Literature DB >> 377231

Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin-Dahlem, GFR.

C Zwieb, R Brimacombe.   

Abstract

It is well established that when E. coli 30S ribosomal subunits are irradiated with ultraviolet light under mild conditions a specific cross-link is formed between protein S7 and the 16S RNA. Methodology is presented for the analysis of the single nucleotide residue concerned in this cross-link. Firstly, the identity of the ribonuclease T1 octanucleotide attached to S7 is confirmed by a new method, which involves isolation and analysis of S7-polynucleotide complexes containing 30 -- 40 nucleotides. Secondly, the isolated S7-octanucleotide complex is digested successively with ribonuclease A, proteinase K and ribonuclease T2, and the nucleotides liberated are identified. The results show unambiguously that uridine residue number 1239 in the 16S RNA sequence is cross-linked to protein S7.

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Year:  1979        PMID： 377231      PMCID： PMC327810          DOI： 10.1093/nar/6.5.1775

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  24 in total

1.  Hydrolysis of polyadenylic acid by pancreatic ribonuclease.

Authors:  R F BEERS
Journal:  J Biol Chem       Date:  1960-08       Impact factor: 5.157

2.  30S ribosomal proteins cross-linked to 16S RNA by periodate oxidation followed by borohydride reduction.

Authors:  J Rinke; R Brimacombe
Journal:  Mol Biol Rep       Date:  1978-10-16       Impact factor: 2.316

3.  RNA-protein neighbourhoods of the ribosome obtained by crosslinking.

Authors:  H G Bäumert; S E Sköld; C G Kurland
Journal:  Eur J Biochem       Date:  1978-09-01

4.  Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.

Authors:  J Brosius; M L Palmer; P J Kennedy; H F Noller
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

5.  The primary structure of ribosomal protein S7 from E. coli strains K and B.

Authors:  J Reinbolt; D Tritsch
Journal:  FEBS Lett       Date:  1978-07-15       Impact factor: 4.124

6.  The sequence of Escherichia coli ribosomal 16 S RNA determined by new rapid gel methods.

Authors:  P Carbon; C Ehresmann; B Ehresmann; J P Ebel
Journal:  FEBS Lett       Date:  1978-10-01       Impact factor: 4.124

7.  Identification of the oligonucleotide and oligopeptide involved in an RNA--protein crosslink induced by ultraviolet irradiation of Escherichia coli 30 S ribosomal subunits.

Authors:  K Möller; C Zwieb; R Brimacombe
Journal:  J Mol Biol       Date:  1978-12-15       Impact factor: 5.469

8.  Recent progress in the determination of the primary sequence of the 16 S RNA of Escherichia coli.

Authors:  C Ehresmann; P Stiegler; P Carbon; J P Ebel
Journal:  FEBS Lett       Date:  1977-12-15       Impact factor: 4.124

9.  The use of formaldehyde in RNA-protein cross-linking studies with ribosomal subunits from Escherichia coli.

Authors:  K Möller; J Rinke; A Ross; G Buddle; R Brimacombe
Journal:  Eur J Biochem       Date:  1977-06-01

10.  The use of sym-triazine trichloride in RNA-protein cross-linking studies with Escherichia coli ribosomal subunits.

Authors:  C Oste; R Brimacombe
Journal:  Mol Gen Genet       Date:  1979-01-05
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  21 in total

1.  Mining biochemical information: lessons taught by the ribosome.

Authors:  Michelle Whirl-Carrillo; Irene S Gabashvili; Michael Bada; D Rey Banatao; Russ B Altman
Journal:  RNA       Date:  2002-03       Impact factor: 4.942

2.  Selective isolation and detailed analysis of intra-RNA cross-links induced in the large ribosomal subunit of E. coli: a model for the tertiary structure of the tRNA binding domain in 23S RNA.

Authors:  P Mitchell; M Osswald; D Schueler; R Brimacombe
Journal:  Nucleic Acids Res       Date:  1990-08-11       Impact factor: 16.971

3.  Multiple crosslinks of proteins S7, S9, S13 to domains 3 and 4 of 16S RNA in the 30S particle.

Authors:  E Hajnsdorf; A Favre; A Expert-Bezançon
Journal:  Nucleic Acids Res       Date:  1986-05-27       Impact factor: 16.971

4.  hnRNP A1 binds promiscuously to oligoribonucleotides: utilization of random and homo-oligonucleotides to discriminate sequence from base-specific binding.

Authors:  N Abdul-Manan; K R Williams
Journal:  Nucleic Acids Res       Date:  1996-10-15       Impact factor: 16.971

5.  The environment of 5S rRNA in the ribosome: cross-links to the GTPase-associated area of 23S rRNA.

Authors:  P Sergiev; S Dokudovskaya; E Romanova; A Topin; A Bogdanov; R Brimacombe; O Dontsova
Journal:  Nucleic Acids Res       Date:  1998-06-01       Impact factor: 16.971

Review 6.  Structure and function of ribosomal RNA.

Authors:  R Brimacombe; W Stiege
Journal:  Biochem J       Date:  1985-07-01       Impact factor: 3.857

Review 7.  Contact-site cross-linking agents.

Authors:  G R Kunkel; M Mehrabian; H G Martinson
Journal:  Mol Cell Biochem       Date:  1981-01-20       Impact factor: 3.396

8.  Proteins crosslinked to messenger RNA by irradiating polyribosomes with ultraviolet light.

Authors:  J R Greenberg
Journal:  Nucleic Acids Res       Date:  1980-12-11       Impact factor: 16.971

9.  Localisation of a series of intra-RNA cross-links in 16S RNA, induced by ultraviolet irradiation of Escherichia coli 30S ribosomal subunits.

Authors:  C Zwieb; R Brimacombe
Journal:  Nucleic Acids Res       Date:  1980-06-11       Impact factor: 16.971

10.  An experimentally-derived model for the secondary structure of the 16S ribosomal RNA from Escherichia coli.

Authors:  C Glotz; R Brimacombe
Journal:  Nucleic Acids Res       Date:  1980-06-11       Impact factor: 16.971
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