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Literature DB >> 3858829

DNA sequence and characterization of human class II major histocompatibility complex beta chains from the DR1 haplotype.

J I Bell, P Estess, T St John, R Saiki, D L Watling, H A Erlich, H O McDevitt.

Abstract

Two HLA class II beta-chain clones from a cell line homozygous for the DR1 haplotype have been characterized and sequenced. They represent a DR beta chain (2918.4) and a DQ beta chain (2918.8). Clone 2918.4 has been used to select mRNA from a lymphoblastoid cell line, and this was injected into Xenopus oocytes with mRNA selected with a DR alpha chain. The translation products were immunoprecipitated with a beta-chain-specific monoclonal antibody and electrophoresed on two-dimensional gels. This revealed positive signals in the positions predicted for beta and alpha chains. Sequence comparisons of 2918.4 with previously published DR beta-chain sequences confirm the presence of two regions of variability in the membrane distal domain. Analysis of the sequence of 2918.8 identified it as a DQ beta chain identical to one previously published from a DR3,w6 cell line. We speculate, therefore, that the DQ beta sequence represents the DQ1 specificity shared by the DR1 and DRw6 haplotypes.

Entities: Gene Species

Mesh：

Substances：

Year: 1985 PMID： 3858829 PMCID： PMC397784 DOI： 10.1073/pnas.82.10.3405

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

15 in total

1. Use of synthetic oligonucleotide probes complementary to genes for human HLA-DR alpha and beta as extension primers for the isolation of 5'-specific genomic clones.

Authors: H K Das; P A Biro; S N Cohen; H A Erlich; A von Gabain; S K Lawrance; P G Lemaux; H O McDevitt; B M Peterlin; M F Schulz; A K Sood; S M Weissman
Journal: Proc Natl Acad Sci U S A Date: 1983-03 Impact factor: 11.205

2. Mapping of the genes encoding the HLA-DR alpha chain and the HLA-related antigens to a chromosome 6 deletion by using genomic blotting.

Authors: H A Erlich; D Stetler; R Saiki; P Gladstone; D Pious
Journal: Proc Natl Acad Sci U S A Date: 1983-04 Impact factor: 11.205

3. N-terminal amino acid sequences of the alpha and beta chains of HLA-DR1 and HLA-DR2 antigens.

Authors: L E Walker; R Hewick; M W Hunkapiller; L E Hood; W J Dreyer; R A Reisfeld
Journal: Biochemistry Date: 1983-01-04 Impact factor: 3.162

4. Isolation and identification of a cDNA clone corresponding to an HLA-DR antigen beta chain.

Authors: K Wiman; D Larhammar; L Claesson; K Gustafsson; L Schenning; P Bill; J Böhme; M Denaro; B Dobberstein; U Hammerling; S Kvist; B Servenius; J Sundelin; P A Peterson; L Rask
Journal: Proc Natl Acad Sci U S A Date: 1982-03 Impact factor: 11.205

5. Complete amino acid sequence of an HLA-DR antigen-like beta chain as predicted from the nucleotide sequence: similarities with immunoglobulins and HLA-A, -B, and -C antigens.

Authors: D Larhammar; L Schenning; K Gustafsson; K Wiman; L Claesson; L Rask; P A Peterson
Journal: Proc Natl Acad Sci U S A Date: 1982-06 Impact factor: 11.205

6. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

7. Isolation of a cDNA clone for the human HLA-DR antigen alpha chain by using a synthetic oligonucleotide as a hybridization probe.

Authors: D Stetler; H Das; J H Nunberg; R Saiki; R Sheng-Dong; K B Mullis; S M Weissman; H A Erlich
Journal: Proc Natl Acad Sci U S A Date: 1982-10 Impact factor: 11.205

8. [Primary structure of class II human histocompatibility antigens. 1st communication. Amino acid sequence of the N-terminal 198 residues of the beta chain of a HLA-Dw2,2;DR2,2-alloantigen (author's transl)].

Authors: H Kratzin; C Y Yang; H Götz; E Pauly; S Kölbel; G Egert; F P Thinnes; P Wernet; P Altevogt; N Hilschmann
Journal: Hoppe Seylers Z Physiol Chem Date: 1981-12

9. Cloning and sequence analysis of the human major histocompatibility complex gene DC-3 beta.

Authors: J M Boss; J L Strominger
Journal: Proc Natl Acad Sci U S A Date: 1984-08 Impact factor: 11.205

10. Complete sequence of an HLA-dR beta chain deduced from a cDNA clone and identification of multiple non-allelic DR beta chain genes.

Authors: E O Long; C T Wake; J Gorski; B Mach
Journal: EMBO J Date: 1983 Impact factor: 11.598

34 in total

1. MHC evolution in three salmonid species: a comparison between class II alpha and beta genes.

Authors: Daniela Gómez; Pablo Conejeros; Sergio H Marshall; Sofia Consuegra
Journal: Immunogenetics Date: 2010-06-03 Impact factor: 2.846

2. Restriction fragment length polymorphism in canine narcolepsy.

Authors: M Motoyama; T S Kilduff; B S Lee; W C Dement; H O McDevitt
Journal: Immunogenetics Date: 1989 Impact factor: 2.846

3. DNA typing of HLA-DR beta chain genes can discriminate between undetected alleles and real homozygotes.

Authors: C de Préval; G Angelini; B Boogh; G B Ferrara; B Mach
Journal: Immunogenetics Date: 1987 Impact factor: 2.846

4. The genomic organisation and nucleotide sequence of the HLA-SB(DP) alpha gene.

Authors: S K Lawrance; H K Das; J Pan; S M Weissman
Journal: Nucleic Acids Res Date: 1985-10-25 Impact factor: 16.971

5. An isolated beta 1 exon next to the DR alpha gene in the HLA-D region.

Authors: H F Meunier; S Carson; W F Bodmer; J Trowsdale
Journal: Immunogenetics Date: 1986 Impact factor: 2.846

6. High-resolution analysis of the human HLA-DR polymorphism by hybridization with sequence-specific oligonucleotide probes.

Authors: G Angelini; C de Preval; J Gorski; B Mach
Journal: Proc Natl Acad Sci U S A Date: 1986-06 Impact factor: 11.205