Literature DB >> 16347348

A Rapid Procedure for Purifying Pectate Lyase from Erwinia carotovora Based on Substrate Affinity.

L Ward1, S H de Boer.   

Abstract

To purify pectate lyase produced by Erwinia carotovora subsp. carotovora, we used the supernatant from 48-h-old cultures grown in broth containing sodium polypectate and yeast extract. The supernatant was combined with the enzyme substrates sodium polypectate and polygalacturonic acid, which were then precipitated with CaCl(2). After the precipitate was washed, pectate lyase was eluted with 1.0 M NaCl.

Entities:  

Year:  1987        PMID： 16347348      PMCID： PMC203833          DOI： 10.1128/aem.53.5.1189-1191.1987

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

1.  Activity stain for rapid characterization of pectic enzymes in isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gels.

Authors:  J L Ried; A Collmer
Journal:  Appl Environ Microbiol       Date:  1985-09       Impact factor: 4.792

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Cloning and expression in Escherichia coli of pectinase genes of Erwinia carotovora subsp. carotovora.

Authors:  R T Zink; A K Chatterjee
Journal:  Appl Environ Microbiol       Date:  1985-03       Impact factor: 4.792

4.  Cloning of the pectate lyase genes from Erwinia carotovora and their expression in Escherichia coli.

Authors:  S P Lei; H C Lin; L Heffernan; G Wilcox
Journal:  Gene       Date:  1985       Impact factor: 3.688

5.  Enzymatic degradation of polygalacturonic acid by Yersinia and Klebsiella species in relation to clinical laboratory procedures.

Authors:  M P Starr; A K Chatterjee; P B Starr; G E Buchanan
Journal:  J Clin Microbiol       Date:  1977-10       Impact factor: 5.948

6.  An exo-poly-alpha-D-galacturonosidase implicated in the regulation of extracellular pectate lyase production in Erwinia chrysanthemi.

Authors:  A Collmer; C H Whalen; S V Beer; D F Bateman
Journal:  J Bacteriol       Date:  1982-02       Impact factor: 3.490

7.  Evidence that polygalacturonase is a virulence determinant in Erwinia carotovora.

Authors:  S P Lei; H C Lin; L Heffernan; G Wilcox
Journal:  J Bacteriol       Date:  1985-11       Impact factor: 3.490

8.  Comparison of pectic enzymes produced by Erwinia chrysanthemi, Erwinia carotovora subsp. carotovora, and Erwinia carotovora subsp. atroseptica.

Authors:  J L Ried; A Collmer
Journal:  Appl Environ Microbiol       Date:  1986-08       Impact factor: 4.792
  8 in total
  2 in total

1.  Role of lysine, tryptophan and calcium in the beta-elimination activity of a low-molecular-mass pectate lyase from Fusarium moniliformae.

Authors:  M N Rao; A A Kembhavi; A Pant
Journal:  Biochem J       Date:  1996-10-01       Impact factor: 3.857

2.  Facile isolation of endo-pectate lyase from Erwinia carotovora based on electrostatic interaction.

Authors:  S Fukuoka; H Kamishima; K Sode; I Karube
Journal:  Appl Biochem Biotechnol       Date:  1990-12       Impact factor: 2.926
  2 in total

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