Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Propagation of restriction fragments from the mitochondrial DNA of Saccharomyces cerevisiae in E. coli by means of plasmid vectors.

Literature DB >> 379817

Propagation of restriction fragments from the mitochondrial DNA of Saccharomyces cerevisiae in E. coli by means of plasmid vectors.

P E Berg, A Lewin, T Christianson, M Rabinowitz.

Abstract

Some of the EcoRI fragments of yeast (Saccharomyces cerevisiae) mitochondrial DNA were cloned into E. coli using plasmid pMB9. The five smallest fragments in molecular weight appeared to be preferentially retained by E coli; partial fragments derived from larger mitochondrial DNA fragments were also found. One of the fragments, R7 (2.4 kb), may contain the OII gene. Cloned R7 DNA was stable under a variety of growth conditions, but showed some changes in molecular weight after transfer to different E. coli strains. Fragment R7 is transcribed in minicells, producing RNA that hybridizes specifically to mitochondrial DNA. Both DNA strands are transcribed, in contrast to the asymmetric transcription found in mitochondria. No new polypeptides were observed in minicells containing cloned fragment 7.

Entities: Species

Mesh：

Substances：

Year: 1979 PMID： 379817 PMCID： PMC327841 DOI： 10.1093/nar/6.6.2133

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

58 in total

9. Heat shock protein HSP60 can alleviate the phenotype of mitochondrial RNA-deficient temperature-sensitive mna2 pet mutants.

Authors: A Sanyal; A Harington; C J Herbert; O Groudinsky; P P Slonimski; B Tung; G S Getz
Journal: Mol Gen Genet Date: 1995-01-06

9 in total

Propagation of restriction fragments from the mitochondrial DNA of Saccharomyces cerevisiae in E. coli by means of plasmid vectors.

1. Deg phenotype of Escherichia coli lon mutants.

2. End labeling of enzymatically decapped mRNA.

3. The sequence arrangement of Drosophila melanogaster 5s DNA cloned in recombinant plasmids.

4. Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.

5. Synthesis of hybrid bacterial plasmids containing highly repeated satellite DNA.

6. Protein expression in E. coli minicells by recombinant plasmids.

7. Transcription and translation of cloned Drosophila DNA fragments in Escherichia coli.

8. Expression in Escherichia coli K-12 of the structural gene for catabolic dehydroquinase of Neurospora crassa.

9. Synthesis of an ovalbumin-like protein by Escherichia coli K12 harbouring a recombinant plasmid.

10. Translation of Drosophila melanogaster sequences in Escherichia coli.

1. Measure of asymmetrical transcription of the yeast OMP decarboxylase gene expressed in yeast or in E. coli.

2. The cloning of Aspergillus nidulans mitochondrial DNA in Escherichia coli on plasmid pBR322.

3. Autocatalytic activities of intron 5 of the cob gene of yeast mitochondria.

4. Identification of a single transcriptional initiation site for the glutamic tRNA and COB genes in yeast mitochondria.

5. Variable abundance of a mitochondrial DNA fragment in cultured tobacco cells.

6. Temperature sensitive pet mutants in yeast Saccharomyces cerevisiae that lose mitochondrial RNA.

7. Expression of cloned mitochondrial DNA from the petite negative yeast Schizosaccharomyces pombe in E. coli minicells.

8. Processing of precursors of 21S ribosomal RNA from yeast mitochondria.

9. Heat shock protein HSP60 can alleviate the phenotype of mitochondrial RNA-deficient temperature-sensitive mna2 pet mutants.