Literature DB >> 2018476

Identification of a catalytically essential nucleophilic residue in sheep liver cytoplasmic aldehyde dehydrogenase.

T M Kitson1, J P Hill, G G Midwinter.   

Abstract

Sheep liver cytoplasmic aldehyde dehydrogenase was labelled by reaction with the substrate p-nitrophenyl di[14C]methylcarbamate. After tryptic digestion and peptide fractionation the labelled residue was identified as Cys-302. This is the first unequivocal identification of the essential enzymic nucleophile in the esterase activity of aldehyde dehydrogenase. By implication, Cys-302 is probably also the residue that is acylated by aldehyde substrates and the first residue that is modified by disulfiram.

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Year:  1991        PMID： 2018476      PMCID： PMC1150034          DOI： 10.1042/bj2750207

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  25 in total

1.  Evidence for reactivity of serine-74 with trans-4-(N,N-dimethylamino)cinnamaldehyde during oxidation by the cytoplasmic aldehyde dehydrogenase from sheep liver.

Authors:  K M Loomes; G G Midwinter; L F Blackwell; P D Buckley
Journal:  Biochemistry       Date:  1990-02-27       Impact factor: 3.162

2.  Aldehyde dehydrogenase catalyses acetaldehyde formation from 4-nitrophenyl acetate and NADH.

Authors:  K M Loomes; T M Kitson
Journal:  Biochem J       Date:  1986-09-01       Impact factor: 3.857

3.  Relationship between the mechanisms of the esterase and dehydrogenase activities of the cytoplasmic aldehyde dehydrogenase from sheep liver. An alternative view.

Authors:  L F Blackwell; A F Bennett; P D Buckley
Journal:  Biochemistry       Date:  1983-08-02       Impact factor: 3.162

4.  The cytoplasmic isoenzyme of horse liver aldehyde dehydrogenase. Relationship to the corresponding human isoenzyme.

Authors:  H von Bahr-Lindström; J Hempel; H Jörnvall
Journal:  Eur J Biochem       Date:  1984-05-15

5.  Aldehyde dehydrogenase. An enzyme with two distinct catalytic activities at a single type of active site.

Authors:  R J Duncan
Journal:  Biochem J       Date:  1985-08-15       Impact factor: 3.857

6.  Mitochondrial aldehyde dehydrogenase from human liver. Primary structure, differences in relation to the cytosolic enzyme, and functional correlations.

Authors:  J Hempel; R Kaiser; H Jörnvall
Journal:  Eur J Biochem       Date:  1985-11-15

7.  Studies on the interaction between disulfiram and sheep liver cytoplasmic aldehyde dehydrogenase.

Authors:  T M Kitson
Journal:  Biochem J       Date:  1978-10-01       Impact factor: 3.857

8.  Aldehyde dehydrogenase from human liver. Primary structure of the cytoplasmic isoenzyme.

Authors:  J Hempel; H von Bahr-Lindström; H Jörnvall
Journal:  Eur J Biochem       Date:  1984-05-15

9.  Characterization of the coenzyme binding site of liver aldehyde dehydrogenase: differential reactivity of coenzyme analogues.

Authors:  H von Bahr-Lindström; R Jeck; C Woenckhaus; S Sohn; J Hempel; H Jörnvall
Journal:  Biochemistry       Date:  1985-10-08       Impact factor: 3.162

10.  Identification of a segment containing a reactive cysteine residue in human liver cytoplasmic aldehyde dehydrogenase (isoenzyme E1).

Authors:  J Hempel; R Pietruszko; P Fietzek; H Jörnvall
Journal:  Biochemistry       Date:  1982-12-21       Impact factor: 3.162
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  11 in total

1.  Modulation of the reactivity of the essential cysteine residue of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa.

Authors:  Lilian González-Segura; Roberto Velasco-García; Rosario A Muñoz-Clares
Journal:  Biochem J       Date:  2002-02-01       Impact factor: 3.857

2.  Substrate-specificity of benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase encoded by TOL plasmid pWW0. Metabolic and mechanistic implications.

Authors:  J P Shaw; F Schwager; S Harayama
Journal:  Biochem J       Date:  1992-05-01       Impact factor: 3.857

3.  Haloenol lactones as inactivators and substrates of aldehyde dehydrogenase.

Authors:  N Mukerjee; M Dryjanski; W Dai; J A Katzenellenbogen; R Pietruszko
Journal:  J Protein Chem       Date:  1996-10

4.  Studies of the esterase activity of cytosolic aldehyde dehydrogenase with resorufin acetate as substrate.

Authors:  T M Kitson; K E Kitson
Journal:  Biochem J       Date:  1997-03-15       Impact factor: 3.857

5.  Aldehyde dehydrogenase. Covalent intermediate in aldehyde dehydrogenation and ester hydrolysis.

Authors:  E E Blatter; D P Abriola; R Pietruszko
Journal:  Biochem J       Date:  1992-03-01       Impact factor: 3.857

6.  A comparison of nitrophenyl esters and lactones as substrates of cytosolic aldehyde dehydrogenase.

Authors:  T M Kitson; K E Kitson
Journal:  Biochem J       Date:  1996-05-15       Impact factor: 3.857

7.  Probing the active site of cytoplasmic aldehyde dehydrogenase with a chromophoric reporter group.

Authors:  T M Kitson; K E Kitson
Journal:  Biochem J       Date:  1994-05-15       Impact factor: 3.857

8.  Effect of some thiocarbamate compounds on aldehyde dehydrogenase and implications for the disulfiram ethanol reaction.

Authors:  T M Kitson
Journal:  Biochem J       Date:  1991-08-15       Impact factor: 3.857

9.  UDP-glucose dehydrogenase from bovine liver: primary structure and relationship to other dehydrogenases.

Authors:  J Hempel; J Perozich; H Romovacek; A Hinich; I Kuo; D S Feingold
Journal:  Protein Sci       Date:  1994-07       Impact factor: 6.725

10.  Crystallographic and spectroscopic snapshots reveal a dehydrogenase in action.

Authors:  Lu Huo; Ian Davis; Fange Liu; Babak Andi; Shingo Esaki; Hiroaki Iwaki; Yoshie Hasegawa; Allen M Orville; Aimin Liu
Journal:  Nat Commun       Date:  2015-01-07       Impact factor: 14.919
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