Measure of asymmetrical transcription of the yeast OMP decarboxylase gene expressed in yeast or in E. coli.

The yeast OMP decarboxylase gene has been cloned in the phage fd in both orientations to obtain pure DNA of each strand. With these probes the transcription of each strand of the yeast gene was measured in wild type yeast, either repressed or induced, in a yeast strain transformed by an hybrid plasmid containing the OMPdecase gene, and in E. coli strains bearing the yeast gene cloned in different plasmids. In yeast it appears that in all conditions tested transcription was asymmetrical, 95% being transcribed from one strand. In E. coli, transcription of the yeast gene occurred on both strands.