Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Molecular cloning and partial characterization of delta-crystallin cDNA sequences in a bacterial plasmid.

Literature DB >> 386337

Molecular cloning and partial characterization of delta-crystallin cDNA sequences in a bacterial plasmid.

Abstract

Double-stranded cDNA synthesized from delta-crystallin mRNA isolated from lens fiber cells of 15-day-old embryonic chicken was cloned in Escherichia coli chi 1776 in the Pst I site of the plasmid pBR322 by using the oligo(dC) . oligo(dG) joining procedure. Twelve Amps Tetr transformants contained sequences complementary to purified delta-crystallin [32P]cDNA. One of the recombinant clones (p delta Cr-2) had an insert of 1241 +/- 240 base pairs, as judged by R-looping analysis with purified delta-crystallin mRNA. The inserted cDNA represents at least 69% of the delta-crystallin coding sequences. p delta Cr-2 was further characterized by restriction analysis, protection of delta-crystallin [3H]cDNA from digestion by S1 nuclease, and hybrid-mediated arrest of delta-crystallin mRNA translation in vitro. p delta Cr-2 provides an invaluable probe for additional analysis of the primary structure, gene organization, and regulated synthesis of delta-crystallin, the principal protein synthesized during lens differentiation in the chicken embryo.

Entities: Chemical Species

Mesh：

Substances：

Year: 1979 PMID： 386337 PMCID： PMC383812 DOI： 10.1073/pnas.76.7.3299

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

34 in total

Molecular cloning and partial characterization of delta-crystallin cDNA sequences in a bacterial plasmid.

1. LENS DEVELOPMENT. II. THE DIFFERENTIATION OF EMBRYONIC CHICK LENS EPITHELIAL CELLS IN VITRO AND IN VIVO.

2. Electrophoretic and immunoelectrophoretic studies on the soluble proteins in the developing lens of birds.

3. Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.

4. Intervening sequence of DNA identified in the structural portion of a mouse beta-globin gene.

5. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.

6. A novel arrangement of the 18S and 28S sequences in a repeating unit of Drosophila melanogaster rDNA.

7. Chicken lysozyme gene contains several intervening sequences.

8. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

9. Recombinant plasmids containing Xenopus laevis globin structural genes derived from complementary DNA.

10. Protein expression in E. coli minicells by recombinant plasmids.

1. Conservation of delta-crystallin gene structure between ducks and chickens.

2. Nucleotide sequence of a chicken delta-crystallin gene.

3. Delta-crystallin genes become hypomethylated in postmitotic lens cells during chicken development.

4. Comparison of two delta-crystallin genes in the chicken.

Review 5. Delta crystallins and their nucleic acids.

6. Cytoplasmic RNA sequences complementary to cloned chick delta-crystallin cDNA show size heterogeneity.

7. Multiple gamma-crystallins of the mouse lens: fractionation of mRNAs by cDNA cloning.

8. Sequence of a complete chicken delta-crystallin cDNA.

9. Chicken lens delta-crystallin gene expression and methylation in several non-lens tissues.

10. Organization of delta-crystallin genes in the chicken.