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Literature DB >> 3786135

Sequencing and identification of a cDNA clone for tomato polygalacturonase.

D Grierson, G A Tucker, J Keen, J Ray, C R Bird, W Schuch.

Abstract

The 2a isoenzyme of tomato polygalacturonase was purified from ripe fruit and characterised. The N-terminal amino acid sequence of the protein was determined in order to identify polygalacturonase cDNA clones. The nucleotide sequence of a ripening-related cDNA (pTOM 6) was determined and found to encode the N-terminal sequence of mature polygalacturonase 2a. The complete open reading frame encodes a polypeptide of molecular weight 50,051, including a putative pre-sequence of 71 amino acids.

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Year: 1986 PMID： 3786135 PMCID： PMC311879 DOI： 10.1093/nar/14.21.8595

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

13 in total

1. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

Authors: R A REISFELD; U J LEWIS; D E WILLIAMS
Journal: Nature Date: 1962-07-21 Impact factor: 49.962

2. mRNA transcripts of several plant genes are polyadenylated at multiple sites in vivo.

Authors: C Dean; S Tamaki; P Dunsmuir; M Favreau; C Katayama; H Dooner; J Bedbrook
Journal: Nucleic Acids Res Date: 1986-03-11 Impact factor: 16.971

3. Patterns of amino acids near signal-sequence cleavage sites.

Authors: G von Heijne
Journal: Eur J Biochem Date: 1983-06-01

4. A simple method for displaying the hydropathic character of a protein.

Authors: J Kyte; R F Doolittle
Journal: J Mol Biol Date: 1982-05-05 Impact factor: 5.469

5. The conversion of tomato-fruit polygalacturonase isoenzyme 2 into isoenzyme 1 in vitro.

Authors: G A Tucker; N G Robertson; D Grierson
Journal: Eur J Biochem Date: 1981-03-16

6. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.

Authors: R Staden
Journal: Nucleic Acids Res Date: 1982-08-11 Impact factor: 16.971

7. Degradation of isolated tomato cell walls by purified polygalacturonase in vitro.

Authors: A P Themmen; G A Tucker; D Grierson
Journal: Plant Physiol Date: 1982-01 Impact factor: 8.340

8. Changes in polygalacturonase isoenzymes during the 'ripening' of normal and mutant tomato fruit.

Authors: G A Tucker; N G Robertson; D Grierson
Journal: Eur J Biochem Date: 1980-11

9. DNA sequencing with chain-terminating inhibitors.

Authors: F Sanger; S Nicklen; A R Coulson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

10. Modification of ovine opsin with the photosensitive hydrophobic probe 1-azido-4-[125I]iodobenzene. Labelling of the chromophore-attachment domain.

Authors: M D Davison; J B Findlay
Journal: Biochem J Date: 1986-03-01 Impact factor: 3.857

57 in total

10. Polygalacturonase gene expression in ripe melon fruit supports a role for polygalacturonase in ripening-associated pectin disassembly.

Authors: K A Hadfield; J K Rose; D S Yaver; R M Berka; A B Bennett
Journal: Plant Physiol Date: 1998-06 Impact factor: 8.340

Sequencing and identification of a cDNA clone for tomato polygalacturonase.

1. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

2. mRNA transcripts of several plant genes are polyadenylated at multiple sites in vivo.

3. Patterns of amino acids near signal-sequence cleavage sites.

4. A simple method for displaying the hydropathic character of a protein.

5. The conversion of tomato-fruit polygalacturonase isoenzyme 2 into isoenzyme 1 in vitro.

6. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.

7. Degradation of isolated tomato cell walls by purified polygalacturonase in vitro.

8. Changes in polygalacturonase isoenzymes during the 'ripening' of normal and mutant tomato fruit.

9. DNA sequencing with chain-terminating inhibitors.

10. Modification of ovine opsin with the photosensitive hydrophobic probe 1-azido-4-[125I]iodobenzene. Labelling of the chromophore-attachment domain.

1. Polygalacturonase gene expression in kiwifruit: relationship to fruit softening and ethylene production.

2. Sequence analysis of three members of the maize polygalacturonase gene family expressed during pollen development.

3. Suppression of Virus Accumulation in Transgenic Plants Exhibiting Silencing of Nuclear Genes.

4. Codon usage in plant genes.

Review 5. Molecular biology of fruit ripening and its manipulation with antisense genes.

6. Tomato Fruit Polygalacturonase Isozyme 1 (Characterization of the [beta] Subunit and Its State of Assembly in Vivo).

7. Isolation and characterization of a polygalacturonase gene highly expressed in Brassica napus pollen.

8. A wound- and systemin-inducible polygalacturonase in tomato leaves.

9. Cloning of a tomato polygalacturonase expressed in abscission.

10. Polygalacturonase gene expression in ripe melon fruit supports a role for polygalacturonase in ripening-associated pectin disassembly.