Literature DB >> 823294

Titration of African swine fever (ASF) virus.

L Enjuanes, A L Carrascosa, M A Moreno, E Viñuela.   

Abstract

A haemadsorption microtest for African swine fever (ASF) virus is described. This assay is as sensitive and its response is faster than the conventional assay which uses buffy coat cultures in Leighton tubes. The method can also process a larger number of samples by using smaller amounts of swine blood and laboratory space. A plaque assay for ASF virus adapted to grow in VERO cells gives a titre similar to that obtained using the haemadsorption microtest. In both the micromethod and the plaque assay infection may be produced by a single infective particle.

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Year:  1976        PMID： 823294     DOI： 10.1099/0022-1317-32-3-471

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  106 in total

1.  Structure of African swine fever virus late promoters: requirement of a TATA sequence at the initiation region.

Authors:  R García-Escudero; E Viñuela
Journal:  J Virol       Date:  2000-09       Impact factor: 5.103

2.  Proteolytic processing in African swine fever virus: evidence for a new structural polyprotein, pp62.

Authors:  C Simón-Mateo; G Andrés; F Almazán; E Viñuela
Journal:  J Virol       Date:  1997-08       Impact factor: 5.103

3.  Transcriptional analysis of multigene family 110 of African swine fever virus.

Authors:  F Almazán; J M Rodríguez; G Andrés; R Pérez; E Viñuela; J F Rodriguez
Journal:  J Virol       Date:  1992-11       Impact factor: 5.103

4.  African swine fever virus attachment protein.

Authors:  A L Carrascosa; I Sastre; E Viñuela
Journal:  J Virol       Date:  1991-05       Impact factor: 5.103

5.  African swine fever virus protein p17 is essential for the progression of viral membrane precursors toward icosahedral intermediates.

Authors:  Cristina Suárez; Javier Gutiérrez-Berzal; Germán Andrés; María L Salas; Javier M Rodríguez
Journal:  J Virol       Date:  2010-05-26       Impact factor: 5.103

6.  Comparison of the sequence of the gene encoding African swine fever virus attachment protein p12 from field virus isolates and viruses passaged in tissue culture.

Authors:  A Angulo; E Viñuela; A Alcamí
Journal:  J Virol       Date:  1992-06       Impact factor: 5.103

7.  Nuclear export of African swine fever virus p37 protein occurs through two distinct pathways and is mediated by three independent signals.

Authors:  Ana Eulálio; Isabel Nunes-Correia; Ana Luísa Carvalho; Carlos Faro; Vitaly Citovsky; José Salas; Maria L Salas; Sérgio Simões; Maria C Pedroso de Lima
Journal:  J Virol       Date:  2006-02       Impact factor: 5.103

8.  African swine fever virus structural protein pE120R is essential for virus transport from assembly sites to plasma membrane but not for infectivity.

Authors:  G Andrés; R García-Escudero; E Viñuela; M L Salas; J M Rodríguez
Journal:  J Virol       Date:  2001-08       Impact factor: 5.103

9.  Porcine leukocyte cellular subsets sensitive to African swine fever virus in vitro.

Authors:  I Casal; L Enjuanes; E Viñuela
Journal:  J Virol       Date:  1984-10       Impact factor: 5.103

10.  Hairpin loop structure of African swine fever virus DNA.

Authors:  A González; A Talavera; J M Almendral; E Viñuela
Journal:  Nucleic Acids Res       Date:  1986-09-11       Impact factor: 16.971
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