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Literature DB >> 3800952

Aldehyde dehydrogenase catalyses acetaldehyde formation from 4-nitrophenyl acetate and NADH.

Abstract

Incubation of sheep liver cytoplasmic aldehyde dehydrogenase with the substrate 4-nitrophenyl [14C]acetate in the presence of NADH leads to the formation of 14C-labelled acetaldehyde. This observation strongly supports the idea that the esterase and dehydrogenase activities of the enzyme occur at the same site and involve the intermediacy of a common acyl-enzyme.

Entities: Chemical Species

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Year: 1986 PMID： 3800952 PMCID： PMC1147177 DOI： 10.1042/bj2380617

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

12 in total

1. Kinetic studies on the esterase activity of cytoplasmic sheep liver aldehyde dehydrogenase.

Authors: A K MacGibbon; S J Haylock; P D Buckley; L F Blackwell
Journal: Biochem J Date: 1978-06-01 Impact factor: 3.857

2. The kinetics of pig brain aldehyde dehydrogenase.

Authors: R J Duncan; K F Tipton
Journal: Eur J Biochem Date: 1971-10-26

3. Relationship between the mechanisms of the esterase and dehydrogenase activities of the cytoplasmic aldehyde dehydrogenase from sheep liver. An alternative view.

Authors: L F Blackwell; A F Bennett; P D Buckley
Journal: Biochemistry Date: 1983-08-02 Impact factor: 3.162

4. Aldehyde dehydrogenase. An enzyme with two distinct catalytic activities at a single type of active site.

Authors: R J Duncan
Journal: Biochem J Date: 1985-08-15 Impact factor: 3.857

5. Determination of acetaldehyde in blood using automated distillation and fluorometry.

Authors: A R Stowell; K E Crow; R M Greenway; R D Batt
Journal: Anal Biochem Date: 1978-02 Impact factor: 3.365

6. Studies on the interaction between disulfiram and sheep liver cytoplasmic aldehyde dehydrogenase.

Authors: T M Kitson
Journal: Biochem J Date: 1978-10-01 Impact factor: 3.857

7. Human liver aldehyde dehydrogenase. Esterase activity.

Authors: R S Sidhu; A H Blair
Journal: J Biol Chem Date: 1975-10-10 Impact factor: 5.157

8. Nicotinamide adenine dinucleotide activation of the esterase reaction of horse liver aldehyde dehydrogenase.

Authors: K Takahashi; H Weiner
Journal: Biochemistry Date: 1981-05-12 Impact factor: 3.162

9. The use of pH-gradient ion-exchange chromatography to separate sheep liver cytoplasmic aldehyde dehydrogenase from mitochondrial enzyme contamination, and observations on the interaction between the pure cytoplasmic enzyme and disulfiram.

Authors: F M Dickinson; G J Hart; T M Kitson
Journal: Biochem J Date: 1981-12-01 Impact factor: 3.857

10. The effects of Mg2+ on certain steps in the mechanisms of the dehydrogenase and esterase reactions catalysed by sheep liver aldehyde dehydrogenase. Support for the view that dehydrogenase and esterase activities occur at the same site on the enzyme.

Authors: F M Dickinson; G W Haywood
Journal: Biochem J Date: 1986-02-01 Impact factor: 3.857

11 in total

1. Effect of disulfiram on the pre-steady-state burst in the reactions of sheep liver cytoplasmic aldehyde dehydrogenase.

Authors: T M Kitson
Journal: Biochem J Date: 1987-12-15 Impact factor: 3.857

2. Evidence that the cytoplasmic aldehyde dehydrogenase-catalysed oxidation of aldehydes involves a different active-site group from that which catalyses the hydrolysis of 4-nitrophenyl acetate.

Authors: R L Motion; P D Buckley; A F Bennett; L F Blackwell
Journal: Biochem J Date: 1988-09-15 Impact factor: 3.857

3. Benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase II from Acinetobacter calcoaceticus. Substrate specificities and inhibition studies.

Authors: R W MacKintosh; C A Fewson
Journal: Biochem J Date: 1988-10-15 Impact factor: 3.857

Aldehyde dehydrogenase catalyses acetaldehyde formation from 4-nitrophenyl acetate and NADH.

1. Kinetic studies on the esterase activity of cytoplasmic sheep liver aldehyde dehydrogenase.

2. The kinetics of pig brain aldehyde dehydrogenase.

3. Relationship between the mechanisms of the esterase and dehydrogenase activities of the cytoplasmic aldehyde dehydrogenase from sheep liver. An alternative view.

4. Aldehyde dehydrogenase. An enzyme with two distinct catalytic activities at a single type of active site.

5. Determination of acetaldehyde in blood using automated distillation and fluorometry.

6. Studies on the interaction between disulfiram and sheep liver cytoplasmic aldehyde dehydrogenase.

7. Human liver aldehyde dehydrogenase. Esterase activity.

8. Nicotinamide adenine dinucleotide activation of the esterase reaction of horse liver aldehyde dehydrogenase.

9. The use of pH-gradient ion-exchange chromatography to separate sheep liver cytoplasmic aldehyde dehydrogenase from mitochondrial enzyme contamination, and observations on the interaction between the pure cytoplasmic enzyme and disulfiram.

10. The effects of Mg2+ on certain steps in the mechanisms of the dehydrogenase and esterase reactions catalysed by sheep liver aldehyde dehydrogenase. Support for the view that dehydrogenase and esterase activities occur at the same site on the enzyme.

1. Effect of disulfiram on the pre-steady-state burst in the reactions of sheep liver cytoplasmic aldehyde dehydrogenase.

2. Evidence that the cytoplasmic aldehyde dehydrogenase-catalysed oxidation of aldehydes involves a different active-site group from that which catalyses the hydrolysis of 4-nitrophenyl acetate.

3. Benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase II from Acinetobacter calcoaceticus. Substrate specificities and inhibition studies.

4. Studies of the esterase activity of cytosolic aldehyde dehydrogenase with resorufin acetate as substrate.

5. Identification of a catalytically essential nucleophilic residue in sheep liver cytoplasmic aldehyde dehydrogenase.

6. Aldehyde dehydrogenase. Covalent intermediate in aldehyde dehydrogenation and ester hydrolysis.

7. A comparison of nitrophenyl esters and lactones as substrates of cytosolic aldehyde dehydrogenase.

8. Probing the active site of cytoplasmic aldehyde dehydrogenase with a chromophoric reporter group.

9. Kinetics of p-nitrophenyl pivalate hydrolysis catalysed by cytoplasmic aldehyde dehydrogenase.

10. Reaction between sheep liver mitochondrial aldehyde dehydrogenase and various thiol-modifying reagents.