Literature DB >> 3876767

Improved resolution of PI (alpha 1-antitrypsin) phenotypes by a large-scale immobilized pH gradient.

A Görg, W Postel, J Weser, W Patutschnick, H Cleve.   

Abstract

PI variants and PI M subtypes were examined by isoelectric focusing in a preformed immobilized pH gradient. Conditions were found that permit reliable classification of PI variants and of most PI M subtypes: the pH gradient ranges from 4.3 to 4.8 on a polyacrylamide gel with a length of 20 cm and a separation distance of approximately 16-17 cm (delta pH = 0.025 U/cm). The PI phenotypes observed are presented.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 3876767      PMCID： PMC1684691     

Source DB:  PubMed          Journal:  Am J Hum Genet        ISSN： 0002-9297            Impact factor:   11.025


  15 in total

1.  Classification of alpha1-antitrypsin (Pi) phenotypes by isoelectrofocusing. Distinction of six subtypes of the PiM phenotype.

Authors:  T Genz; J P Martin; H Cleve
Journal:  Hum Genet       Date:  1977-10-14       Impact factor: 4.132

2.  Gel gradient electrophoresis, isoelectric focusing and two-dimensional techniques in horizontal, ultrathin polyacrylamide layers.

Authors:  A Görg; W Postel; R Westermeier; E Gianazza; P G Righetti
Journal:  J Biochem Biophys Methods       Date:  1980-11

3.  Report of Nomenclature Meeting for alpha 1-antitrypsin, INSERM, Rouen/Bois-Guillaume-1978.

Authors:  D W Cox; A M Johnson; M K Fagerhol
Journal:  Hum Genet       Date:  1980       Impact factor: 4.132

4.  Reliable classification of six Pi M subtypes by separator isoelectric focusing.

Authors:  R R Frants; A W Eriksson
Journal:  Hum Hered       Date:  1978       Impact factor: 0.444

5.  Population genetics of alpha1-antitrypsin in the Netherlands. Description of a new electrophoretic variant.

Authors:  E C Klasen; C Franken; W S Volkers; L F Bernini
Journal:  Hum Genet       Date:  1977-07-26       Impact factor: 4.132

6.  [A new PiN mutation at the Pi locus in human populations].

Authors:  J Constans; M Viau
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1975-11-03

7.  Genetic variants of human B component (BF system) and alpha-1-antitrypsin (PI system) in a population from Sardinia.

Authors:  C Davrinche; R Charlionet; C Rivat; R Sesboue; M Bayorek; M Arnone
Journal:  Hum Hered       Date:  1984       Impact factor: 0.444

8.  A new method for determination of alpha-1-antitrypsin phenotypes using isoelectric focusing on polyacrylamide gel slabs.

Authors:  R C Allen; R A Harley; R C Talamo
Journal:  Am J Clin Pathol       Date:  1974-12       Impact factor: 2.493

9.  Alpha 1 antitrypsin M1: a new common genetically determined variant.

Authors:  F Kueppers
Journal:  Am J Hum Genet       Date:  1976-07       Impact factor: 11.025

10.  alpha1-antitrypsin: common subtypes of Pi M.

Authors:  R R Frants; A W Eriksson
Journal:  Hum Hered       Date:  1976       Impact factor: 0.444
View more
  2 in total

1.  Characterization of the gene and protein of the common alpha 1-antitrypsin normal M2 allele.

Authors:  T Nukiwa; M L Brantly; F Ogushi; G A Fells; R G Crystal
Journal:  Am J Hum Genet       Date:  1988-09       Impact factor: 11.025

2.  Alpha 1-antitrypsin Null(isola di procida): an alpha 1-antitrypsin deficiency allele caused by deletion of all alpha 1-antitrypsin coding exons.

Authors:  H Takahashi; R G Crystal
Journal:  Am J Hum Genet       Date:  1990-09       Impact factor: 11.025
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.