Literature DB >> 2730153

Analysis and screening for mycotoxins and other secondary metabolites in fungal cultures by thin-layer chromatography and high-performance liquid chromatography.

J C Frisvad, O Filtenborg, U Thrane.   

Abstract

Methods for the screening of fungal cultures for toxic secondary metabolites are reviewed. Thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) are good general analytical methods for secondary metabolites in unpurified extracts. The combination of normal phase TLC using different chemical spray reagents with reversed phase HPLC, using alkylphenone retention indices and diode array detection, is a powerful technique for identifying the individual mycotoxins detected. The results of the screening methods are very dependent of the growth media and incubation conditions and a general method for the detection of Penicillium, Aspergillus, Fusarium, Alternaria and Cladosporium toxins is suggested.

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Year:  1989        PMID: 2730153     DOI: 10.1007/bf01062357

Source DB:  PubMed          Journal:  Arch Environ Contam Toxicol        ISSN: 0090-4341            Impact factor:   2.804


  11 in total

1.  Systematic analysis of mycotoxins by thin-layer chromatography.

Authors:  Z Duracková; V Betina; P Nemec
Journal:  J Chromatogr       Date:  1976-01-07

2.  Standardized one- and two-dimensional thin-layer chromatographic methods for the identification of secondary metabolites in Penicillium and other fungi.

Authors:  R R Paterson
Journal:  J Chromatogr       Date:  1986-10-24

3.  Standardized high-performance liquid chromatography of 182 mycotoxins and other fungal metabolites based on alkylphenone retention indices and UV-VIS spectra (diode array detection).

Authors:  J C Frisvad; U Thrane
Journal:  J Chromatogr       Date:  1987-08-28

4.  The separation and detection of several mycotoxins by thin-layer chromatography.

Authors:  P S Steyn
Journal:  J Chromatogr       Date:  1969-12-23

5.  High-performance liquid chromatographic determination of profiles of mycotoxins and other secondary metabolites.

Authors:  J C Frisvad
Journal:  J Chromatogr       Date:  1987-04-17

6.  Screening methods for the detection of thirteen common mycotoxins.

Authors:  C P Gorst-Allman; P S Steyn
Journal:  J Chromatogr       Date:  1979-07-13

7.  Simple screening method for molds producing intracellular mycotoxins in pure cultures.

Authors:  O Filtenborg; J C Frisvad; J A Svendsen
Journal:  Appl Environ Microbiol       Date:  1983-02       Impact factor: 4.792

8.  A convenient thin layer chromatographic method for the detection of cyclochlorotine and simatoxin, toxins from Penicillium islandicum Sopp.

Authors:  A C Ghosh; A Manmade; A Bousquet; J M Townsend; A L Demain
Journal:  Experientia       Date:  1978-06-15

9.  Brine shrimp (Artemia salina L.) larvae as a screening system for fungal toxins.

Authors:  J Harwig; P M Scott
Journal:  Appl Microbiol       Date:  1971-06

10.  Classification of terverticillate penicillia based on profiles of mycotoxins and other secondary metabolites.

Authors:  J C Frisvad; O Filtenborg
Journal:  Appl Environ Microbiol       Date:  1983-12       Impact factor: 4.792

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  8 in total

1.  Rapid method to estimate the presence of secondary metabolites in microbial extracts.

Authors:  R E Higgs; J A Zahn; J D Gygi; M D Hilton
Journal:  Appl Environ Microbiol       Date:  2001-01       Impact factor: 4.792

2.  Nephrotoxigenic Penicillium species occurring on farm-stored cereal grains in western Canada.

Authors:  J T Mills; K A Seifert; J C Frisvad; D Abramson
Journal:  Mycopathologia       Date:  1995       Impact factor: 2.574

3.  Field ecology of the ochratoxin A-producing Penicillium verrucosum: survival and resource colonisation in soil.

Authors:  S Elmholt; H Hestbjerg
Journal:  Mycopathologia       Date:  1999       Impact factor: 2.574

4.  Classification of Aspergillus, Penicillium, Talaromyces and related genera (Eurotiales): An overview of families, genera, subgenera, sections, series and species.

Authors:  J Houbraken; S Kocsubé; C M Visagie; N Yilmaz; X-C Wang; M Meijer; B Kraak; V Hubka; K Bensch; R A Samson; J C Frisvad
Journal:  Stud Mycol       Date:  2020-06-27       Impact factor: 16.097

5.  Occurrence of toxigenic Aspergillus versicolor isolates and sterigmatocystin in carpet dust from damp indoor environments.

Authors:  Steffen Engelhart; Annette Loock; Dirk Skutlarek; Helmut Sagunski; Annette Lommel; Harald Färber; Martin Exner
Journal:  Appl Environ Microbiol       Date:  2002-08       Impact factor: 4.792

6.  Fungal Species and Multi-Mycotoxin Associated with Post-Harvest Sorghum (Sorghum bicolor (L.) Moench) Grain in Eastern Ethiopia.

Authors:  Abdi Mohammed; Zelalem Bekeko; Mawardi Yusufe; Michael Sulyok; Rudolf Krska
Journal:  Toxins (Basel)       Date:  2022-07-11       Impact factor: 5.075

7.  Aspergillus niger Environmental Isolates and Their Specific Diversity Through Metabolite Profiling.

Authors:  Alexandra Šimonovičová; Hana Vojtková; Sanja Nosalj; Elena Piecková; Hana Švehláková; Lucia Kraková; Hana Drahovská; Barbara Stalmachová; Kateřina Kučová; Domenico Pangallo
Journal:  Front Microbiol       Date:  2021-06-23       Impact factor: 5.640

8.  Characterization of Blue Mold Penicillium Species Isolated from Stored Fruits Using Multiple Highly Conserved Loci.

Authors:  Guohua Yin; Yuliang Zhang; Kayla K Pennerman; Guangxi Wu; Sui Sheng T Hua; Jiujiang Yu; Wayne M Jurick; Anping Guo; Joan W Bennett
Journal:  J Fungi (Basel)       Date:  2017-03-01
  8 in total

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