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Literature DB >> 6377234

Chemical conversion of cytidine residues into 4-thiouridines in yeast tRNAPhe. Determination of the modified cytidines.

N Riehl, P Carbon, B Ehresmann, J P Ebel.

Abstract

Treatment of yeast phenylalanine tRNA with pressurized hydrogen sulfide results in conversion of cytidine residues into 4-thiouridine residues. Under conditions leading to an average modification of one cytidine per tRNA molecule 9 positions are thiolated. The 4-thiouridine residues are distributed along the tRNA molecule. Four of the reactive cytidines are located in single-stranded regions: Cm32 , C60 , C74 and C75 . The five others are located in base pairs: C2, C27, C56 , C61 and C63 . Importance of replacement of an amino group by a thiol group on hydrogen bonding and on biological activity of the modified tRNA is discussed.

Entities: Chemical Species

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Year: 1984 PMID： 6377234 PMCID： PMC318849 DOI： 10.1093/nar/12.11.4445

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

20 in total

1. The use of nuclease P1 in sequence analysis of end group labeled RNA.

Authors: M Silberklang; A M Gillum; U L RajBhandary
Journal: Nucleic Acids Res Date: 1977-12 Impact factor: 16.971

2. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

3. Loss of methionine acceptor activity resulting from a base change in the anticodon of Escherichia coli formylmethionine transfer ribonucleic acid.

Authors: L H Schulman; J P Goddard
Journal: J Biol Chem Date: 1973-02-25 Impact factor: 5.157

4. Studies on tRNA nucleotidyltransferase from baker's yeast. 1. Purification of the enzyme. Protection against thermal inactivation and inhibition by several substrates.

Authors: B Rether; J Bonnet; J P Ebel
Journal: Eur J Biochem Date: 1974-12-16

Chemical conversion of cytidine residues into 4-thiouridines in yeast tRNAPhe. Determination of the modified cytidines.

1. The use of nuclease P1 in sequence analysis of end group labeled RNA.

2. A new method for sequencing DNA.

3. Loss of methionine acceptor activity resulting from a base change in the anticodon of Escherichia coli formylmethionine transfer ribonucleic acid.

4. Studies on tRNA nucleotidyltransferase from baker's yeast. 1. Purification of the enzyme. Protection against thermal inactivation and inhibition by several substrates.

5. UV shadowing--a new and convenient method for the location of ultraviolet-absorbing species in polyacrylamide gels.

6. Intramolecular cross-linking of single-stranded copolymers of 4-thiouridine and cytidine.

7. Sequential degradation of nucleic acids. Degradation of Escherichia coli B phenylalanine transfer ribonucleic acid.

8. Nucleosides and nucleotides. VI. Preparation of diribonucleoside monophosphates containing 4-thiouridine.

9. [Fractionation of Brewer's yeast t-RNA by countercurrent distribution].

10. Structural domains of transfer RNA molecules.

1. Use of specific chemical reagents for detection of modified nucleotides in RNA.