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Literature DB >> 3259120

Detection of plasmid-mediated beta-lactamases with DNA probes.

Abstract

beta-Lactamase identification by colony hybridization with 32P-labeled DNA probes for TEM-1, SHV-1, OXA-1, OXA-2, PSE-1, PSE-2, and PSE-4 was compared with isoelectric focusing in 122 clinical isolates making a variety of enzyme types. All strains producing a probe-type enzyme gave a positive hybridization reaction. Cross-hybridization was observed between TEM-1 and TEM-2 or TLE-1, between SHV-1 and SHV-2, between OXA-1 and OXA-4, between OXA-2 and OXA-3 (weak), between PSE-2 and OXA-6 or OXA-5 (weak), and among PSE-1, PSE-4, and CARB-3. With allowance for such cross-hybridization, only six strains gave false-positive reactions, and the procedure was 99% specific.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1988 PMID： 3259120 PMCID： PMC172130 DOI： 10.1128/AAC.32.2.175

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

30 in total

1. The distribution of beta-lactamase genes on plasmids found in Pseudomonas.

Authors: G A Jacoby; M Matthew
Journal: Plasmid Date: 1979-01 Impact factor: 3.466

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

Review 3. Plasmid-mediated beta-lactamases of Gram-negative bacteria: properties and distribution.

Authors: M Matthew
Journal: J Antimicrob Chemother Date: 1979-07 Impact factor: 5.790

4. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.

Authors: J G Sutcliffe
Journal: Proc Natl Acad Sci U S A Date: 1978-08 Impact factor: 11.205

5. Antibiotic susceptibility testing by a standardized single disk method.

Authors: A W Bauer; W M Kirby; J C Sherris; M Turck
Journal: Am J Clin Pathol Date: 1966-04 Impact factor: 2.493

6. Colony hybridization.

Authors: M Grunstein; J Wallis
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

7. Sequence of PSE-2 beta-lactamase.

Authors: P Huovinen; S Huovinen; G A Jacoby
Journal: Antimicrob Agents Chemother Date: 1988-01 Impact factor: 5.191

8. The use of analytical isoelectric focusing for detection and identification of beta-lactamases.

Authors: A Mathew; A M Harris; M J Marshall; G W Ross
Journal: J Gen Microbiol Date: 1975-05

Review 9. The beta-lactamases of gram-negative bacteria and their role in resistance to beta-lactam antibiotics.

Authors: R B Sykes; M Matthew
Journal: J Antimicrob Chemother Date: 1976-06 Impact factor: 5.790

10. Molecular epidemiology of antibiotic resistance in salmonella from animals and human beings in the United States.

Authors: T F O'Brien; J D Hopkins; E S Gilleece; A A Medeiros; R L Kent; B O Blackburn; M B Holmes; J P Reardon; J M Vergeront; W L Schell; E Christenson; M L Bissett; E V Morse
Journal: N Engl J Med Date: 1982-07-01 Impact factor: 91.245

22 in total

Review 1. Molecular detection of antimicrobial resistance.

Authors: A C Fluit; M R Visser; F J Schmitz
Journal: Clin Microbiol Rev Date: 2001-10 Impact factor: 26.132

Review 2. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat.

Authors: P A Bradford
Journal: Clin Microbiol Rev Date: 2001-10 Impact factor: 26.132

3. Occurrence of aminoglycoside phosphotransferase subclass I and II structural genes among Enterobacteriaceae spp. isolated from meat samples.

Authors: A H Jayaratne; D L Collins-Thompson; J T Trevors
Journal: Appl Microbiol Biotechnol Date: 1990-08 Impact factor: 4.813

4. Development of a DNA probe from the deoxyribonucleotide sequence of a 3-N-aminoglycoside acetyltransferase [AAC(3)-I] resistance gene.

Authors: F C Tenover; K L Phillips; T Gilbert; P Lockhart; P J O'Hara; J J Plorde
Journal: Antimicrob Agents Chemother Date: 1989-04 Impact factor: 5.191

5. Problems in interpretation of piperacillin susceptibility of TEM-1 producing Escherichia coli in the disk diffusion test.

Authors: T Arstila; S Huovinen; P Huovinen
Journal: Eur J Clin Microbiol Infect Dis Date: 1991-12 Impact factor: 3.267

6. Sequence of the PSE-1 beta-lactamase gene.

Authors: P Huovinen; G A Jacoby
Journal: Antimicrob Agents Chemother Date: 1991-11 Impact factor: 5.191

7. Patterns and mechanisms of beta-lactam resistance among isolates of Escherichia coli from hospitals in the United States.

Authors: R Cooksey; J Swenson; N Clark; E Gay; C Thornsberry
Journal: Antimicrob Agents Chemother Date: 1990-05 Impact factor: 5.191