Literature DB >> 3856233

Intracellular free calcium levels are reduced in mitotic Pt K2 epithelial cells.

C H Keith, F R Maxfield, M L Shelanski.   

Abstract

Using a fluorescence ratio method, we have studied the intracellular free calcium levels in individual quin-2-loaded mitotic cells under the microscope. We have found that intracellular free calcium concentrations in Pt K2 epithelial cells drop by approximately 50% as they pass through mitosis. Calcium levels in interphase cells were 53 +/- 7 nM. During prophase, free cytoplasmic calcium begins to decrease, reaching 28 +/- 3 nM in prometaphase. Calcium levels remain low until the nuclear envelope is re-formed in late telophase, when they increase again to interphase levels. This decrease in overall free calcium in mitosis suggests that the mitotic cell has mechanisms for the general sequestration, and perhaps local release, of calcium ions.

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Year:  1985        PMID： 3856233      PMCID： PMC397134          DOI： 10.1073/pnas.82.3.800

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  33 in total

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Journal:  Adv Cyclic Nucleotide Res       Date:  1979

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Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

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Journal:  Biochemistry       Date:  1975-07       Impact factor: 3.162

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Journal:  Exp Cell Res       Date:  1972-02       Impact factor: 3.905

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Authors:  E Nishida; H Sakai
Journal:  J Biochem       Date:  1977-07       Impact factor: 3.387

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Journal:  Proc Natl Acad Sci U S A       Date:  1976-02       Impact factor: 11.205

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Authors:  M Zavortink; M J Welsh; J R McIntosh
Journal:  Exp Cell Res       Date:  1983-12       Impact factor: 3.905

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Journal:  Cell       Date:  1980-02       Impact factor: 41.582

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Journal:  Biochemistry       Date:  1980-05-27       Impact factor: 3.162

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Authors:  M J Welsh; J R Dedman; B R Brinkley; A R Means
Journal:  J Cell Biol       Date:  1979-06       Impact factor: 10.539
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  14 in total

1.  Saccharomyces cerevisiae Dmc1 and Rad51 proteins preferentially function with Tid1 and Rad54 proteins, respectively, to promote DNA strand invasion during genetic recombination.

Authors:  Amitabh V Nimonkar; Christopher C Dombrowski; Joseph S Siino; Alicja Z Stasiak; Andrzej Stasiak; Stephen C Kowalczykowski
Journal:  J Biol Chem       Date:  2012-06-29       Impact factor: 5.157

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Authors:  C Petzelt; M Hafner
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 11.205

3.  Transition from metaphase to anaphase is accompanied by local changes in cytoplasmic free calcium in Pt K2 kidney epithelial cells.

Authors:  R R Ratan; M L Shelanski; F R Maxfield
Journal:  Proc Natl Acad Sci U S A       Date:  1986-07       Impact factor: 11.205

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Authors:  M Wahl; E Gruenstein
Journal:  Mol Biol Cell       Date:  1993-03       Impact factor: 4.138

5.  Cytosolic free calcium and cell spreading decrease in fibroblasts from aged and Alzheimer donors.

Authors:  C Peterson; R R Ratan; M L Shelanski; J E Goldman
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205

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Authors:  R R Ratan; F R Maxfield; M L Shelanski
Journal:  J Cell Biol       Date:  1988-09       Impact factor: 10.539

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Authors:  P K Hepler; D A Callaham
Journal:  J Cell Biol       Date:  1987-11       Impact factor: 10.539

8.  Reducing inositol lipid hydrolysis, Ins(1,4,5)P3 receptor availability, or Ca2+ gradients lengthens the duration of the cell cycle in Xenopus laevis blastomeres.

Authors:  J K Han; K Fukami; R Nuccitelli
Journal:  J Cell Biol       Date:  1992-01       Impact factor: 10.539

9.  Intracellular free calcium oscillates during cell division of Xenopus embryos.

Authors:  N Grandin; M Charbonneau
Journal:  J Cell Biol       Date:  1991-02       Impact factor: 10.539

10.  Organization of cytokeratin bundles by desmosomes in rat mammary cells.

Authors:  M Bologna; R Allen; R Dulbecco
Journal:  J Cell Biol       Date:  1986-02       Impact factor: 10.539
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