impact factor: 15.500

4.  Utilization of plasmid pULB113 (RP4::mini-Mu) to construct a linkage map of Erwinia carotovora subsp. chrysanthemi.

Authors:  E Schoonejans; A Toussaint
Journal:  J Bacteriol       Date:  1983-06       Impact factor: 3.490

5.  Donor strains of the soft-rot bacterium Erwinia chrysanthemi and conjugational transfer of the pectolytic capacity.

Authors:  A K Chatterjee; M P Starr
Journal:  J Bacteriol       Date:  1977-12       Impact factor: 3.490

6.  Hydrolytic and trans-eliminative degradation of pectic substances by extracellular enzymes of Fusarium solani f. phaseoli.

Authors:  D F Bateman
Journal:  Phytopathology       Date:  1966-02       Impact factor: 4.025

7.  Acceptance by Erwinia spp. of R plasmid R68.45 and its ability to mobilize the chromosome of Erwinia chrysanthemi.

Authors:  A K Chatterjee
Journal:  J Bacteriol       Date:  1980-04       Impact factor: 3.490

8.  Molecular cloning of pectate lyase genes from Erwinia chrysanthemi and their expression in Escherichia coli.

Authors:  N T Keen; D Dahlbeck; B Staskawicz; W Belser
Journal:  J Bacteriol       Date:  1984-09       Impact factor: 3.490

9.  An exo-poly-alpha-D-galacturonosidase implicated in the regulation of extracellular pectate lyase production in Erwinia chrysanthemi.

Authors:  A Collmer; C H Whalen; S V Beer; D F Bateman
Journal:  J Bacteriol       Date:  1982-02       Impact factor: 3.490

10.  Utilization of a thermosensitive episome bearing transposon TN10 to isolate Hfr donor strains of Erwinia carotovora subsp. chrysanthemi.

Authors:  A Kotoujansky; M Lemattre; P Boistard
Journal:  J Bacteriol       Date:  1982-04       Impact factor: 3.490

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  14 in total

1.  A Rapid Procedure for Purifying Pectate Lyase from Erwinia carotovora Based on Substrate Affinity.

Authors:  L Ward; S H de Boer
Journal:  Appl Environ Microbiol       Date:  1987-05       Impact factor: 4.792

2.  Multiplication and Virulence in Plant Tissues of Escherichia coli Clones Producing Pectate Lyase Isozymes PLb and PLe at High Levels and of an Erwinia chrysanthemi Mutant Deficient in PLe.

Authors:  J H Payne; C Schoedel; N T Keen; A Collmer
Journal:  Appl Environ Microbiol       Date:  1987-10       Impact factor: 4.792

3.  Single-site chromosomal Tn5 insertions affect the export of pectolytic and cellulolytic enzymes in Erwinia chrysanthemi EC16.

Authors:  K K Thurn; A K Chatterjee
Journal:  Appl Environ Microbiol       Date:  1985-10       Impact factor: 4.792

4.  Evidence of homology between the pectate lyase-encoding pelB and pelC genes in Erwinia chrysanthemi.

Authors:  C Schoedel; A Collmer
Journal:  J Bacteriol       Date:  1986-07       Impact factor: 3.490

5.  Requirement for two or more Erwinia carotovora subsp. carotovora pectolytic gene products for maceration of potato tuber tissue by Escherichia coli.

Authors:  D P Roberts; P M Berman; C Allen; V K Stromberg; G H Lacy; M S Mount
Journal:  J Bacteriol       Date:  1986-07       Impact factor: 3.490

6.  Endopolygalacturonase is not required for pathogenicity of Cochliobolus carbonum on maize.

Authors:  J S Scott-Craig; D G Panaccione; F Cervone; J D Walton
Journal:  Plant Cell       Date:  1990-12       Impact factor: 11.277

7.  recA is required in the induction of pectin lyase and carotovoricin in Erwinia carotovora subsp. carotovora.

Authors:  R T Zink; J K Engwall; J L McEvoy; A K Chatterjee
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490

8.  Structure of two pectate lyase genes from Erwinia chrysanthemi EC16 and their high-level expression in Escherichia coli.

Authors:  N T Keen; S Tamaki
Journal:  J Bacteriol       Date:  1986-11       Impact factor: 3.490

9.  Co-expression of an Erwinia chrysanthemi pectate lyase-encoding gene (pelE) and an E. carotovora polygalacturonase-encoding gene (peh1) in Saccharomyces cerevisiae.

Authors:  E Laing; I S Pretorius
Journal:  Appl Microbiol Biotechnol       Date:  1993-05       Impact factor: 4.813

10.  Expression of pehA-bla gene fusions in Erwinia carotovora subsp. carotovora and isolation of regulatory mutants affecting polygalacturonase production.

Authors:  H T Saarilahti; M Pirhonen; M B Karlsson; D Flego; E T Palva
Journal:  Mol Gen Genet       Date:  1992-07
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