Literature DB >> 3882998

Monoclonal antibodies specific for African swine fever virus proteins.

A Sanz, B García-Barreno, M L Nogal, E Viñuela, L Enjuanes.   

Abstract

We have obtained 60 stable hybridomas which produced immunoglobulins that recognized 12 proteins from African swine fever virus particles and African swine fever virus-infected cells. Most of the monoclonal antibodies were specific for the three major structural proteins p150, p72, and p12. The specificity of some monoclonal antibodies for the structural proteins p150 and p37 and the nonstructural proteins p220 and p60 indicated that proteins p150 and p220 are antigenically related to proteins p37 and p60. The association of some viral antigens to specific subcellular components was determined by immunofluorescence and analysis of the binding of monoclonal antibodies to infected cells. A host protein (p24) seemed to be associated with the virus particles.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 3882998      PMCID： PMC254778     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  17 in total

1.  A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.

Authors:  J F Kearney; A Radbruch; B Liesegang; K Rajewsky
Journal:  J Immunol       Date:  1979-10       Impact factor: 5.422

2.  Continuous cultures of fused cells secreting antibody of predefined specificity.

Authors:  G Köhler; C Milstein
Journal:  Nature       Date:  1975-08-07       Impact factor: 49.962

3.  Hemadsorption and cytopathic effect produced by African Swine Fever virus in swine bone marrow and buffy coat cultures.

Authors:  W A MALMQUIST; D HAY
Journal:  Am J Vet Res       Date:  1960-01       Impact factor: 1.156

4.  The isolation of hybrid cell lines producing monoclonal antibodies against the p15(E) protein of ecotropic murine leukemia viruses.

Authors:  R C Nowinski; M E Lostrom; M R Tam; M R Stone; W N Burnette
Journal:  Virology       Date:  1979-02       Impact factor: 3.616

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  High frequencies of antigen-specific hybridomas: dependence on immunization parameters and prediction by spleen cell analysis.

Authors:  C Stähli; T Staehelin; V Miggiano; J Schmidt; P Häring
Journal:  J Immunol Methods       Date:  1980       Impact factor: 2.303

7.  Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.

Authors:  J P Chamberlain
Journal:  Anal Biochem       Date:  1979-09-15       Impact factor: 3.365

8.  Titration of African swine fever (ASF) virus.

Authors:  L Enjuanes; A L Carrascosa; M A Moreno; E Viñuela
Journal:  J Gen Virol       Date:  1976-09       Impact factor: 3.891

9.  Specific incorporation of host cell surface proteins into budding vesicular stomatitis virus particles.

Authors:  H F Lodish; M Porter
Journal:  Cell       Date:  1980-01       Impact factor: 41.582

Review 10.  African swine fever: a reassessment.

Authors:  W R Hess
Journal:  Adv Vet Sci Comp Med       Date:  1981
View more
  53 in total

1.  Proteolytic processing in African swine fever virus: evidence for a new structural polyprotein, pp62.

Authors:  C Simón-Mateo; G Andrés; F Almazán; E Viñuela
Journal:  J Virol       Date:  1997-08       Impact factor: 5.103

2.  Neutralization of human respiratory syncytial virus infectivity by antibodies and low-molecular-weight compounds targeted against the fusion glycoprotein.

Authors:  Margarita Magro; David Andreu; Paulino Gómez-Puertas; José A Melero; Concepción Palomo
Journal:  J Virol       Date:  2010-06-09       Impact factor: 5.103

3.  African swine fever virus attachment protein.

Authors:  A L Carrascosa; I Sastre; E Viñuela
Journal:  J Virol       Date:  1991-05       Impact factor: 5.103

4.  African swine fever virus protein p17 is essential for the progression of viral membrane precursors toward icosahedral intermediates.

Authors:  Cristina Suárez; Javier Gutiérrez-Berzal; Germán Andrés; María L Salas; Javier M Rodríguez
Journal:  J Virol       Date:  2010-05-26       Impact factor: 5.103

5.  Expression in vivo and in vitro of the major structural protein (VP73) of African swine fever virus.

Authors:  C Cistué; E Tabarés
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

6.  Amino acid sequence and structural properties of protein p12, an African swine fever virus attachment protein.

Authors:  A Alcamí; A Angulo; C López-Otín; M Muñoz; J M Freije; A L Carrascosa; E Viñuela
Journal:  J Virol       Date:  1992-06       Impact factor: 5.103

7.  African swine fever virus structural protein pE120R is essential for virus transport from assembly sites to plasma membrane but not for infectivity.

Authors:  G Andrés; R García-Escudero; E Viñuela; M L Salas; J M Rodríguez
Journal:  J Virol       Date:  2001-08       Impact factor: 5.103

8.  African swine fever virus pB119L protein is a flavin adenine dinucleotide-linked sulfhydryl oxidase.

Authors:  Irene Rodríguez; Modesto Redrejo-Rodríguez; Javier M Rodríguez; Alí Alejo; José Salas; María L Salas
Journal:  J Virol       Date:  2006-04       Impact factor: 5.103

9.  Inducible gene expression from African swine fever virus recombinants: analysis of the major capsid protein p72.

Authors:  R García-Escudero; G Andrés; F Almazán; E Viñuela
Journal:  J Virol       Date:  1998-04       Impact factor: 5.103

Review 10.  A guide to viral inclusions, membrane rearrangements, factories, and viroplasm produced during virus replication.

Authors:  Christopher Netherton; Katy Moffat; Elizabeth Brooks; Thomas Wileman
Journal:  Adv Virus Res       Date:  2007       Impact factor: 9.937
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.