Literature DB >> 2253270

Functional analysis of the yeast genome: use of two-dimensional gel electrophoresis to detect genes in randomly cloned DNA sequences.

D Thoraval1, M Régnacq, P Neuville, H Boucherie.   

Abstract

Genes are overexpressed when present in yeast cells on multicopy plasmids. Taking advantage of the protein amplification which results from this overexpression, a method has been developed for large scale detection of yeast genes on randomly cloned DNA sequences. It is based on the analysis, by two-dimensional gel electrophoresis, of the proteins from yeast cells transformed with a yeast genomic DNA library constructed in a multicopy vector. We demonstrate here the applicability of this method for exploring the yeast genome. In addition, we report results which suggest that this method may also be useful for detecting regulatory genes.

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Year:  1990        PMID： 2253270     DOI： 10.1007/bf00318208

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  14 in total

1.  Host-plasmid interactions in Saccharomyces cerevisiae: effect of host ploidy on plasmid stability and copy number.

Authors:  A Spalding; M F Tuite
Journal:  J Gen Microbiol       Date:  1989-04

2.  Identification of Glycolytic Enzyme Polypeptides on the Two-Dimensional Protein Map of Saccharomyces cerevisiae and Application to the Study of Some Wine Yeasts.

Authors:  M Brousse; N Bataillé; H Boucherie
Journal:  Appl Environ Microbiol       Date:  1985-10       Impact factor: 4.792

3.  Two-dimensional gel analysis of yeast proteins: application to the study of changes in the levels of major polypeptides of Saccharomyces cerevisiae depending on the fermentable or nonfermentable nature of the carbon source.

Authors:  N Bataillé; D Thoraval; H Boucherie
Journal:  Electrophoresis       Date:  1988-11       Impact factor: 3.535

4.  Genetic map of Saccharomyces cerevisiae, edition 10.

Authors:  R K Mortimer; D Schild; C R Contopoulou; J A Kans
Journal:  Yeast       Date:  1989 Sep-Oct       Impact factor: 3.239

5.  Computerized, comprehensive databases of cellular and secreted proteins from normal human embryonic lung MRC-5 fibroblasts: identification of transformation and/or proliferation sensitive proteins.

Authors:  J E Celis; G P Ratz; P Madsen; B Gesser; J B Lauridsen; K P Hansen; S Kwee; H H Rasmussen; H V Nielsen; D Crüger
Journal:  Electrophoresis       Date:  1989-02       Impact factor: 3.535

6.  Two dimensional gel electrophoresis and computer analysis of proteins synthesized by clonal cell lines.

Authors:  J I Garrels
Journal:  J Biol Chem       Date:  1979-08-25       Impact factor: 5.157

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Construction of high copy yeast vectors using 2-microns circle sequences.

Authors:  J R Broach
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

9.  Transformation-sensitive and growth-related changes of protein synthesis in REF52 cells. A two-dimensional gel analysis of SV40-, adenovirus-, and Kirsten murine sarcoma virus-transformed rat cells using the REF52 protein database.

Authors:  J I Garrels; B R Franza
Journal:  J Biol Chem       Date:  1989-03-25       Impact factor: 5.157

10.  Synthesis of specific identified, phosphorylated, heat shock, and heat stroke proteins through the cell cycle of Saccharomyces cerevisiae.

Authors:  J R Ludwig; J J Foy; S G Elliott; C S McLaughlin
Journal:  Mol Cell Biol       Date:  1982-02       Impact factor: 4.272
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  1 in total

1.  Seed-protein variation in maritime pine (Pinus pinaster Ait.) revealed by two-dimensional electrophoresis: genetic determinism and construction of a linkage map.

Authors:  S Gerber; F Rodolphe; N Bahrman; P Baradat
Journal:  Theor Appl Genet       Date:  1993-01       Impact factor: 5.699
  1 in total

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