Standardized one- and two-dimensional thin-layer chromatographic methods for the identification of secondary metabolites in Penicillium and other fungi.

Standardized thin-layer chromatographic (TLC) data in two solvent systems are presented for secondary metabolites of Penicillium and other fungi to assist in the identification of products of Penicillium species, based on a study of 304 strains. Rapid identification aids are needed to check for activity during preservation for mycotoxin production, and for screening systematic and biotechnological purposes. The data are stored on a microcomputer which permits flexible storage, retrieval, and updating of information. Of 107 metabolites detected with TLC system 1 [solvent: toluene-ethyl acetate-90% formic acid (5:4:1, v/v/v)], 80 (75%) are named and 27 (25%) as yet unidentified compounds are alloted reference numbers; in the case of the 97 metabolites detected by system 2 [solvent: chloroform-acetone-propan-2-ol (85:15:20, v/v/v)] the equivalent figures are 79 (81%) and 18 (19%) respectively.