Literature DB >> 3822249

Molecular cloning, expression and in situ hybridization of rat brain glutamic acid decarboxylase messenger RNA.

J F Julien, F Legay, S Dumas, M Tappaz, J Mallet.   

Abstract

A cDNA library was generated in the expression vector lambda GT11 from rat brain poly(A)+ RNAs and screened with a GAD antiserum. Two clones reacted positively. One of them was shown to express a GAD activity which was specifically trapped on anti-GAD immunogel and was inhibited by gamma-acetylenic-GABA. Blot hybridization analysis of RNAs from rat brain revealed a single 4 kilobases band. Preliminary in situ hybridizations showed numerous cells labelled by the GAD probe such as the Purkinje and stellate cells in the cerebellar cortex and the cells of the reticular thalamic nucleus.

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Year:  1987        PMID： 3822249     DOI： 10.1016/0304-3940(87)90013-9

Source DB:  PubMed          Journal:  Neurosci Lett        ISSN： 0304-3940            Impact factor:   3.046


  9 in total

Review 1.  The structural and functional heterogeneity of glutamic acid decarboxylase: a review.

Authors:  M G Erlander; A J Tobin
Journal:  Neurochem Res       Date:  1991-03       Impact factor: 3.996

2.  Structure and alternative promoters of the mouse glutamic acid decarboxylase 67 gene.

Authors:  Y Yanagawa; T Kobayashi; T Kamei; K Ishii; M Nishijima; A Takaku; S Tamura
Journal:  Biochem J       Date:  1997-09-01       Impact factor: 3.857

3.  Pattern of expression of glutamic acid decarboxylase mRNA in the developing rat brain.

Authors:  R W Bond; K R Jansen; D I Gottlieb
Journal:  Proc Natl Acad Sci U S A       Date:  1988-05       Impact factor: 11.205

4.  Assignment of the rat genes coding for dopa decarboxylase (DDC) and glutamic acid decarboxylases (GAD1 and GAD2).

Authors:  C Vassort; M Rivière; G Bruneau; F Gros; J Thibault; G Levan; J Szpirer; C Szpirer
Journal:  Mamm Genome       Date:  1993       Impact factor: 2.957

5.  Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene.

Authors:  D F Bu; M G Erlander; B C Hitz; N J Tillakaratne; D L Kaufman; C B Wagner-McPherson; G A Evans; A J Tobin
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-15       Impact factor: 11.205

6.  Molecular biology of IDDM.

Authors:  A Lernmark
Journal:  Diabetologia       Date:  1994-09       Impact factor: 10.122

7.  Presence of mRNA for glutamic acid decarboxylase in both excitatory and inhibitory neurons.

Authors:  Y Cao; K S Wilcox; C E Martin; T L Rachinsky; J Eberwine; M A Dichter
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-03       Impact factor: 11.205

8.  A very large number of GABAergic neurons are activated in the tuberal hypothalamus during paradoxical (REM) sleep hypersomnia.

Authors:  Emilie Sapin; Anne Bérod; Lucienne Léger; Paul A Herman; Pierre-Hervé Luppi; Christelle Peyron
Journal:  PLoS One       Date:  2010-07-26       Impact factor: 3.240

9.  Enhanced anxiety observed in cocaine withdrawn rats is associated with altered reactivity of the dorsomedial prefrontal cortex.

Authors:  Cynthia El Hage; Virginie Rappeneau; Adeline Etievant; Anne-Laure Morel; Hélène Scarna; Luc Zimmer; Anne Bérod
Journal:  PLoS One       Date:  2012-08-16       Impact factor: 3.240
  9 in total

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