| Literature DB >> 3876073 |
R C Cooksey, N C Clark, C Thornsberry.
Abstract
A restriction fragment of plasmid pBR322 bearing the TEM-1 beta-lactamase structural gene was electroeluted from agarose gels after digestion with EcoRI and HinfI. The 1-kilobase fragment was 32P-labeled and used to examine genetic relationships with nucleic acids encoding seven other beta-lactamase classes. The probe hybridized only with TEM-2 and OXA-2 class plasmids.Entities:
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Year: 1985 PMID: 3876073 PMCID: PMC176331 DOI: 10.1128/AAC.28.1.154
Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN: 0066-4804 Impact factor: 5.191