Literature DB >> 3840017

Construction and identification of a cDNA clone for human type II procollagen mRNA.

K Elima, J K Mäkelä, T Vuorio, S Kauppinen, J Knowles, E Vuorio.   

Abstract

Double-stranded cDNA was constructed for poly(A)-containing RNA isolated from foetal human articular cartilage known to contain small amounts of pro alpha 1 (II) collagen mRNA. A 585 base pair PstI-EcoRI cDNA fragment was isolated and cloned into plasmid pBR322. A resulting recombinant plasmid pHCAR1 was shown to hybridize specifically to a 5.4 kilobase mRNA in cartilage but not in calvarial RNA. Definite identification of clone pHCAR1 was based on sequence analysis; marked homology with the corresponding chick gene and complete agreement with the human gene sequences available were observed.

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Year:  1985        PMID： 3840017      PMCID： PMC1145165          DOI： 10.1042/bj2290183

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  27 in total

1.  An improved method for detecting foreign DNA in plasmids of Escherichia coli.

Authors:  R E Thayer
Journal:  Anal Biochem       Date:  1979-09-15       Impact factor: 3.365

2.  Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2.

Authors:  M Kahn; R Kolter; C Thomas; D Figurski; R Meyer; E Remaut; D R Helinski
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

3.  Correlation of procollagen mRNA levels in normal and transformed chick embryo fibroblasts with different rates of procollagen synthesis.

Authors:  D W Rowe; R C Moen; J M Davidson; P H Byers; P Bornstein; R D Palmiter
Journal:  Biochemistry       Date:  1978-05-02       Impact factor: 3.162

4.  The biosynthesis of collagen and its disorders (second of two parts).

Authors:  D J Prockop; K I Kivirikko; L Tuderman; N A Guzman
Journal:  N Engl J Med       Date:  1979-07-12       Impact factor: 91.245

5.  The biosynthesis of collagen and its disorders (first of two parts).

Authors:  D J Prockop; K I Kivirikko; L Tuderman; N A Guzman
Journal:  N Engl J Med       Date:  1979-07-05       Impact factor: 91.245

6.  Calcium-dependent bacteriophage DNA infection.

Authors:  M Mandel; A Higa
Journal:  J Mol Biol       Date:  1970-10-14       Impact factor: 5.469

7.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors:  P S Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

8.  Isolation of DNA from agarose gels using DEAE-paper. Application to restriction site mapping of adenovirus type 16 DNA.

Authors:  G Winberg; M L Hammarskjöld
Journal:  Nucleic Acids Res       Date:  1980-01-25       Impact factor: 16.971

9.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors:  H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  7 in total

1.  Structure of cDNA clones coding for human type II procollagen. The alpha 1(II) chain is more similar to the alpha 1(I) chain than two other alpha chains of fibrillar collagens.

Authors:  C T Baldwin; A M Reginato; C Smith; S A Jimenez; D J Prockop
Journal:  Biochem J       Date:  1989-09-01       Impact factor: 3.857

2.  Determination of the single polyadenylation site of the human pro alpha 1(II) collagen gene.

Authors:  K Elima; T Vuorio; E Vuorio
Journal:  Nucleic Acids Res       Date:  1987-11-25       Impact factor: 16.971

Review 3.  Collagen genes and inherited connective tissue disease.

Authors:  K S Cheah
Journal:  Biochem J       Date:  1985-07-15       Impact factor: 3.857

4.  SV40 large T antigen immortalization of human articular chondrocytes.

Authors:  B Benoit; S Thenet-Gauci; F Hoffschir; P Penfornis; S Demignot; M Adolphe
Journal:  In Vitro Cell Dev Biol Anim       Date:  1995-03       Impact factor: 2.416

5.  Coordinated regulation of type I and type III collagen production and mRNA levels of pro alpha 1(I) and pro alpha 2(I) collagen in cultured morphea fibroblasts.

Authors:  T Vuorio; J K Mäkelä; V M Kähäri; E Vuorio
Journal:  Arch Dermatol Res       Date:  1987       Impact factor: 3.017

6.  Co-expression of collagens II and XI and alternative splicing of exon 2 of collagen II in several developing human tissues.

Authors:  M M Sandberg; H E Hirvonen; K J Elima; E I Vuorio
Journal:  Biochem J       Date:  1993-09-01       Impact factor: 3.857

7.  Localization of types I, II, and III collagen mRNAs in developing human skeletal tissues by in situ hybridization.

Authors:  M Sandberg; E Vuorio
Journal:  J Cell Biol       Date:  1987-04       Impact factor: 10.539
  7 in total

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