Literature DB >> 385310

Lack of correlation between affinity of the tRNA for the aminoacyl-tRNA synthetase and aminoacylation capacity as studied with modified tRNAPhe.

M Renaud, R Ehrlich, J Bonnet, P Remy.   

Abstract

The interactions of several modified yeast tRNAPhe [tRNAPhe lacking 7-methylguanine; a fragment comprising about 3/4 of the whole molecule: tRNAPhe (18--76); tRNAPhe (18--76) lacking 7-methylguanine] with yeast phenylalanyl-tRNA synthetase were studied. Upon excision of the 5'-quarter of the tRNAPhe molecule, the residual fragment still tightly binds to the synthetase, but can no longer by aminoacylated. Surprisingly, upon removal of the 7-methylguanine base at position 46 in this fragment, althought the affinity drops by a factor 10, a significant aminoacylation is restored. These results are discussed in terms of molecular flexibility and a model is proposed for tRNA-enzyme interaction, involving multisite recognition.

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Year:  1979        PMID： 385310     DOI： 10.1111/j.1432-1033.1979.tb02044.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  8 in total

1.  The structure of an AspRS-tRNA(Asp) complex reveals a tRNA-dependent control mechanism.

Authors:  L Moulinier; S Eiler; G Eriani; J Gangloff; J C Thierry; K Gabriel; W H McClain; D Moras
Journal:  EMBO J       Date:  2001-09-17       Impact factor: 11.598

Review 2.  The early history of tRNA recognition by aminoacyl-tRNA synthetases.

Authors:  Richard Giegé
Journal:  J Biosci       Date:  2006-10       Impact factor: 1.826

3.  tRNA-guanine transglycosylase from Escherichia coli: recognition of noncognate-cognate chimeric tRNA and discovery of a novel recognition site within the TpsiC arm of tRNA(Phe).

Authors:  F L Kung; S Nonekowski; G A Garcia
Journal:  RNA       Date:  2000-02       Impact factor: 4.942

4.  Conformational activation of the yeast phenylalanyl-tRNA synthetase catalytic site induced by tRNAPhe interaction: triggering of adenosine or CpCpA trinucleoside diphosphate aminoacylation upon binding of tRNAPhe lacking these residues.

Authors:  M Renaud; H Bacha; P Remy; J P Ebel
Journal:  Proc Natl Acad Sci U S A       Date:  1981-03       Impact factor: 11.205

5.  Chemical conversion of cytidine residues into 4-thiouridines in yeast tRNAPhe. Determination of the modified cytidines.

Authors:  N Riehl; P Carbon; B Ehresmann; J P Ebel
Journal:  Nucleic Acids Res       Date:  1984-06-11       Impact factor: 16.971

6.  Length requirements for tRNA-specific enzymes and cleavage specificity at the 3' end of turnip yellow mosaic virus RNA.

Authors:  S Joshi; F Chapeville; A L Haenni
Journal:  Nucleic Acids Res       Date:  1982-03-25       Impact factor: 16.971

7.  The tRNA-like structure of turnip yellow mosaic virus RNA: structural organization of the last 159 nucleotides from the 3' OH terminus.

Authors:  C Florentz; J P Briand; P Romby; L Hirth; J P Ebel; R Glegé
Journal:  EMBO J       Date:  1982       Impact factor: 11.598

Review 8.  tRNA biology in mitochondria.

Authors:  Thalia Salinas-Giegé; Richard Giegé; Philippe Giegé
Journal:  Int J Mol Sci       Date:  2015-02-27       Impact factor: 5.923
  8 in total

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